Toxic mechanism on phenanthrene-induced cytotoxicity, oxidative stress and activity changes of superoxide dismutase and catalase in earthworm (Eisenia foetida): A combined molecular and cellular study

He, Falin; Liu, Qiang; Jing, Mingyang; Wan, Jingqiang; Huo, Chengqian; Zong, Wansong; Tang, Jingchun; Liu, Rutao

doi:10.1016/j.jhazmat.2021.126302

Cited by 87 publications

(9 citation statements)

References 84 publications

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“…This indicates that LIPSS processing leads to the formation of TiO 2 , Al 2 O 3 , and VO 2 on the TC4 surface. These XPS spectra show that the laser irradiation process oxidizes surface elements when inducing LIPSS. , However, surface oxides will also change the surface biomedical performance. , …”

Section: Resultsmentioning

confidence: 99%

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Antibacterial Performance of Zr-BMG, Stainless Steel, and Titanium Alloy with Laser-Induced Periodic Surface Structures

Wang

Zhang

et al. 2021

ACS Appl. Bio Mater.

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A laser-induced periodic surface structure (LIPSS) was shown to have antibacterial adhesion properties in previous research. In this study, the antibacterial performance of LIPSS on traditional biometals (stainless steel and titanium alloy) and a potential biometal (zirconium-based bulk metallic glass, Zr-BMG) was investigated. A femtosecond laser was used to fabricate LIPSS on the specimens. Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) were used to examine the antibacterial behavior of the LIPSS samples. The bacterial adhesion force on each specimen was evaluated by an atomic force microscopy (AFM) cell probe. The results showed that the LIPSS on all three metal surfaces significantly lowered antibacterial adhesion compared to polished metal specimens. E. coli demonstrated a higher adhesion force but a lower surface adhesion rate compared to S. aureus. The Zr-BMG specimen with LIPSS has multiple antimicrobial mechanisms (physical antiadhesion and chemical elimination), while the traditional biometals (316L and TC4) mainly offer physical antiadhesion. Finally, an in vitro/vivo study showed that specimens with LIPSS surfaces did not significantly affect the biocompatibility of the specimens. This study reveals that the Zr-BMG specimen with femtosecond laser-processed LIPSS is an ideal choice for achieving an antibacterial surface.

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Section: Resultsmentioning

confidence: 99%

“…23,24 However, surface oxides will also change the surface biomedical performance. 25,26 3.3. Surface Roughness.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Performance of Zr-BMG, Stainless Steel, and Titanium Alloy with Laser-Induced Periodic Surface Structures

Wang

Zhang

et al. 2021

ACS Appl. Bio Mater.

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“…ROS plays a major role in normal cell signaling, and dysregulation of ROS can alter the expression of genes activated by redox mechanisms [ 27 ]. Excess ROS in cells may cause mitochondrial dysfunction [ 28 ], induce lipid peroxidation [ 29 ] and DNA strand breaks [ 30 ], and ultimately lead to cell death. Therefore, the activity changes of antioxidant enzymes were measured next.…”

Section: Resultsmentioning

confidence: 99%

“…The content of MDA is often used as an indicator of lipid peroxidation. Therefore, the degree of oxidative damage to cells can be understood through the content of MDA [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Cytotoxicity and Oxidative Stress Effects of Indene on Coelomocytes of Earthworm (Eisenia foetida): Combined Analysis at Cellular and Molecular Levels

Huo

Qiang²,

Liu

et al. 2023

Toxics

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Indene (IND) is a kind of important aromatic hydrocarbon that is extracted from coal tar and has important applications in industry and biology. In the process of production and utilization, it is easy to enter the soil and produce toxic effects on the soil or organisms. The earthworm is an important organism in the soil. The toxicity of indene on earthworm coelomocytes is rarely studied, and the oxidative stress effects of IND on earthworm coelomocytes remain unclear. In this study, coelomocytes from earthworms and antioxidant enzymes were selected as the research targets. In addition, IND caused oxidative stress, and its related toxic effects and mechanisms were systematically studied and evaluated at the cellular and molecular levels. The results showed that IND destroyed the redox balance in earthworm coelomocytes, and the large accumulation of reactive oxygen species (ROS) significantly inhibited the activities of the antioxidant system, including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), and caused lipid peroxidation and membrane permeability changes, resulting in a decrease in cell viability to 74.5% of the control group. At the molecular level, IND was bound to SOD by the arene-H bond, and the binding constant was 4.95 × 103. IND changed the secondary structure of the SOD and led to a loosening of the structure of the SOD peptide chain. Meanwhile, IND caused SOD fluorescence sensitization, and molecular simulation showed that IND was mainly bound to the junction of SOD subunits. We hypothesized that the changes in SOD structure led to the increase in SOD activity. This research can provide a scientific basis for IND toxicity evaluation.

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“…PM and PM-bound chemicals such as PAHs and heavy metals have been reported to cause oxidative stress in plants [15,16]. Formation of reactive oxygen species (ROS) can trigger membrane and cell damages [17].…”

Section: Introductionmentioning

confidence: 99%

Effects of urban atmospheric particulate matter on higher plants using Lycopersicon esculentum as model species

2021

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Atmospheric particulate matter (PM) is one of the major environmental concerns in Europe. A wide range of studies has proved the ecotoxic potential of atmospheric particles. PM exerts chemical stress on vegetation by its potentially toxic constituents; however, relatively few studies are available on assessing phytotoxic effects under laboratory conditions. In our study, aqueous extract of particulate matter was prepared and used for treatment. Experiment was following the procedure defined by the No. 227 OECD Guideline for the Testing of Chemicals: Terrestrial Plant Test. Tomato (Lycopersicon esculentum Mill.) plants were used; elucidated toxicity was assessed based on morphological and biochemical endpoints such as biomass, chlorophyll-a and chlorophyll-b, carotenoids, and protein content. Biomass reduction and protein content showed a clear dose–effect relationship; the biomass decreased in comparison with the control (100%) in all test groups (TG) at a steady rate (TG1: 87.73%; TG2: 71.77%; TG3: 67.01%; TG4: 63.63%). The tendency in protein concentrations compared to the control was TG1: 113.61%; TG2: 148.21% TG3: 160.52%; TG4: 157.31%. However, pigments showed a ‘Janus-faced’ effect: nutrient content of the sample caused slight increase at lower doses; actual toxicity became apparent only at higher doses (chlorophyll-a concentration decrease was 84.47% in TG4, chlorophyll-b was 77.17%, and finally, carotene showed 83.60% decrease in TG4).

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Toxic mechanism on phenanthrene-induced cytotoxicity, oxidative stress and activity changes of superoxide dismutase and catalase in earthworm (Eisenia foetida): A combined molecular and cellular study

Cited by 87 publications

References 84 publications

Antibacterial Performance of Zr-BMG, Stainless Steel, and Titanium Alloy with Laser-Induced Periodic Surface Structures

Antibacterial Performance of Zr-BMG, Stainless Steel, and Titanium Alloy with Laser-Induced Periodic Surface Structures

Cytotoxicity and Oxidative Stress Effects of Indene on Coelomocytes of Earthworm (Eisenia foetida): Combined Analysis at Cellular and Molecular Levels

Effects of urban atmospheric particulate matter on higher plants using Lycopersicon esculentum as model species

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