Toxicity assessment of saline extract and lectin-rich fraction from Microgramma vacciniifolia rhizome

Silva, Gabriela Cavalcante da; Oliveira, Alisson Macário de; Machado, Janaína Carla Barbosa; Ferreira, Magda Rhayanny Assunção; Medeiros, Paloma Lys de; Soares, Luiz Alberto Lira; Souza, Ivone Antônia de; Paiva, Patrícia Maria Guedes; Napoleão, Thiago Henrique

doi:10.1016/j.toxicon.2020.08.021

Cited by 14 publications

(12 citation statements)

References 55 publications

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“…Thus, both preparations from M. vacciniifolia rhizome were classified as safe with LD 50 >1,000 mg/kg. In comparison with the previous study that evaluated these samples at 2,000 and 5,000 mg/kg, [29] the toxic signs previously observed were not detected on testing a lower dose of 1,000 mg/kg. Thus, the doses selected for evaluating the antinociceptive and anti‐inflammatory activities were below 1,000 mg/kg.…”

Section: Resultscontrasting

confidence: 69%

“…Using the same samples employed in the present work, Silva et al [29] . have reported the presence of cinnamic acid derivatives (0.271±0.0001 g %), including a chlorogenic acid content of 0.135 g %, in the saline extract of M. vacciniifolia rhizome.…”

Section: Resultssupporting

confidence: 55%

“…The anti‐inflammatory properties of the saline extract from M. vacciniifolia could be attributed to the presence of cinnamic derivatives and chlorogenic acid which were previously identified [29] and reported as anti‐inflammatory agents [55,56] . Study evaluating chlorogenic acid‐rich plant species have demonstrated their ability to decrease carrageenan‐induced paw edema in rats [57] .…”

Section: Resultsmentioning

confidence: 98%

“…have reported that the fraction obtained with ammonium sulfate from the saline extract of M. vacciniifolia rhizome is rich in MvRL, with high protein homogeneity. A previous study has evaluated acute toxicity in mice using the saline extract and MvRL‐rich fraction at 2,000 and 5,000 mg/kg, respectively, and reported that no death occurred, but some toxicity signals were observed, including an increase in serum transaminases and urea levels, and the presence of leukocyte infiltrates in the liver and lungs [29] …”

Section: Introductionmentioning

confidence: 99%

“…The reports on the antinociceptive and anti‐inflammatory effects of lectins stimulated us to evaluate these biological activities in the saline extract of M. vacciniifolia rhizome and MvRL‐rich fraction. However, the previous results evidencing some toxicity signs [29] prompted us to prior determine the toxicity of doses lower than 2,000 mg/kg. Thus, in the present study, we first evaluated the acute toxicity of the saline extract and MvRL‐rich fraction at 1,000 mg/kg and then, selected safe doses to evaluate their antinociceptive and anti‐inflammatory activities using different models.…”

Section: Introductionmentioning

confidence: 99%

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Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Silva

Oliveira

Freitas

et al. 2021

Chemistry & Biodiversity

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Previous studies have characterized a saline extract from Microgramma vacciniifolia rhizome and its lectin (MvRL)‐rich fraction with low acute toxicity. In the present study, we evaluated these preparations for acute toxicity (1,000 mg/kg) and antinociceptive and anti‐inflammatory activities (100–400 mg/kg for the extract and 25–50 mg/kg for the fraction). No signs of toxicity were observed. Both the extract and fraction increased the latency period for nociception in the hot plate assay, decreased writhing induced by acetic acid, and promoted analgesic effects in phases 1 and 2 of the formalin test. The antinociceptive mechanism was attributed to interactions with opioid receptors and K+ ATPase channels. The extract and fraction decreased carrageenan‐induced paw edema in 46.15 % and 77.22 %, respectively, at the highest doses evaluated. Furthermore, the fraction was shown to act on the bradykinin pathway. The ability to decrease leukocyte migration after treatment was also verified in the peritonitis and air pouch models. In exudates collected from air pouches, decreased tumor necrosis factor (TNF)‐α and increased interleukin (IL)‐10 levels were noted. Both the extract and fraction also effectively inhibited the development of granulomatous tissue. In conclusion, the substances investigated in this study can be used for the development of novel therapeutic options for pain and inflammatory processes.

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Section: Resultscontrasting

confidence: 69%

Section: Resultssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Silva

Oliveira

Freitas

et al. 2021

Chemistry & Biodiversity

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Documentation of phytochemicals, bio-toxicity and haemato-physiological responses at sub-lethal concentrations of Parthenium hysterophorus hydroethanolic extract in Cyprinus carpio: a concept of waste valorization as a phyto-ichthyotoxin

Khan

Sharma

Sonkar

et al. 2023

Environ Sci Pollut Res

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In the present study, phytoextraction was performed through aqueous, alcoholic and hydroethanolic (80%) solvents followed by phytochemical pro ling and evaluation of median lethal concentration (LC 50 ) of hydroethanolic extract in Common carp, Cyprinus carpio. The haemato-immunological and biochemical response was evaluated at sub-lethal concentrations (T1: 0.395 mg l -1 and T2: 0.79 mg l -1 and control: devoid of extract) at three intervals (24, 48 and 96 h). The study revealed the presence of toxic constituents in extracts namely saponin, rotenone, tannin and alkaloid. Moreover, phytochemical typing exhibited the superior ability of hydroethanolic solvent and the extract was chosen for further biological activities. The anti-bacterial assay revealed the inhibitory capacity, whereas, phyto-haemagglutination revealed clumping of blood cells due to parthenium extract exposure. The haemagglutination limit test revealed the minimum concentration of extract causes agglutination was 1/96 and the haemolytic activity result exhibited clear lysis of sh RBCs. LC 50 of parthenium was found to be 18.99 mg l -1 . The study revealed that signi cant (p < 0.05) reduction in haematoimmunological indices alongside serum biochemical parameters. The results presented in the study emphasise locally available weed plant gajar ghas, P. hysterophorus as a non-chemical phyto-ichthyotoxin.

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Antioxidant and Antileishmanial Activities, and Evaluation of Acute Oral Toxicity and Genotoxicity of Bixa orellana Leaf Extracts

Amorim,

Matoso,

de Barros

et al. 2024

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Toxicity assessment of saline extract and lectin-rich fraction from Microgramma vacciniifolia rhizome

Cited by 14 publications

References 55 publications

Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Antinociceptive and Anti‐Inflammatory Effects of Saline Extract and Lectin‐Rich Fraction from Microgramma vacciniifolia Rhizome in Mice

Documentation of phytochemicals, bio-toxicity and haemato-physiological responses at sub-lethal concentrations of Parthenium hysterophorus hydroethanolic extract in Cyprinus carpio: a concept of waste valorization as a phyto-ichthyotoxin

Antioxidant and Antileishmanial Activities, and Evaluation of Acute Oral Toxicity and Genotoxicity of Bixa orellana Leaf Extracts

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