Toxicity of Melastoma dodecandrum Lour. and its effects on lipopolysaccharide‑induced inflammation and oxidative stress

Abstract

Melastoma dodecandrum Lour. (MDL) is component used in traditional Chinese medicine that is widely distributed throughout southern China. MDL has been long utilized in clinical treatment for various conditions, such as inflammation. However, the toxicity and underlying anti-inflammatory mechanism of MDL remain to be elucidated. In the present study, Sprague-Dawley rats received intragastric administration of MDL for 2 months, and the toxicity of MDL was investigated. The rats were treated with lipopolysaccharide (LPS) for 8 h to determine the potential anti-inflammatory mechanism of MDL. The results demonstrated that MDL alone did not affect the expression levels of factors associated with inflammation (IL-1β, IL-6 and TNF-α) and oxidative stress [malondialdehyde (MDA), superoxide dismutase (SOD) and nitric oxide (NO)] in the rat serum and exerted no effects on rat liver and kidneys. By contrast, MDL attenuated LPS-induced inflammation and oxidative stress by regulating specific cytokines, such as IL-1β, IL-6, TNF-α, MDA, SOD and NO in the rat serum and alleviated LPS-induced liver and kidney damage. Additionally, compared with the LPS group, MDL inhibited CD4 + T cell differentiation into Th1 and Th17 cells and enhanced CD4 + T cell differentiation into Th2 and Treg cells. MDL also suppressed reactive oxygen species (ROS) production and mitochondrial apoptosis by modulating mitochondrial apoptosis-related proteins in spleen CD4 + T cells. In conclusion, the results of the present study demonstrated the non-toxic nature of MDL and revealed that it alleviated LPS-induced inflammation and oxidative stress by regulating differentiation and ROS production in CD4 + T cells.

Mitochondrial genome of Machilus pauhoiassembly highlighted the structure variation through long repetitives in ANA species

“…2). In some studies, it has been pointed out that the number of dispersed repeats seems to correlate closely with mitogenome size [31]. However, we still implied a xed structure, jointing two circles together by a long repeat region.…”

Chemical composition, quality attributes and antioxidant activity of stirred-type yogurt enriched with Melastoma dodecandrum Lour fruit powder

“…12,15 Cheng et al 16 identified around thirteen compounds, including twelve flavonoids and one tannin, and around eighteen compounds were identified by Guo-Xun Yang et al 17 Another study characterized approximately 109 compounds contained in MDL fruit, including 26 fatty acids, 26 organic acids, 33 flavonoids, 6 tannins, 10 triterpenoids, 2 steroids, and 6 other compounds. 13 Besides the non-toxic effect of MDL fruit consumption, as shown by Huang et al , 18 recent studies have shown that MDL extract possesses strong antioxidant activity and substantial antidiabetic activity, especially in the regulation of lipid disorder. 19,20 Therefore, fortifying yogurt with MDL fruit powder (MDLP) can improve the chemical composition, quality attributes, antioxidant activity, and physical characteristics of yogurt.…”

Transcriptomic and metabolomic characterization of antibacterial activity ofMelastoma dodecandrum

“…More recently, studies have been carried out to scientifically evaluate M. malabathricum and M. candidum D. Don for acute toxicity, and antibacterial, antioxidant, and immunomodulatory activities (Mamat et al, 2013;Alnajar et al, 2012;Wong et al, 2012;Che Omar et al, 2013;Zheng et al, 2021). Similarly, efforts have been made to identify the chemical constituents of M. dodecandrum and its associated activities (Huang et al, 2021;Xu et al, 2023;Tong et al, 2019;Wang et al, 2017;Yang et al, 2014). However, limited studies have been carried out on evaluating the antimicrobial activity of the plant and identifying associated active compounds or mechanisms, with only a recent paper reporting moderate antibacterial activity of its ethanolic extract against diarrheagenic bacterial pathogens following an activity screen of 32 plants (Kudera et al, 2021).…”