Toxicity of boron and vanadium nanoparticles on Danio rerio embryos – Phenotypical, biochemical, and behavioral alterations

Santos, Joana; Barreto, Ângela; Almeida, C.M.V.B.; Azevedo, Cátia; Domingues, Inês; Amorim, Mónica J.B.; Maria, V.L.

doi:10.1016/j.aquatox.2021.105930

Cited by 13 publications

(7 citation statements)

References 74 publications

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“…However, innovative nanomaterials could also bring about certain risks and hazards to human health and nature, so ecotoxicity studies are of paramount importance in assessing such potential detrimental effects of NPs (Almeida et al, 2019;Haque & Ward, 2018). It is known that NPs can be easily released into nitride nanotubes [BNNTs], and borax) in order to investigate their cytotoxic (Ciaravino et al, 2013;Hadrup et al, 2021;NTP, 1987;O'Donovan et al, 2011), genotoxic (Turkez et al, 2012), antigenotoxic (Demir & Marcos, 2018;Sarıkaya et al, 2016), and their hazardous effects on survival, development, and behavior (Hadrup et al, 2021;Santos et al, 2021), there has been no research to focus only on the biological effects of B 2 O 3 in non-target organisms.…”

Section: Discussionmentioning

confidence: 99%

“…Even though a number of in vitro and in vivo studies have been carried out with boron‐containing materials (boron, boric acid, boron nitride nanotubes [BNNTs], and borax) in order to investigate their cytotoxic (Ciaravino et al, 2013; Hadrup et al, 2021; NTP, 1987; O'Donovan et al, 2011), genotoxic (Turkez et al, 2012), antigenotoxic (Demir & Marcos, 2018; Sarıkaya et al, 2016), and their hazardous effects on survival, development, and behavior (Hadrup et al, 2021; Santos et al, 2021), there has been no research to focus only on the biological effects of B 2 O 3 in non‐target organisms. There is just one study focused on the effects of B 2 O 3 in colon cancer cells in the literature (Albuz et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, the only ecotoxicity study in the literature reported that after 48 hours of acute exposure to BNPs at a concentration of 6.7 mg/L, half (LC 50 ) of the D. magna population used in experiments died, which suggested that this species might be more sensitive to such lethal doses as compared to the zebrafish (Danio rerio)(Strigul et al, 2009). Besides, the teratogenic effects of BNPs in D. rerio have recently been reported by research carried out bySantos et al (2021), who concluded that NP forms of boron compounds may be more toxic than those in ionic or element forms. Similarly, DNA damage effects were slightly more pronounced than the nano form of B 2 O 3 in the current study.…”

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confidence: 99%

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Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Demir

2022

J of Applied Toxicology

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Boron trioxide nanoparticles (B 2 O 3 NPs) have recently been widely used in a range of applications including electronic device technologies, acousto-optic apparatus fields, and as nanopowder for the production of special glasses. We propose Drosophila melanogaster as a useful in vivo model system to study the genotoxic risks associated with NP exposure. In this study, we have conducted a genotoxic evaluation of B 2 O 3 NPs (size average 55.52 ± 1.41 nm) and its ionic form in D. melanogaster. B 2 O 3 NPs were supplied to third instar larvae at concentrations ranging from 0.1-10 mM. Toxicity, intracellular oxidative stress (reactive oxygen species, ROS), phenotypic alterations, genotoxic effect (via the wing somatic mutation and recombination test, SMART), and DNA damage (via Comet assay) were the end-points evaluated. B 2 O 3 NPs did not cause any mutagenic/recombinogenic effects in all tested non-toxic concentrations in Drosophila SMART. Negative data were also obtained with the ionic form. Exposure to B 2 O 3 NPs and its ionic form (at two highest concentrations, 2.5 and 5 mM) was found to induce DNA damage in Comet assay. Additionally, ROS induction in hemocytes and phenotypic alterations were determined in the mouths and legs of Drosophila. This study is the first study reporting genotoxicity data in the somatic cells of Drosophila larvae, emphasizing the importance of D. melanogaster as a model organism in investigating the different biological effects in a concentrationdependent manner caused by B 2 O 3 NPs and its ionic form. The obtained in vivo results contribute to improvement the genotoxicity database on the B 2 O 3 NPs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Demir

2022

J of Applied Toxicology

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“…19 The group led by Maria investigated the toxicity of boron nanoparticles in zebrafish, revealing their biocompatibility up to 10 ppm but revealing minor malformation at the same dosage. 20 However, in vivo toxicity analysis for borondoped CDs remains unexplored, underscoring the need for further investigation, given their potential in biomedical applications, specifically in diagnostic and therapeutic applications.…”

Section: ■ Introductionmentioning

confidence: 99%

“…While in vitro cytotoxicity analysis for CDs generally indicates low cytotoxicity, limited in vivo toxicity studies have been conducted, suggesting safe concentrations below 200 μg/mL in zebrafish . The group led by Maria investigated the toxicity of boron nanoparticles in zebrafish, revealing their biocompatibility up to 10 ppm but revealing minor malformation at the same dosage . However, in vivo toxicity analysis for boron-doped CDs remains unexplored, underscoring the need for further investigation, given their potential in biomedical applications, specifically in diagnostic and therapeutic applications.…”

Section: Introductionmentioning

confidence: 99%

Strategic Assessment of Boron-Enriched Carbon Dots/Naproxen: Diagnostic, Toxicity, and In Vivo Therapeutic Evaluation

