Toxicity of copper in natural marine picoplankton populations

Debelius, Bibiana; Forja, Jesús M.; DelValls, T.À.; Lubián, Luís M.

doi:10.1007/s10646-009-0377-3

Cited by 27 publications

(22 citation statements)

References 28 publications

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“…Seventy-two-hour toxicity tests were performed in two batches: coastal samples were exposed to lower copper concentrations (preliminary studies showed high sensitivity to copper; Debelius et al 2009) of 0, 1.5, 3 and 9 µg L -1 and oceanic samples were exposed to higher copper concentrations of 0, 3, 9 and 18 µg L -1 , all performed in triplicate. The different copper concentrations were added as diluted Merck standard solution; pH was measured at the beginning and end of the toxicity tests, with no variations being found (added acidified stock was less than 0.5% of the total volume).…”

Section: Toxicity Assaysmentioning

confidence: 99%

Toxic effect of copper on marine picophytoplankton populations isolated from different geographic locations

Debelius¹,

Forja²,

DelValls³

et al. 2010

Sci. Mar.

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SUMMARY:In an approach to understand more about copper toxicity effects on marine microalgae, 72-h growth inhibition toxicity tests were followed on natural picophytoplanktonic populations belonging to different coastal and oceanic geographic locations (Mediterranean Sea and Atlantic Ocean) covering, at an approximate longitude of 30ºW, latitudes from 32º14´S to 29º10´N. Analyses of samples established different predominant populations for different locations. Copper toxicity tests showed a group of cyanobacteria isolated from coastal samples that were the most sensitive, and a second group of picoeukaryotes collected from the Atlantic Ocean surface, which were more tolerant to copper. The use of flow cytometry for studying copper toxic effects provided a variety of information on cell parameters in addition to cellular density. In particular, an established increase in copper tolerance with increase of cell size was observed in cyanobacteria, but it was not clearly followed in picoeukaryotes. This study aims to relate copper tolerance to the geographic location sites of sampling collection. The results obtained establish that other factors such as cell size, proximity to the coast and copper concentration in situ are considered to have a greater influence than the fact of belonging to a determined location site.Keywords: natural picoplankton populations, geographic distribution, copper, toxicity, cyanobacteria, picoeukaryotes. RESUMEN: Efecto tóxico del cobre en poblaciones naturales de picoplancton aisladas, procedentes de distintas localizaciones geográficas. -Con el fin de estudiar el efecto tóxico del cobre en las microalgas marinas, se llevaron a cabo ensayos de toxicidad (72 h) con poblaciones naturales de picofitoplancton. Poblaciones procedentes de distintas localizaciones geográficas (mar Mediterráneo y océano Atlántico), cubriendo, aproximadamente en la longitud de 30ºO, latitudes comprendidas entre 32º14´S y 29º10´N. El análisis inicial de las muestras permitió distinguir las poblaciones predominantes de cada estación de muestreo, a exponer en los ensayos de toxicidad. En resumen, un grupo de cianobacterias procedentes de las muestras costeras resultaron ser las más sensibles, y un segundo grupo de picoeucariotas, tomadas de la superficie del océano Atlántico, las más tolerantes al cobre. El análisis del ensayo se llevó a cabo mediante citometría de flujo lo que permitió estudiar otros parámetros celulares, aparte de la densidad celular. Pudiendo observar, en el caso de las cianobacterias, un incremento de la tolerancia al cobre con incremento de su tamaño celular; que no resultó tan evidente en el caso del grupo de picoeucariotas. Finalmente, al relacionar la tolerancia al cobre con las poblaciones procedentes de distintas localizaciones geográficas de muestreo podemos concluir que, otros factores, tales como: el tamaño celular, proximidad a costa, o concentraciones del metal in situ, pueden tener una mayor influencia que la localización geográfica de la población.

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Section: Toxicity Assaysmentioning

confidence: 99%

Toxic effect of copper on marine picophytoplankton populations isolated from different geographic locations

Debelius¹,

Forja²,

DelValls³

et al. 2010

Sci. Mar.

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“…as an example, toxicological research demonstrates that ionized (+2) copper is toxic to bacteria, fungi, microbes, and other simple life forms at low concentrations (Debelius et al, 2009;El-Gendy, Radwan, and Gad, 2009;Serra and Guasch, 2009). At the same time, it is also known that other species require low levels of ionized copper as an essential element of their diet to ensure normal biological functioning (Chen and Chan, 2009).…”

Section: Environmental Issues and Toxicologymentioning

confidence: 99%

Exploring green criminology: toward a green criminological revolution

2014

Choice Reviews Online

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“…Within Synechococcus, clades or strains also have variable Cu tolerances and Cu quotas, which may influence their distribution both with geographic location and depth (Debelius et al, 2009;Stuart et al, 2009;Guo et al, 2012). Despite this evidence that metal toxicity and stress response are important in shaping Synechococcus ecophysiology, how metal toxicity shapes Synechococcus community structure is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Copper toxicity response influences mesotrophic Synechococcus community structure

Stuart

Bundy

Buck

et al. 2017

Environmental Microbiology

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Picocyanobacteria from the genus Synechococcus are ubiquitous in ocean waters. Their phylogenetic and genomic diversity suggests ecological niche differentiation, but the selective forces influencing this are not well defined. Marine picocyanobacteria are sensitive to Cu toxicity, so adaptations to this stress could represent a selective force within, and between, 'species', also known as clades. Here, we compared Cu stress responses in cultures and natural populations of marine Synechococcus from two co-occurring major mesotrophic clades (I and IV). Using custom microarrays and proteomics to characterize expression responses to Cu in the lab and field, we found evidence for a general stress regulon in marine Synechococcus. However, the two clades also exhibited distinct responses to copper. The Clade I representative induced expression of genomic island genes in cultures and Southern California Bight populations, while the Clade IV representative downregulated Fe-limitation proteins. Copper incubation experiments suggest that Clade IV populations may harbour stress-tolerant subgroups, and thus fitness tradeoffs may govern Cu-tolerant strain distributions. This work demonstrates that Synechococcus has distinct adaptive strategies to deal with Cu toxicity at both the clade and subclade level, implying that metal toxicity and stress response adaptations represent an important selective force for influencing diversity within marine Synechococcus populations.

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Toxicity of copper in natural marine picoplankton populations

Cited by 27 publications

References 28 publications

Toxic effect of copper on marine picophytoplankton populations isolated from different geographic locations

Toxic effect of copper on marine picophytoplankton populations isolated from different geographic locations

Exploring green criminology: toward a green criminological revolution

Copper toxicity response influences mesotrophic Synechococcus community structure

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