Toxicity Assessment of Montmorillonite as a Drug Carrier for Pharmaceutical Applications: Yeast and Rats Model

Lee, Yuan-Haun; Kuo, Tzong-Fu; Chen, Bor-Yann; Feng, Yi-Kai; Wen, Yu-Chieh; Lin, Wu-Ching; Lin, Feng-Huei

doi:10.4015/s1016237205000111

Cited by 61 publications

(43 citation statements)

References 2 publications

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“…Lee et al [62] showed that a prolonged 7-day exposure of human dermal fibroblasts to 0.001 % MMT did not lead to significant changes in cell viability. This echoes with the outcome of a study conducted earlier by Lee et al [63] whereby MMT was found to be biologically safe and nontoxic (Saccharomyces cerevisiae (24 h and *10 5 ppm) and Wistar rat (72 h and 142.9 mg/kg BW). As a result, the growth inhibitory effects as observed in the present study were likely the cocontribution from SA and methyl cellulose (MC-0), rather On the contrary, MC-1 and -5 exhibited growth promoting profiles at high concentrations of 100 and 1,000 lg/ ml.…”

Section: Cytotoxicity Profiles Of the Bionanocomposite Films And Theisupporting

confidence: 88%

Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films

Mishra

Ramasamy

Lim

et al. 2014

J Mater Sci: Mater Med

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The present study investigates the development of methyl cellulose (MC)-sodium alginate (SA)-montmorillonite (MMT) clay based bionanocomposite films with interesting wound healing properties. The differential scanning calorimetry analysis of the composite films revealed presence of single glass transition temperature (Tg) confirming the miscible nature of the ternary blended films. The increase in MMT ratio in the composite films reduced the mobility of biopolymer chains (MC/SA) which increased the Tg of the film. Thermogravimetric analysis showed that dispersion of clay (MMT) at nano level significantly delayed the weight loss that correlated with higher thermal stability of the composite films. It was observed that the developed films were able to exhibit antimicrobial activity against four typical pathogenic bacteria found in the presence of wound. The developed films were able to significantly inhibit (10 mg/ml) the growth of Enterococcus faecium and Pseudomonas aeruginosa. In vitro scratch assay indicated potential wound closure activities of MC-2-4 bionanocomposite films at their respective highest subtoxic doses. In conclusion, these ternary bionanocomposite films were found to be promising systems for wound healing applications.

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Section: Cytotoxicity Profiles Of the Bionanocomposite Films And Theisupporting

confidence: 88%

Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films

Mishra

Ramasamy

Lim

et al. 2014

J Mater Sci: Mater Med

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“…SEM images of the crosssection of the explanted nanocomposite showed that fibroblasts were able to easily penetrate and attach to locations within the pore network and consequently produced pore walls with biocolonized surfaces. Although clay nanoparticles can be considered nontoxic 24 , surfactants used to improve the organophilic behavior of clays were reported to be toxic in some studies 26 . In this work, the synthesis of the nanocomposite was designed to enable the formation of chemical bonds between the alkylammonium (surfactant) and the polymer so as to restrict the free diffusion of this surfactant.…”

Section: Discussionmentioning

confidence: 99%

“…Toxicity related to nanoparticles derived from clay minerals is much less known. Some reports have demonstrated that these particles may be considered nontoxic 24,25 , since no histopathological changes could be observed in heart, liver, spleen, lung and kidney of rats after ingestion and intravenous injection of clay nanoparticles, as an indication that they could be eliminated through the kidney system. However, some chemical treatments used to improve the organophilic behavior of the clays may lead to toxicity 26 .…”

Section: Introductionmentioning

confidence: 99%

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

et al. 2010

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A porous biodegradable polyurethane nanocomposite based on poly(caprolactone) (PCL) and nanocomponents derived from montmorillonite (Cloisite ® 30B) was synthesized and tested to produce information regarding its potential use as a scaffold for tissue engineering. Structural and morphological characteristics of this nanocomposite were studied by infrared spectroscopy (FTIR), X-ray diffraction (XRD), small angle X-ray scattering (SAXS) and scanning electron microscopy (SEM). The reaction between polyurethane oligomers with isocyanate endcapped chains and water led to the evolution of CO 2 , which was responsible for building interconnected pores with sizes ranging from 184 to 387 µm. An in vitro cell-nanocomposite interaction study was carried out using neonatal rat calvarial osteoblasts. The ability of cells to proliferate and produce an extracellular matrix in contact with the synthesized material was assessed by an MTT assay, a collagen synthesis analysis, and the expression of alkaline phosphatase. In vivo experiments were performed by subcutaneously implanting samples in the dorsum of rats. The implants were removed after 14, 21, and 29 days, and were analyzed by SEM and optical microscopy after tissue processing. Histology crosssections and SEM analyses showed that the cells were able to penetrate into the material and to attach to many location throughout the pore structure. In vitro and in vivo tests demonstrated the feasibility for polyurethane nanocomposites to be used as artificial extracellular matrices onto which cells can attach, grow, and form new tissues.

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“…Prior to indications of MMT as a good carrier for drug design, Lee et al [6] also examined acute effects of MMT using wistar rats as an in vivo animal model, clearly revealing the harmless nature of MMT. Evidence in biochemical analysis on mice administered with high doses of MMT also indicated that MMT is considered safe for practical use.…”

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

Understanding the Characteristics of L-Ascorbic Acid-Montmorillonite Nanocomposite: Chemical Structure and Biotoxicity

Chen

Lee

Lin

et al. 2006

Biomed. Eng. Appl. Basis Commun.

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Toxicity Assessment of Montmorillonite as a Drug Carrier for Pharmaceutical Applications: Yeast and Rats Model

Abstract: Our previous study indicated that montmorillonite (MMT for short) was pharmaceutically feasible to be used as a carrier of an anticancer drug 5-FU. Emphasis of this study is thus placed on the toxicity of MMT to address whether it is

Cited by 61 publications

References 2 publications

Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films

Antimicrobial and in vitro wound healing properties of novel clay based bionanocomposite films

Porous biodegradable polyurethane nanocomposites: preparation, characterization, and biocompatibility tests

Understanding the Characteristics of L-Ascorbic Acid-Montmorillonite Nanocomposite: Chemical Structure and Biotoxicity

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