We investigated the lethal toxicity of Shibukuro and Tama river water near the in ow of Tamagawa hotspring water in Akita Prefecture, Japan. We rst measured heavy metal concentrations in both rivers. We detected iron, arsenic, and aluminum; the concentrations of each tended to decrease from upstream to downstream. We next examined the in uence of river water on zebra sh Danio rerio and water ea Daphnia magna. We observed lethal effects in both species, with Daphnia magna more sensitive to toxicity than Danio rerio. For both species, the toxic effects of river water decreased with increasing distance downstream from the in ow of hot-spring water. Our results show that the heavy metals discharged from Tamagawa hot spring have a negative effect on aquatic organisms.