Toxicity of cadmium, endosulfan, and atrazine in adrenal steroidogenic cells of two amphibian species, Xenopus laevis and Rana catesbeiana

Abstract

The effects of cadmium, endosulfan, and atrazine on corticosterone secretion and viability of adrenal cells of African clawed frog (Xenopus laevis) and bullfrog (Rana catesbeiana) were assessed in vitro using a new bioassay. The bioassay relies on stimulation with adrenocorticotropic hormone (ACTH), the endogenous secretagogue for corticosterone secretion, and with dibutyryl cyclic adenosine monophosphate (dbcAMP), an analogue of cAMP, to pinpoint the site of action of the xenobiotics within the steroidogenic cell. To compare the test toxicants according to their endocrine-disrupting potential, the lethal concentration needed to kill 50% of the cells:effective concentration of 50% (LC50:EC50) ratio was calculated, with LC50 as the concentration that kills 50% of the steroidogenic cells and the EC50 as the concentration that impairs corticosterone secretion by 50%. The higher the ratio, the higher the potential for endocrine disruption. Atrazine had no affect on cell viability and on corticosterone secretion in X. laevis, but its endocrine-disrupting potential was high in R. catesbeiana. The LC50:EC50 ratio for cadmium and endosulfan in X. laevis was 26.07 and 1.23, respectively, and for atrazine, cadmium, and endosulfan in R. catesbeiana it was 909, 41, and 3, respectively. The dbcAMP did not restore corticosterone secretion in the cells exposed to the test toxicants in both species. Our study suggests that the secretory capacity of adrenal cells of amphibians can be impaired by environmental chemicals, especially atrazine in the bullfrog, and that these adrenotoxicants disrupt the enzymatic pathways leading to corticosterone secretion downstream from the step-generating cAMP.

Review and Meta-analysis of Risk Estimates for Prostate Cancer in Pesticide Manufacturing Workers

“…Several pesticides may interfere with sexual hormones through direct action on receptors but also through indirect non-receptorial mechanisms (e.g. organochlorines: for review, see [99,113]; triazines: for review, see [114][115][116][117][118][119][120][121][122][123]; for 2,4-D: [124][125][126]. For other pesticides, it is reported that mutagenicity and alterations of other cellular processes including protein metabolism and cell proliferation may contribute to their clastogenic and tumorigenic activity (chloroacetanilides: [127]; halogenated hydrocarbon nematocide-DBCP: [128][129][130][131][132].…”

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“…They were collected using hand nets to prevent injury to animals during capture since they are active animals. Acclimation to laboratory conditions was done for two weeks prior to experiments (Goulet and Hontella, 2003) in plastic tanks measuring 49 cm in length x 29 cm in width x 24 cm in height with dechlorinated tap water (2 litres at a slant). The frogs were fed ad libitum daily with termites.…”

Effects of environmentally relevant concentrations of endosulfan, azinphosmethyl, and diazinon on Great Basin spadefoot (Spea intermontana) and Pacific treefrog (Pseudacris regilla)

“…Unhatched embryos seem to be less sensitive to xenobiotic chemicals in the laboratory, as numerous experiments have resulted in delayed mortalities in jellied embryos when compared with non-jellied embryos [1,12,21,[24][25][26]. This is likely due to slower water uptake and lower clearance rate in the case of the jellied embryos [22,23].…”