Toxicity evaluation of the contaminated area of Crotone from biological indicators: a multispecies approach

Abstract

Contamination of terrestrial and aquatic ecosystems by toxic industrial waste has become a major issue in many countries. Of particular concern is the reuse of toxic hazardous waste in construction materials. This paper examined for the first time the chemical and radiation ecotoxicity of site-specific Technological Enhanced Naturally Occurring Radioactive Materials (TENORM) residues from phosphate processing industry in soil environmental matrices through bioindicators. The area under investigation was the former industrial district of Crotone (Calabria, Italy), recently included within the Sites of National Interest (SIN), comprising the 42 Italian national priority contaminated sites. Major biological exposure pathways considered were absorption and bioaccumulation. The marine bacterium Vibrio fischeri and the freshwater crustacean Daphnia magna were employed as aquatic bioindicators, while for the soil ecosystem the seeds of Sorghum saccharatum and Lepidium sativum were used. The selection of test species aimed at assessing the toxicity of wastes in soil as well as in freshwater or marine systems. Results indicated Vibrio fischeri as the most sensitive of all the species tested (5.56 g/L), while Daphnia magna was found to be affected at 94.27 g/L. An overall inhibition was observed in seedling growth as compared to control at the highest concentration of the pollutants (100 g/L), while seed germination was not adversely affected by the pollutant. At this preliminary level, data indicated a potential risk for biodiversity of the area. In fact, the measured toxicity thresholds, even below 100 mg/L, are comparable to concentrations of the toxicants spread all over the territory of Crotone.