Toxicity of fatty acid 18:5n3 fromgymnodinium cf.mikimotoi: II. intracellular pH and K+ uptake in isolated trout hepatocytes

Fossat, B.; Porthé-Nibelle, Jacqueline; Sola, F. Gil de; Masoni, A.; Gentien, Patrick; Bodennec, Guy

doi:10.1002/(sici)1099-1263(199907/08)19:4<275::aid-jat578>3.0.co;2-b

Cited by 27 publications

(11 citation statements)

References 13 publications

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“…Both K. mikimotoi and K. papilionacea were shown to produce potentially allelopathic sterols, and ichtyotoxic polyunsaturated fatty acids (PUFA) which toxicity is enhanced by the presence of reactive oxygen species (Mooney et al, 2007). The PUFA produced by K. mikimotoi were toxic to a variety of tissue cultures, reduced bacterial bioluminescence, were allelopathic to diatoms, cryptophytes, raphidophytes, and prasinophytes, in all cases disrupting cell membranes (Bodennec et al, 1995;Fossat et al, 1999;Sola et al, 1999;Arzul et al, 2000;Gentien et al, 2007;Chang, 2011). High levels of superoxides with allelopathic activity to other microalgae and bacteria were also found to be produced by K. mikimotoi (Yamasaki et al, 2004;Marshall et al, 2005a,b).…”

Section: Harmful Algal Species Treatmentsmentioning

confidence: 99%

Differential inimical effects of Alexandrium spp. and Karenia spp. on cleavage, hatching, and two larval stages of Japanese pearl oyster Pinctada fucata martensii

Basti

Nagai

Go³

et al. 2015

Harmful Algae

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Section: Harmful Algal Species Treatmentsmentioning

confidence: 99%

Differential inimical effects of Alexandrium spp. and Karenia spp. on cleavage, hatching, and two larval stages of Japanese pearl oyster Pinctada fucata martensii

Basti

Nagai

Go³

et al. 2015

Harmful Algae

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“…Arzul et al (1995) found that polyunsaturated fatty acids (PUFAs) from K. mikimotoi inhibit both diatom growth and bacterial bioluminescence and are also hemolytic. Multiple investigators have observed that K. mikimotoi produces lipophylic toxins and observed toxic effects from a variety of PUFAs in tissue culture studies Fossat et al, 1999;Sola et al, 1999). Gentien et al (2007) also observed allelopathic effects of K. mikimotoi PUFAs on diatoms and that these compounds also show a degree of autotoxicity.…”

Section: Allelochemistry and Toxin Productionmentioning

confidence: 99%

Karenia: The biology and ecology of a toxic genus

2012

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“…Karenia sp. cells are known to produce allelopathic and ichtyotoxic compounds (Gentien and Arzul 1990) but also haemolysins, which in vitro induce red blood cells lysis (Arzul et al 1995;Fossat et al 1999;Sola et al 1999;Jenkinson and Arzul 2000) and fast-degrading toxins responsible for autotoxicity in K. mikimotoi cultures (Gentien et al 2007). Cells of K. selliformis also produce a wellcharacterised toxin referred to as gymnodimine (Seki et al 1995(Seki et al , 1996Mackenzie et al 1996), which caused death of oyster larvae exposed to whole cultures, culture filtrates or sonicated cell extracts of Karenia sp.…”

Section: Effect Of Harmful Algal Cells On Bivalve Haemocytes and Theimentioning

confidence: 99%

In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs

et al. 2011

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Harmful algal blooms (HABs) can have both lethal and sublethal impacts on shellfish. To understand the possible roles of haemocytes in bivalve immune responses to HABs and how the algae are affected by these cells (haemocytes), in vitro tests between cultured harmful algal species and haemocytes of the northern quahog (= hard clam) Mercenaria mercenaria, the soft-shell clam Mya arenaria, the eastern and Pacific oysters Crassostrea virginica and Crassostrea gigas and the Manila clam Ruditapes philippinarum were carried out. Within their respective ranges of distribution, these shellfish species can experience blooms of several HAB species, including Prorocentrum minimum, Heterosigma akashiwo, Alexandrium fundyense, Alexandrium minutum and Karenia spp.; thus, these algal species were chosen for testing. Possible differences in haemocyte variables attributable to harmful algae and also effects of haemolymph and haemocytes on the algae themselves were measured. Using microscopic and flow cytometric observations, changes were measured in haemocytes, including cell morphology, mortality, phagocytosis, adhesion and reactive oxygen species (ROS) production, as well as changes in the physiology and the characteristics of the algal cells, including mortality, size, internal complexity and chlorophyll fluorescence. These experiments suggest different effects of the several species of harmful algae upon bivalve haemocytes. Some harmful algae act as immunostimulants, whereas others are immunosuppressive. P. minimum appears to activate haemocytes, but the other harmful algal species tested seem to cause a suppression of immune functions, generally consisting of decreases in phagocytosis, production of ROS and cell adhesion and besides cause an increase in the percentage of dead haemocytes, which could be attributable to the action of chemical toxins. Microalgal cells exposed to shellfish haemolymph generally showed evidence of algal degradation, e.g. loss of chlorophyll fluorescence and modification of cell shape. Thus, in vitro tests allow a better understanding of the role of the haemocytes and the haemolymph in the defence mechanisms protecting molluscan shellfish from harmful algal cells and could also be further developed to estimate the effects of HABs on bivalve molluscs in vivo.

show abstract

Toxicity of fatty acid 18:5n3 fromgymnodinium cf.mikimotoi: II. intracellular pH and K+ uptake in isolated trout hepatocytes

Cited by 27 publications

References 13 publications

Differential inimical effects of Alexandrium spp. and Karenia spp. on cleavage, hatching, and two larval stages of Japanese pearl oyster Pinctada fucata martensii

Differential inimical effects of Alexandrium spp. and Karenia spp. on cleavage, hatching, and two larval stages of Japanese pearl oyster Pinctada fucata martensii

Karenia: The biology and ecology of a toxic genus

In vitro interactions between several species of harmful algae and haemocytes of bivalve molluscs

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