Toxicity evaluation of iron oxide nanoparticles to freshwater cyanobacteria Nostoc ellipsosporum

Kumar, Mukesh; Seth, Kunal; Choudhary, Sunita; Kumawat, Geetanjali; Nigam, Subhasha; Joshi, Garima; Saharan͙, Vinod; Meena, Mukesh; Gupta, Amit Kumar; Mangesh, Harish

doi:10.1007/s11356-023-26353-2

Cited by 3 publications

(3 citation statements)

References 48 publications

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“…Therefore, the observed increase in extracellular lactate levels in B481-SD treated with nZVIs may be attributed to concentration-related changes in the cell wall and membrane. According to a study by Kumar et al [ 38 ], when the cyanobacterium type Nostoc ellipsosporum (Rabenhorst ex Bornet et Flahault) was treated with FeO nanoparticles at concentrations ranging from 10 to 100 mg/L, an increase in LDH levels consistent with the escalating concentrations was reported, suggesting a dose-dependent response to the nanoparticle treatment.…”

Section: Resultsmentioning

confidence: 99%

Impact of Zero-Valent Iron Nanoparticles and Ampicillin on Adenosine Triphosphate and Lactate Metabolism in the Cyanobacterium Fremyella diplosiphon

Yalcin,

Aydin,

Sitther

2024

Microorganisms

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In cyanobacteria, the interplay of ATP and lactate dynamics underpins cellular energetics; their pronounced shifts in response to zero-valent iron (nZVI) nanoparticles and ampicillin highlight the nuanced metabolic adaptations to environmental challenges. In this study, we investigated the impact of nZVIs and ampicillin on Fremyella diplosiphon cellular energetics as determined by adenosine triphosphate (ATP) content, intracellular and extracellular lactate levels, and their impact on cell morphology as visualized by transmission electron microscopy. While a significant increase in ATP concentration was observed in 0.8 mg/L ampicillin-treated cells compared to the untreated control, a significant decline was noted in cells treated with 3.2 mg/L nZVIs. ATP levels in the combination regimen of 0.8 mg/L ampicillin and 3.2 mg/L nZVIs were significantly elevated (p < 0.05) compared to the 3.2 mg/L nZVI treatment. Intracellular and extracellular lactate levels were significantly higher in 0.8 mg/L ampicillin, 3.2 mg/L nZVIs, and the combination regimen compared to the untreated control; however, extracellular lactate levels were the highest in cells treated with 3.2 mg/L nZVIs. Visualization of morphological changes indicated increased thylakoid membrane stacks and inter-thylakoidal distances in 3.2 mg/L nZVI-treated cells. Our findings demonstrate a complex interplay of nanoparticle and antibiotic-induced responses, highlighting the differential impact of these stressors on F. diplosiphon metabolism and cellular integrity.

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Section: Resultsmentioning

confidence: 99%

Impact of Zero-Valent Iron Nanoparticles and Ampicillin on Adenosine Triphosphate and Lactate Metabolism in the Cyanobacterium Fremyella diplosiphon

Yalcin,

Aydin,

Sitther

2024

Microorganisms

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“…For example, Leptolyngbya valderiana (Banerjee et al, 2021), Leptolyngbya sp. WUC 59 (Singh et al, 2020), Nostoc ellipsosporum (Kumar et al, 2023), Anabaena flos‐aquae , and Calothrix pulvinata (Brayner et al, 2012) were employed for the biogenic synthesis of NPs. These studies demonstrated that the biological methods are cost‐effective, environmentally friendly, with a wide range of biomedical applications (Hamida et al, 2020; Hamouda et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Anabaena sp. A‐1 mediated molybdenum oxide nanoparticles: A novel frontier in green synthesis, characterization and pharmaceutical properties

