Toxicity of dextran stabilized fullerene C60 against C6 Glial cells

Biby, T.E.; Prajitha, N.; Ashtami, J.; Sakthikumar, D.; Maekawa, Toru; Mohanan, P.V.

doi:10.1016/j.brainresbull.2019.11.014

Cited by 17 publications

(5 citation statements)

References 51 publications

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“…Such an observation was noted during the safety assessment of fullerene C60 using CHO cells by Biby et al in 2019 [17]. However, on the contrary, ROS induced toxic response induced by similar material was observed in C6 glial cells [18]. Polyhydroxylated fullerene is reported to protect the hippocampus from peroxideinduced toxicity and thereby act as a neuroprotectant [19].…”

Section: Safety and Toxicitymentioning

confidence: 88%

Glial Cell Colony Injured on Exposure to Fullerene Soot Nanoparticles

2020

Lett Appl NanoBioSci

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An expansion in the field of nanotechnology and their applications in medicine and pharmaceuticals have revolutionized the 20th century. Principally it deals with particles exhibiting a size below 100nm at least in a single dimension. Various fields that gain application potentials of nanotechnology include health and medicine, electronics, energy and environment, space research, etc. Advancements in experimental techniques have to lead to the development of multi functionalized nanoparticles like fullerenes, which is one of the allotropic forms of carbon. Fullerene was initially fabricated by laser ablation of graphite in an inert atmosphere. The crude early form consists of a mixture of interlocked carbon cages in its impure version, referred to as fullerene soot. Nature gets exposed to fullerene soot from a cluster of sources, including vehicle, industrial, and other household exhausts. An increasing level of exposure requires toxicity evaluation of such materials in biological systems. Present study addresses in vitro toxicity studies of fullerene soot nanoparticles in C6 glial cells. The study particularly analyzes cell viability assays, morphological evaluations, organelle functionality analysis, live-dead assays, and nuclear integrity measurement. The study confirms the dose-dependent toxicity of the nanomaterials, which further recommends proper precautions to be taken during exposure occasions.

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Section: Safety and Toxicitymentioning

confidence: 88%

Glial Cell Colony Injured on Exposure to Fullerene Soot Nanoparticles

2020

Lett Appl NanoBioSci

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“…Fullerenes also present contradicting results. On one hand, it was found that fullerene nanoparticles induced DNA breakage [177] and oxidative damage to cellular membranes due to ROS generation [178,179]. However, other studies [180] demonstrated no cytotoxic effect on fullerenes.…”

Section: Limitations and Toxicitymentioning

confidence: 99%

Advances in Biodegradable 3D Printed Scaffolds with Carbon-Based Nanomaterials for Bone Regeneration

et al. 2020

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Bone possesses an inherent capacity to fix itself. However, when a defect larger than a critical size appears, external solutions must be applied. Traditionally, an autograft has been the most used solution in these situations. However, it presents some issues such as donor-site morbidity. In this context, porous biodegradable scaffolds have emerged as an interesting solution. They act as external support for cell growth and degrade when the defect is repaired. For an adequate performance, these scaffolds must meet specific requirements: biocompatibility, interconnected porosity, mechanical properties and biodegradability. To obtain the required porosity, many methods have conventionally been used (e.g., electrospinning, freeze-drying and salt-leaching). However, from the development of additive manufacturing methods a promising solution for this application has been proposed since such methods allow the complete customisation and control of scaffold geometry and porosity. Furthermore, carbon-based nanomaterials present the potential to impart osteoconductivity and antimicrobial properties and reinforce the matrix from a mechanical perspective. These properties make them ideal for use as nanomaterials to improve the properties and performance of scaffolds for bone tissue engineering. This work explores the potential research opportunities and challenges of 3D printed biodegradable composite-based scaffolds containing carbon-based nanomaterials for bone tissue engineering applications.

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“…However, non-functionalized fullerenes are insoluble in water and have low biocompatibility. The most common solution to this problem is the synthesis of hydroxyl derivatives of fullerenes, namely fullerenols [29][30][31] and complexes of fullerenes with biocompatible polymers, such as polyvinylpyrrolidone (PVP) [32,33], polyethylene glycol [34,35], γ-cyclodextrin [36,37], dextran [38] and others. At the same time, the synthesis of such water-soluble fullerene derivatives does not solve the problem of their selective accumulation in tumor cells, whereas the creation of conjugates, including with FA, allows us to overcome this problem [39].…”

Section: Introductionmentioning

confidence: 99%

Fullerene C60 Conjugate with Folic Acid and Polyvinylpyrrolidone for Targeted Delivery to Tumor Cells

Borisenkova,

Bolshakova,

Titova

et al. 2024

IJMS

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The use of targeted drug delivery systems, including those based on selective absorption by certain receptors on the surface of the target cell, can lead to a decrease in the minimum effective dose and the accompanying toxicity of the drug, as well as an increase in therapeutic efficacy. A fullerene C60 conjugate (FA-PVP-C60) with polyvinylpyrrolidone (PVP) as a biocompatible spacer and folic acid (FA) as a targeting ligand for tumor cells with increased expression of folate receptors (FR) was obtained. Using 13C NMR spectroscopy, FT-IR, UV-Vis spectrometry, fluorometry and thermal analysis, the formation of the conjugate was confirmed and the nature of the binding of its components was established. The average particle sizes of the conjugate in aqueous solutions and cell culture medium were determined using dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA). The FA-PVP-C60 showed antiradical activity against •DPPH, •OH and O2•−, but at the same time, it was shown to generate 1O2. It was found that the conjugate in the studied concentration range (up to 200 μg/mL) is non-toxic in vitro and does not affect the cell cycle. To confirm the ability of the conjugate to selectively accumulate through folate-mediated endocytosis, its uptake into cells was analyzed by flow cytometry and confocal microscopy. It was shown that the conjugate is less absorbed by A549 cells with low FR expression than by HeLa, which has a high level of expression of this receptor.

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Toxicity of dextran stabilized fullerene C60 against C6 Glial cells

Cited by 17 publications

References 51 publications

Glial Cell Colony Injured on Exposure to Fullerene Soot Nanoparticles

Glial Cell Colony Injured on Exposure to Fullerene Soot Nanoparticles

Advances in Biodegradable 3D Printed Scaffolds with Carbon-Based Nanomaterials for Bone Regeneration

Fullerene C60 Conjugate with Folic Acid and Polyvinylpyrrolidone for Targeted Delivery to Tumor Cells

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