Toxicity of 20 Chemicals from the MEIC Programme Determined by Growth Inhibition of L-929 Fibroblast-like Cells

Järkelid, Lena; Kjellstrand, Per; Martinson, E. E.; Wieslander, Anders

doi:10.1177/026119299702500108

Cited by 4 publications

(1 citation statement)

References 8 publications

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“…To date, very few experiments have been performed with fibroblast cells to test the wound-healing activity of drugs, although fibroblast cells have been widely used in a variety of biological tests; for example, studies for anti-ageing activity (34-37), toxicity testing (38,39) and free-radical activity (40). A previous study has shown that pantothenic acid formed by the metabolism of dexpanthenol increases the mitotic activity of cultured human fibroblasts (41).…”

Section: Discussionmentioning

confidence: 99%

Testing Wound-healing Activity in T15 Fibroblast Cultures: A Morphometric Analysis

Korkmaz

Zeytinoğlu²,

Zeytinoğlu³

et al. 2000

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The purpose was to evaluate the use of mouse T15 fibroblast cell cultures for the investigation of wound-healing activity. In order to investigate their mechanisms of action, the effects of drugs with wound-healing activities were compared by using morphometric analyses by microscopy after cell staining. A number of parameters were used to evaluate the effects of titrated extracts from Centella asiatica and dexpanthenol (drugs that have been used in medical practice for their wound-healing activities) on cultured mouse T15 fibroblasts. These parameters were: the total number of cells; the number of T15 cells in mitosis; the percentages of fusiform, polygonal, round and vacuole-containing cells; and the number of intracellular collagen granules. The results indicate that these two drugs exhibit wound-healing activities by activating fibroblast cells, and have cytoprotective effects, although their mechanisms of action on mouse T15 fibroblasts were different. On the basis of our findings, mouse T15 fibroblast cell cultures seem to be useful for the pharmacological screening of compounds with wound-healing activity.

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Section: Discussionmentioning

confidence: 99%

Testing Wound-healing Activity in T15 Fibroblast Cultures: A Morphometric Analysis

Korkmaz

Zeytinoğlu²,

Zeytinoğlu³

et al. 2000

Altern Lab Anim

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Acute cytotoxicity testing with cultured human lung and dermal cells

Barile

Cardona

1998

In Vitro Cell.Dev.Biol.-Animal

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An extensive in vitro study with cultured cells was conducted to test the basal cytotoxicity theory. This theory suggests that most chemical injury, at least in vitro, is a manifestation of one or more insults to the basic cellular structures and functions common to mammalian cells. This accounts for the similarity of results in multilaboratory studies. Human fetal lung fibroblasts (HFL1), and human skin fibroblasts (WS1, Detroit551) were studied in culture to evaluate their potential to screen for cytotoxicity. Confluent monolayers were incubated in the absence or presence of increasing concentrations of test chemicals for 24 h, and the MTT assay was used to assess toxicity. Inhibitory concentrations were extrapolated from concentration-effect curves after linear regression analysis. Twenty-nine chemicals were tested with each cell line and the cytotoxicity data compared to rodent and human lethal concentrations. The data suggest that the experimental IC50 values are as accurate predictors of human toxicity as equivalent toxic blood concentrations derived from rodent LD50s. In addition, lung and skin fibroblasts revealed no significant differences among the three cell lines. The results support the conclusion that finite cell lines of human origin have the potential for screening chemicals for human toxicity. In combination with previously published reports, the data suggest that a basal cytotoxic phenomenon may explain the similarity of results among different human cell lines.

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