Toxicity and behavioral effects of dimethylsulfoxide in planaria

Pagán, Oné R.; Rowlands, Amanda L.; Urban, Kimberly R.

doi:10.1016/j.neulet.2006.08.073

Cited by 67 publications

(56 citation statements)

References 28 publications

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“…Dimethylsulfoxide (DMSO) at a concentration of 0.1% was present in all experimental solutions as a solubility-aiding agent for parthenolide. At this concentration, DMSO does not induce any observable behavioral or toxic effects in planarians (Pagán et al, 2006). …”

Section: Methodsmentioning

confidence: 79%

Planarians in pharmacology: parthenolide is a specific behavioral antagonist of cocaine in the planarian Girardia tigrina

Pagán

Baker²,

Deats³

et al. 2012

Int. J. Dev. Biol.

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Planarians are traditional animal models in developmental and regeneration biology. Recently, these organisms are arising as vertebrate-relevant animal models in neuropharmacology. Using an adaptation of published behavioral protocols, we have described the alleviation of cocaine-induced planarian seizure-like movements (pSLM) by a naturally-occurring sesquiterpene lactone, parthenolide. Interestingly, parthenolide does not prevent the expression of pSLM induced by amphetamines; in vertebrates, amphetamines interact with the same protein target as cocaine. Parthenolide is also unable to prevent pSLM elicited by the cholinergic compounds nicotine and cytisine or by the glutamatergic agents L-or D-glutamic acid or NMDA. Thus, we conclude that parthenolide is a specific anti-cocaine agent in this experimental organism.

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Section: Methodsmentioning

confidence: 79%

Planarians in pharmacology: parthenolide is a specific behavioral antagonist of cocaine in the planarian Girardia tigrina

Pagán

Baker²,

Deats³

et al. 2012

Int. J. Dev. Biol.

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“…While soluble in dimethylsulfoxide (DMSO), DMSO itself can cause a locomotion phenotype (Pagan et al, 2006). Therefore, sulpiride solutions were made by adding 0.341g sulpiride powder to 10ml of 10% HCl (Schroeder and Packard, 2000), the pH was adjusted with 1mmoll -1…”

Section: Drug Treatmentmentioning

confidence: 99%

“…To fill this gap, we designed a simple setup that can easily be implemented by any research lab, thus providing a viable alternative to currently used methods such as the planarian locomotor velocity (pLMV) method . The pLMV method, although applied in a number of studies Raffa and Valdez, 2001;Pagan et al, 2006;Rawlsa et al, 2007), only quantifies speed based on the number of gridlines crossed in a given time interval, thereby rendering locomotion phenotypes with reduced mobility indistinguishable. We have executed the pLMV method in this study for wild-type (WT) planarians to allow for a direct comparison with our method.…”

Section: Introductionmentioning

confidence: 99%

Quantitative characterization of planarian wild-type behavior as a platform for screening locomotion phenotypes

Talbot

Schötz

2011

Journal of Experimental Biology

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SUMMARYChanges in animal behavior resulting from genetic or chemical intervention are frequently used for phenotype characterizations. The majority of these studies are qualitative in nature, especially in systems that go beyond the classical model organisms. Here, we introduce a quantitative method to characterize behavior in the freshwater planarian Schmidtea mediterranea. Wild-type locomotion in confinement was quantified using a wide set of parameters, and the influences of intrinsic intra-worm versus interworm variability on our measurements was studied. We also examined the effect of substrate, confinement geometry and the interactions with the boundary on planarian behavior. The method is based on a simple experimental setup, using automated center-of-mass tracking and image analysis, making it an easily implemented alternative to current methods for screening planarian locomotion phenotypes. As a proof of principle, two drug-induced behavioral phenotypes were generated to show the capacity of this method. Supplementary material available online at

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“…56: [183][184][185][186][187][188][189][190][191] doi: 10.1387/ijdb.113485mp www.intjdevbiol.com carcinogenicity, etc. (Kapu and Schaeffer, 1991;Guecheva et al, 2003;Pagan et al, 2006;Li, 2008;Kovacevic et al, 2009;Alonso and Camargo, 2011). They are useful in experimental toxicology as they are easy to manipulate in vivo and make it possible to screen for effects at multiple biological levels.…”

Section: Introductionmentioning

confidence: 99%

Physiological and molecular characterisation of cadmium stress in Schmidtea mediterranea

Plusquin

Stevens²,

Belleghem³

et al. 2012

Int. J. Dev. Biol.

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The planarian Schmidtea mediterranea is a well-studied model organism for developmental research, because of its stem cell system. This characteristic also provides a unique opportunity to study stress management and the effect of stress on stem cells. In this study, we characterised the stress signature at different levels of biological organization. The carcinogenic metal cadmium was used as a model chemical stressor. We focused on stem cell activity and its interaction with other known stress parameters. Here, we have found that S. mediterranea is able to cope with high internal levels of cadmium. At endpoints such as size and mobility, cadmium-related stress effects were detected but all of these responses were transient. Correspondingly, cadmium exposure led to an elevated mitotic activity of the neoblasts, at the same time points when the other responses disappeared. At the molecular level, we observed redox-related responses that can be linked with both repair as well as proliferation mechanisms. Together, our results suggest that these animals have a high plasticity. The induction of stem cell activity may underlie this 'restoring' effect, although a carcinogenic outcome after longer exposure times cannot be excluded.

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Toxicity and behavioral effects of dimethylsulfoxide in planaria

Cited by 67 publications

References 28 publications

Planarians in pharmacology: parthenolide is a specific behavioral antagonist of cocaine in the planarian Girardia tigrina

Planarians in pharmacology: parthenolide is a specific behavioral antagonist of cocaine in the planarian Girardia tigrina

Quantitative characterization of planarian wild-type behavior as a platform for screening locomotion phenotypes

Physiological and molecular characterisation of cadmium stress in Schmidtea mediterranea

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