Malik,

Minhas,

Hassan

et al. 2024

Microscopy Res & Technique

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Green‐synthesized metal oxide nanoparticles have garnered considerable attention due to their simple, sustainable, and eco‐friendly attributes, coupled with their diverse applications in biomedicine and environmental context. The current study shows a sustainable approach for synthesizing molybdenum oxide nanoparticles (MoONPs) utilizing an extract from Anabaena sp. A‐1. This novel approach marks a significant milestone as various spectral approaches were employed for characterization of the green‐synthesized MoONPs. Ultraviolet–visible (UV–Vis) spectroscopic analysis revealed a surface plasmon resonance (SPR) peak of MoONPs at 538 nm. Fourier transform infrared (FTIR) spectral analysis facilitated the identification of functional groups responsible for both the stability and production of MoONPs. Scanning electron microscopy (SEM) was utilized revealing a rod shape morphology of the MoONPs. X‐ray diffraction (XRD) analysis yielded a calculated crystal size of 31 nm, indicating the crystalline nature of MoONPs. Subsequently, biological assays were employed to ascertain the potential of the bioengineered MoONPs. The 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) assay was used to quantify free radical scavenging activity, revealing an antioxidant capacity of 68.1% at 200 μg/mL. To evaluate antibacterial and antifungal efficacy, the disc diffusion method was employed across varying concentrations of MoONPs (6.25, 12.5, 25, 50, 100, 150, 200 μg/mL). Quantification of cytotoxicity was performed via a brine shrimp assay, yielding an IC50 value of 552.3 μg/mL, a metric of moderate cytotoxicity. To assess the biocompatibility of MoONPs, an antihemolytic assay was conducted, confirming their safety profile. Additionally, MoONPs exhibited non‐toxic attributes in an insecticidal assay. Notably, in anti‐inflammatory assay MoONPs showed an inactive nature towards the reactive oxygen species. In conclusion, these findings highlight the potential versatility of MoONPs in various biological applications, extending beyond their recognized anti‐inflammatory and insecticidal properties.Research Highlights This study marks an advancement in nanotechnology, exploring ways for MoONPs fabrication, representing a unique and unexplored research domain. Green‐synthesized MoONPs using Anabaena sp. A‐1 extract offers a sustainable and eco‐friendly approach. Characterized by UV–Vis, FTIR, SEM, and XRD, MoONPs demonstrate rod‐shaped morphology and crystalline nature. Bioengineered MoONPs exhibit versatility in biological applications, demonstrating notable antioxidant, antibacterial and antifungal efficacy, moderate cytotoxicity, biocompatibility, and insecticidal properties, emphasizing their multifaceted utility. The research findings highlight the potential utilization of MoONPs across a spectrum of biological applications, thereby suggesting their promising role in the realm of biomedicine and environmental context.

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“…For instance, cyanobacteria Leptolyngbya valderiana [ 29 ], Leptolyngbya sp. WUC 59 [ 30 ], Nostoc ellipsosporum [ 31 ], Anabaena flos-aquae and Calothrix pulvinata [ 32 ] Oscillatoria limnetica [ 33 ] were employed for the biogenic synthesis of FeONPs. Oscillatoria limnetica and Desertifilum sp.…”

Section: Introductionmentioning

confidence: 99%

Biogenic Fabrication of Iron Oxide Nanoparticles from Leptolyngbya sp. L-2 and Multiple In Vitro Pharmacogenetic Properties

Minhas,

Kaleem,

Minhas

et al. 2023

Toxics

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Metallic nanoparticles have received a significant amount of reflection over a period of time, attributed to their electronic, specific surface area, and surface atom properties. The biogenic synthesis of iron oxide nanoparticles (FeONPs) is demonstrated in this study. The green synthesis of metallic nanoparticles (NPs) is acquiring considerable attention due to its environmental and economic superiorities over other methods. Leptolyngbya sp. L-2 extract was employed as a reducing agent, and iron chloride hexahydrate (FeCl3·6H2O) was used as a substrate for the biogenic synthesis of FeONPs. Different spectral methods were used for the characterization of the biosynthesized FeONPs, ultraviolet-visible (UV-Vis) spectroscopy gave a surface plasmon resonance (SPR) peak of FeONPs at 300 nm; Fourier transform infrared (FTIR) spectral analysis was conducted to identify the functional groups responsible for both the stability and synthesis of FeONPs. The morphology of the FeONPs was investigated using scanning electron microscopy (SEM), which shows a nearly spherical shape, and an X-ray diffraction (XRD) study demonstrated their crystalline nature with a calculated crystallinity size of 23 nm. The zeta potential (ZP) and dynamic light scattering (DLS) measurements of FeONPs revealed values of −8.50 mV, suggesting appropriate physical stability. Comprehensive in-vitro pharmacogenetic properties revealed that FeONPs have significant therapeutic potential. FeONPs have been reported to have potential antibacterial and antifungal properties. Dose-dependent cytotoxic activity was shown against Leishmania tropica promastigotes (IC50: 10.73 µg/mL) and amastigotes (IC50: 16.98 µg/mL) using various concentrations of FeONPs. The cytotoxic potential was also investigated using brine shrimps, and their IC50 value was determined to be 34.19 µg/mL. FeONPs showed significant antioxidant results (DPPH: 54.7%, TRP: 49.2%, TAC: 44.5%), protein kinase (IC50: 96.23 µg/mL), and alpha amylase (IC50: 3745 µg/mL). The biosafety of FeONPs was validated by biocompatibility tests using macrophages (IC50: 918.1 µg/mL) and red blood cells (IC50: 2921 µg/mL). In conclusion, biogenic FeONPs have shown potential biomedical properties and should be the focus of more studies to increase their nano-pharmacological significance for biological applications.

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Toxicity evaluation of iron oxide nanoparticles to freshwater cyanobacteria Nostoc ellipsosporum

Cited by 3 publications

References 48 publications

Impact of Zero-Valent Iron Nanoparticles and Ampicillin on Adenosine Triphosphate and Lactate Metabolism in the Cyanobacterium Fremyella diplosiphon

Impact of Zero-Valent Iron Nanoparticles and Ampicillin on Adenosine Triphosphate and Lactate Metabolism in the Cyanobacterium Fremyella diplosiphon

Anabaena sp. A‐1 mediated molybdenum oxide nanoparticles: A novel frontier in green synthesis, characterization and pharmaceutical properties

Biogenic Fabrication of Iron Oxide Nanoparticles from Leptolyngbya sp. L-2 and Multiple In Vitro Pharmacogenetic Properties

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