Toxic Threats to Neurologic Development of Children

Schettler, Ted

doi:10.2307/3454643

Cited by 62 publications

(62 citation statements)

References 24 publications

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“…The measurement of all possible metabolites of PCBs is not realistic. Second, although there are limited available data describing the neurotoxicity of pesticides in humans, these chemicals may indeed affect the neurodevelopment of children (Schettler 2001). In the present study, however, the assay methods for pesticides including organochlorine and organophosphorus chemicals are not yet determined because the number of chemicals is too large.…”

Section: Resultsmentioning

confidence: 70%

The Tohoku Study of Child Development: A Cohort Study of Effects of Perinatal Exposures to Methylmercury and Environmentally Persistent Organic Pollutants on Neurobehavioral Development in Japanese Children

Nakai

Suzuki

Oka

et al. 2004

Tohoku J. Exp. Med.

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Section: Resultsmentioning

confidence: 70%

The Tohoku Study of Child Development: A Cohort Study of Effects of Perinatal Exposures to Methylmercury and Environmentally Persistent Organic Pollutants on Neurobehavioral Development in Japanese Children

Nakai

Suzuki

Oka

et al. 2004

Tohoku J. Exp. Med.

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“…36). In this context, it is important to note that attention-deficit hyperactivity disorder has been linked to disorders involving lipid metabolism 38 , pesticide exposure 28,29 and changes in ionchannel conductance in rat models 39 . Taken together, this evidence suggests that in mammals, the catalytic activity of NTE is linked to the control of motor activity and that the inhibition of NTE in humans may contribute to some hyperactivity disorders.…”

Section: Figmentioning

confidence: 99%

Loss of neuropathy target esterase in mice links organophosphate exposure to hyperactivity

et al. 2003

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“…In addition, the nonsteroidal chemicals procymidone and PCB blocked the translocation of AR into the nucleus. PCB and procymidone have been suspected of neurotoxic and antiandrogenic effects, respectively, and possibly influence adversely hormonal activities in the brains of living animals (15)(16)(17)(18). In the present study, we showed the usefulness of the split Rluc reporter for monitoring AR translocation into the nucleus in living mice by measuring bioluminescence with a cooled CCD camera.…”

Section: Discussionmentioning

confidence: 82%

High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of splitRenillaluciferase

Kim

Ozawa

Watanabe

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

123

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Nucleocytoplasmic trafficking of proteins in response to extraor intracellular stimuli is an essential step for regulating the magnitude and specificity of gene expressions. The trafficking is regulated by posttranslational modifications of proteins, which include ligand-receptor binding, protein phosphorylation, and proteolysis. The nuclear localization of those proteins is altered in the cells that are exposed to specific exogenous chemicals, of which potential effects on living animals are the major concern. Also, because various nuclear proteins are mislocalized in cancer cells, there is an intense interest in identifying small molecules that redirect the proteins to the correct compartments. Hence, development of a rapid screening system to detect the nucleocytoplasmic trafficking is essential for the discovery of novel compounds that have anticancer activity or for testing the toxicity of chemicals, from which new insights into the mechanism of nucleocytoplasmic trafficking could be provided (1-4).A technique for monitoring the dynamics of the protein movement inside single living cells relies on the use of immunocytochemistry or optical imaging with genetically tagged GFP (5). These analyses are effective for imaging the spatial and temporal dynamics of proteins of interest within single living cells. For high-throughput analysis of the protein movement inside the cells, automated fluorescence microscopy has been developed (2). Although such technological progress is important, image acquisition can be slow and tedious. In particular, algorithms to automatically determine nuclear vs. cytoplasmic localization in an acquired image still remain imprecise and slow. The obtained results are qualitative rather than quantitative because of the limited number of analyzed cells. In addition, analyses of the protein localization in living animals require complex assay procedures such as extraction of an organ and dividing it into sliced sections, which hampers temporal and quantitative analyses.In our previous studies, a system of split firefly luciferase reconstitution by protein splicing has been developed for detecting protein-protein interactions in cell lines and in living mice, in which the novel use of the new luminescent reporter protein was demonstrated (6, 7). We and others have also reported a split Rluc complementation system for imaging tyrosine phosphorylation and to monitor protein-protein interactions in living cells, where bioluminescence originated from Rluc complementation was sensitively detected (8, 9). A method for identifying mitochondrial proteins by using split GFP reconstitution by protein splicing has recently been developed by our group (10). These studies suggest a great potentiality of Rluc for rapid sensing of intracellular protein movements in living cells and animals.Taking advantage of the bioluminescence of Rluc with background-fluorescence-free and high-sensitive detection, we developed an indicator with general applicability for detecting transport into the nucleus of a particul...

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Toxic Threats to Neurologic Development of Children

Cited by 62 publications

References 24 publications

The Tohoku Study of Child Development: A Cohort Study of Effects of Perinatal Exposures to Methylmercury and Environmentally Persistent Organic Pollutants on Neurobehavioral Development in Japanese Children

The Tohoku Study of Child Development: A Cohort Study of Effects of Perinatal Exposures to Methylmercury and Environmentally Persistent Organic Pollutants on Neurobehavioral Development in Japanese Children

Loss of neuropathy target esterase in mice links organophosphate exposure to hyperactivity

High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of splitRenillaluciferase

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