Toxicity of polystyrene nanoplastics and zinc oxide to mice

Estrela, Fernanda Neves; Guimarães, Abraão Tiago Batista; Araújo, Amanda Pereira da Costa; Silva, Fabiano Guimarães; Luz, Thiarlen Marinho da; Silva, Abner Marcelino; Pereira, Paulo Sérgio; Malafaia, Guilherme

doi:10.1016/j.chemosphere.2020.129476

Cited by 76 publications

(18 citation statements)

References 96 publications

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“…1 ). Previous research has shown the toxic effects of microplastics on cells and tissues, with effects such as DNA damage and mitochondrial dysfunction, and these underlying mechanisms, as well as activation of apoptotic pathways, may have contributed to the decreased cell viability observed here (Della Torre et al 2014 ; Estrela et al 2020 ; Li et al 2020d ; Wei et al 2021 ).…”

Section: Discussionmentioning

confidence: 66%

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

2021

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Microplastics are ubiquitous environmental pollutants that are a growing concern to many ecosystems, as well as human health. Many of the effects of microplastics on mammalian cells and tissues remain unknown. To address this, we treated L929 murine fibroblasts and Madin–Darby canine kidney (MDCK) epithelial cell lines with 1 μg/mL, 10 μg/mL, or 20 μg/mL of polyethylene (PE) or polystyrene (PS) microspheres in vitro for 6 and 24 h and measured the resulting changes in cell viability, metabolism, and transcriptional expression of inflammatory cytokines and antioxidant enzymes. We observed dose-dependent decreases in cell viability corresponding to increases in doses of both PE and PS. We conducted cell metabolism assays and observed dose-dependent increases in metabolism per cell with increasing doses of both PE and PS. Similarly, we also observed increased expression of the superoxide dismutase-3 gene ( SOD3 ), indicating oxidative stress caused by the microplastics treatments. We also observed increased expression of TNFα , but decreased expression of IFNβ , suggesting different mechanisms by which the microplastics regulate inflammatory responses in mammalian cells. Our results contribute new data to the growing understanding of the effects of microplastics on mammalian cells and indicate complex cellular stress responses to microplastics in the environment.

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Section: Discussionmentioning

confidence: 66%

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

2021

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“…When placed into a brightly lit open field for the first time, rats and mice tend to remain in the periphery of the apparatus or against the walls (thigmotaxis) ( Gould et al, 2009 ). In the present study, we adopted the procedures described in Estrela et al (2021) ), with minor modifications. Briefly, the test consisted of introducing the animals individually into a rectangular arena (16.5 cm height x 31 cm width x 40.5 cm length), of white opaque walls, which were filmed for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Shedding light on the toxicity of SARS-CoV-2-derived peptide in non-target COVID-19 organisms: A study involving inbred and outbred mice

et al. 2022

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“…NPs can cause more potent threats than MPs to mammals because they are small enough to accumulate in different tissues through systemic circulation (Estrela et al 2021 ). In birds, one can predict that NPs might cause behavioral, physiological, and neuroendocrinological changes, although there has been no identified evidence, and further investigations are necessary.…”

Section: Future Directionsmentioning

confidence: 99%

“…Furthermore, NPs have induced adverse effects on the reproductive functions of laboratory mammals (Amereh et al 2020 ; An et al 2021 ; Jin et al 2021 ), such as alterations in sperm morphology and viability, and lower serum testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) in mice and rats (Amereh et al 2020 ). Polystyrene NPs can cause depression and behavioral and cognitive disorders in mice (da Costa Araújo and Malafaia 2021 ; Estrela et al 2021 ). Despite the limited information on the toxicological effects of NPs on non-laboratory model animals, the above-mentioned effects of widely distributed NPs can be inferred to occur in free-living animals.…”

Section: Introductionmentioning

confidence: 99%

Birds and plastic pollution: recent advances

et al. 2021

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Plastic waste and debris have caused substantial environmental pollution globally in the past decades, and they have been accumulated in hundreds of terrestrial and aquatic avian species. Birds are susceptible and vulnerable to external environments; therefore, they could be used to estimate the negative effects of environmental pollution. In this review, we summarize the effects of macroplastics, microplastics, and plastic-derived additives and plastic-absorbed chemicals on birds. First, macroplastics and microplastics accumulate in different tissues of various aquatic and terrestrial birds, suggesting that birds could suffer from the macroplastics and microplastics-associated contaminants in the aquatic and terrestrial environments. Second, the detrimental effects of macroplastics and microplastics, and their derived additives and absorbed chemicals on the individual survival, growth and development, reproductive output, and physiology, are summarized in different birds, as well as the known toxicological mechanisms of plastics in laboratory model mammals. Finally, we identify that human commensal birds, long-life-span birds, and model bird species could be utilized to different research objectives to evaluate plastic pollution burden and toxicological effects of chronic plastic exposure.

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Toxicity of polystyrene nanoplastics and zinc oxide to mice

Cited by 76 publications

References 96 publications

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Polystyrene and Polyethylene Microplastics Decrease Cell Viability and Dysregulate Inflammatory and Oxidative Stress Markers of MDCK and L929 Cells In Vitro

Shedding light on the toxicity of SARS-CoV-2-derived peptide in non-target COVID-19 organisms: A study involving inbred and outbred mice

Birds and plastic pollution: recent advances

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