Wibrianto,

Putri,

Nisa

et al. 2024

Mol. Pharmaceutics

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Cancer is a significant global public health concern, ranking as the leading cause of mortality worldwide. This study thoroughly explores boron-doped carbon dots (B-CDs) through a simple/rapid microwave-assisted approach and their versatile applications in cancer therapy. The result was highly uniform particles with an average diameter of approximately 4 nm. B-CDs exhibited notable properties, including strong fluorescence with a quantum yield of 33%. Colloid stability tests revealed their robustness within a pH range of 6−12, NaCl concentrations up to 0.5 M, and temperatures ranging from 30 to 60 °C. The study also delved into the kinetics of naproxen release from B-CDs as a drug delivery system. The loading efficacy of naproxen exceeded 55.56%. Under varying pH conditions, the release of naproxen from B-CDs conformed to the Peppas−Sahlin model, demonstrating the potential of Naproxen-loaded CDs for cancer drug delivery. In vitro cytotoxicity assessments, conducted using the CCK-8 Assay and flow cytometry, consistently indicated low toxicity with average cell viability exceeding 80%. An in vivo toxicity test on female mice administered 20 mg/kg of B-CDs for 31 days revealed reversible histological changes in the liver and kidneys, while the pancreas remained unaffected. Importantly, B-CDs did not impact the mice's physical behavior, body weight, or survival. In vivo experiments targeting benzo(a)pyrene-induced fibrosarcoma demonstrated the efficacy of B-CDs as naproxen carriers in the treatment of cancer. This in vivo study provides a thorough comprehension of B-CDs synthesis and toxicity and their potential applications in cancer therapy and drug delivery systems.

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Effectiveness of bimetallic ZnO-B2O3 nanoparticles produced by Streptomyces gancidicus as prospective antifungal agents and therapeutic nutrients to enhance pea plant immunity against damping off-causing Pythium irregulare: in vivo and in vitro investigations

Elkhodary,

Attia,

El-Sayyad

et al. 2023

Biomass Conv. Bioref.

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Pythium irregulare (P. irregulare) is one of the soil-borne pathogens that is the primary cause of damage to several plants each year. The novelty and originality of this work were the ability of Streptomyces gancidicus (S. gancidicus OR229936) to synthesize bimetallic zinc oxide-boron oxide nanoparticles (ZnO-B2O3 NPs) for reducing P. irregulare growth and safeguarding pea plant from damping off disease. The produced bimetallic ZnO-B2O3 NPs’ XRD results highlighted the ZnO diffraction peaks at 2Ɵ = 27.50°, 31.15°, 45.15°, 56.89°, 67.98°, and 75.25°, which are complemented by the standard card JCPDS number 361451 and correspond to (002), (101), (102), (110), (103), and (201) Bragg’s reflections. Along with the standard card JCPDS number 300019, they additionally include the B2O3 NP diffraction peaks at 2Ɵ = 15.25°, 28.69°, 31.99°, and 41.28°. Bimetallic ZnO-B2O3 NPs were tested against P. irregular for their antifungal activities. The findings indicated that ZnO-B2O3 NPs exhibited potential anti P. irregulare activity, with an inhibition zone of 33 mm at a concentration of 1000 µg/mL and a promising MIC of 0.01 µg/mL. Bimetallic ZnO-B2O3 NPs (0.01 ppm) application appeared to significantly lessen the severity of the pea post-emergence damaging off disease by 10% and to provide significant protection by 88%. In comparison to fungicide (difenoconazole 25%) treatments, all metabolic resistance indicators significantly enhanced after the usage of bimetallic ZnO-B2O3 NPs, ZnO NPs, and B2O3 NPs with ethyl acetate extract of S. gancidicus. The beneficial impacts of the bimetallic ZnO-B2O3 NPs, ZnO NPs, and B2O3 NPs have been broadened to increase the enzyme activities of peroxidase (POD) and polyphenol oxidase (PPO) in both healthy and infected pea plant in comparison to control. Reduction of Malondialdehyde content (MDA) in response to S. gancidius filtrate, bimetallic ZnO-B2O3 NPs, ZnO NPs, B2O3 NPs, and difenoconazole by 41.68%, 36.51%, 26.15, 26.15, and 15.25%, respectively. Also, contents of H2O2 in infected pea plant were diminished by 50%, 45%, 40%, 37.5%, and 22.5% at bimetallic ZnO-B2O3 NPs, S. gancidicus filtrate, ZnO NPs, difenoconazole, and B2O3 NPs comparing to P. irregular-infected pea plant is strong evidence to induce disease recovery. The application of bimetallic ZnO-B2O3 NPs seems to be a significant approach to relieve the toxic influences of P. irregulare on infected pea plant as green and alternative therapeutic nutrients of chemical fungicides.

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Toxicity of boron and vanadium nanoparticles on Danio rerio embryos – Phenotypical, biochemical, and behavioral alterations

Cited by 13 publications

References 74 publications

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Exposure to boron trioxide nanoparticles and ions cause oxidative stress, DNA damage, and phenotypic alterations in Drosophila melanogaster as an in vivo model

Strategic Assessment of Boron-Enriched Carbon Dots/Naproxen: Diagnostic, Toxicity, and In Vivo Therapeutic Evaluation

Effectiveness of bimetallic ZnO-B2O3 nanoparticles produced by Streptomyces gancidicus as prospective antifungal agents and therapeutic nutrients to enhance pea plant immunity against damping off-causing Pythium irregulare: in vivo and in vitro investigations

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