Toxicity monitoring of the Scheldt and Meuse rivers in Wallonia (Belgium) by conventional tests and microbiotests

Wielen, Claire Van der; Halleux, Isabelle

doi:10.1007/978-1-4615-4289-6_34

Cited by 5 publications

(2 citation statements)

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“…Neither of the test protocols revealed any toxicity for samples 38 and 71, which originated from a usually contaminated sampling point in the toxicological monitoring programme. This location was recognised as toxic by the Microtox system, as well as by other bioassays (8).…”

Section: Resultsmentioning

confidence: 86%

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Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox^® Method

2000

Altern Lab Anim

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A previously described neutral red uptake-inhibition assay on cultured fathead minnow (FHM) fish cells revealed a good correlation with fish toxicity data. The assay was made more sensitive by reducing the number of cells, by using a longer treatment period, and by simultaneous treatment of the cells with sodium dodecyl sulphate and L-buthionine (S,R)-sulphoximine. The fluorimetrically quantified protein content was then used as the endpoint. The results were compared with toxicity data obtained by the Microtox® method with the bacterium, Vibrio fischeri. A series of 82 surface-water samples were investigated. Cell quantity-dependent and sample concentration-dependent reductions in total protein content were observed. In all, 32 samples were toxic in the FHM assay. Of the seven samples that were shown to be toxic in the Microtox assay, only three were also toxic in FHM cells. No linear relationship was found for the toxicity results obtained with the two methods. Further research to explain the significance of the many positive responses and to identify possible confounding factors will increase the reliability of the sensitised FHM cell assay.

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Section: Resultsmentioning

confidence: 86%

“…The Microtox results were obtained within the ecotoxicological monitoring programme of surface waters in Wallonia, Belgium (8). The method is based on the inhibition of the luminescence of V. fischeri (previously known as Photobacterium phosphoreum).…”

Section: Methodsmentioning

confidence: 99%

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox^® Method

2000

Altern Lab Anim

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Screening for antibacterial activity in 72 species of wood‐colonizing fungi by the Vibrio fisheri bioluminescence method

Berden-Zrimec

Zrimec²,

Slanc

et al. 2004

J. Basic Microbiol.

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Resistance of pathogenic bacteria to antibiotics leads scientists to discover new antibacterial drugs. Ninety samples of wood-colonizing fungi were cultivated on agar plates, and their extracts tested for antibacterial activity using the Vibrio fischeri bioluminescence test. Two fungi species, Serpula lacrymans and Nectria vilior, were found to be a potential new source of thermostable antibiotics. Vibrio fischeri bioluminescence test was found to be a useful method for antibacterial activity screening from the samples of natural origin.

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Overview of Contemporary Toxicity Testing

Blaise

Férard²

Small-Scale Freshwater Toxicity Investigations

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Toxicity monitoring of the Scheldt and Meuse rivers in Wallonia (Belgium) by conventional tests and microbiotests

Cited by 5 publications

References 1 publication

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox^® Method

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox^® Method

Screening for antibacterial activity in 72 species of wood‐colonizing fungi by the Vibrio fisheri bioluminescence method

Overview of Contemporary Toxicity Testing

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Toxicity monitoring of the Scheldt and Meuse rivers in Wallonia (Belgium) by conventional tests and microbiotests

Cited by 5 publications

References 1 publication

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox® Method

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox® Method

Screening for antibacterial activity in 72 species of wood‐colonizing fungi by the Vibrio fisheri bioluminescence method

Overview of Contemporary Toxicity Testing

Contact Info

Product

Resources

About

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox^® Method

Toxicological Evaluation of Surface-water Samples in Sensitised Cultured Fish Cells Compared with the Microtox^® Method