Toxicity of mycotoxins of <i>Fusarium sambucinum</i> for feeding in <i>Galleria mellonella</i>

Mulé, Giuseppina; Dambrosio, Angela; Logrieco, A.; Bottalico, A.

doi:10.1111/j.1570-7458.1992.tb00636.x

Cited by 25 publications

(15 citation statements)

References 17 publications

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“…Several secondary metabolites from Fusarium spp. have been shown to have insecticidal activity and therefore may play a role in G. mellonella larvae killing (Claydon et al 1977, 1979; Mule et al 1992; Strongman et al 1988). In addition, the morphology of the conidia was important for virulence in this pathosystem (Fig 3), however the precise mechanism conferring the higher virulence of macroconidia is unknown.…”

Section: Discussionmentioning

confidence: 99%

Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host

Coleman¹,

Muhammed²,

Kasperkovitz³

et al. 2011

Fungal Biology

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Members of the fungal genus Fusarium are capable of manifesting in a multitude of clinical infections, most commonly in immunocompromised patients. In order to better understand the interaction between the fungus and host, we have developed the larvae of the greater wax moth, Galleria mellonella, as a heterologous host for fusaria. When conidia are injected into the hemocoel of this Lepidopteran system, both clinical and environmental isolates of the fungus are able to kill the larvae at 37°C, although killing occurs more rapidly when incubated at 30°C. This killing was dependent on several other factors besides temperature, including the Fusarium strain, the number of conidia injected, and the conidia morphology, where macroconidia are more virulent than their microconidia counterpart. There was a correlation in the killing rate of Fusarium spp. when evaluated in G. mellonella and a murine model. In vivo studies indicated G. mellonella hemocytes were capable of initially phagocytosing both conidial morphologies. The G. mellonella system was also used to evaluate antifungal agents, and amphotericin B was able to confer a significant increase in survival to Fusarium infected-larvae. The G. mellonella-Fusarium pathogenicity system revealed that virulence of Fusarium spp. is similar, regardless of the origin of the isolate, and that mammalian endothermy is a major deterrent for Fusarium infection and therefore provides a suitable alternative to mammalian models to investigate the interaction between the host and this increasingly important fungal pathogen.

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Section: Discussionmentioning

confidence: 99%

Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host

Coleman¹,

Muhammed²,

Kasperkovitz³

et al. 2011

Fungal Biology

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“…For instance, compounds that restrain aphids from feeding may reduce direct insect damage to crops and indirect damage due to infection with insect-transmitted viruses (Pickett et al 1987). Data on such effects of fungal metabolites towards insect pests are limited (Wright et al 1982;Mulè et al 1992;Amiri et al 1999;Ekesi et al 2001;Ganassi et al 2007). Ganassi et al (2007) showed that some fungal isolates of the genus Trichoderma influenced the feeding preference of Schizaphis graminum, one of the most important pests of cereal crops, suggesting a potential antifeedant activity of these fungi towards aphids.…”

Section: Introductionmentioning

confidence: 97%

Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

et al. 2009

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We report the effects of some bisorbicillinoids isolated from biomass of the fungus Trichoderma citrinoviride on settling and feeding preference of the aphid Schizaphis graminum. Purification of the fungal metabolites was carried out by a combination of column chromatography and thin-layer chromatography using direct and reverse phases. Chemical identification was performed by spectroscopic methods including nuclear magnetic resonance and mass spectrometry. The identified bisorbicillinoids appeared to be bislongiquinolide, its 16,17-dihydro derivative, trichodimerol, and dihydrotrichodimerol. A feeding preference test with alate morphs of S. graminum was used to identify the active fractions. Among the four bisorbicillinoids, dihydrotrichodimerol and bislongiquinolide influenced aphid feeding preference, restraining specimens from settling on leaves treated with metabolites. Taste neurons sensitive to these compounds, particularly to bislongiquinolide, were located on tarsi of the S. graminum alate morphs.

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“…These mycotoxins are non-ribosomal peptides and can be classified into chain peptides or cyclic peptides based on their chemical structure. A number of toxins have been reported from different entomopathogenic fungi, some of them include destruxins (Pedras et al 2002;Liu and Tzeng 2012), bassianolides (Suzuki et al 1977), beauvericins (Wang and Xu 2012), enniatins (Mule et al 1992;Hiraga et al 2005), efrapeptins (Lardy et al 1975) and so on. Chapters 8-11 of this volume provide more details on these mycotoxins.…”

Section: Entomopathogenic Fungimentioning

confidence: 99%

An Introduction to Entomopathogenic Microorganisms

Sree

Varma

2015

Soil Biology

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Toxicity of mycotoxins of Fusarium sambucinum for feeding in Galleria mellonella

Cited by 25 publications

References 17 publications

Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host

Fusarium pathogenesis investigated using Galleria mellonella as a heterologous host

Bisorbicillinoids Produced by the Fungus Trichoderma citrinoviride Affect Feeding Preference of the Aphid Schizaphis graminum

An Introduction to Entomopathogenic Microorganisms

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