Toxicity of silver nanoparticles on different tissues of Balb/C mice

Moradi-Sardareh, Hemen; Basir, Hamid Reza Ghasemi; Hassan, Zuhair Mohammad; Davoudi, Maryam; Amidi, Fardin; Paknejad, Maliheh

doi:10.1016/j.lfs.2018.09.001

Cited by 56 publications

(32 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…H&E stained sections showed many collapsed alveoli with thick inter alveolar septa, other alveoli showed ballooned distension. Previous authors mentioned similar results in silver nanoparticles treated lung [43,44] .…”

Section: Discussionsupporting

confidence: 70%

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

Gawish

Shaban

Aal

et al. 2019

Journal of Medical Histology

View full text Add to dashboard Cite

Background: Silver nanoparticles (Ag-Nps) and Titanium dioxide nanoparticles (TiO2-Nps) are well-known nanoproducts. Both of them have many industrial applications. Lung is one of the major target organs for prolonged nanoparticles exposure. Objective: This study was designed to investigate and compare the possible histopathological toxic effect of Ag-Nps & TiO2-Nps on lung and which of them is safer for future using. Materials & methods: 54 adult male albino rats were divided into 3 equal groups; GroupI (control group), Group II that subdivided into two subgroups; Subgroup IIa: Ag-Nps group with (100 mg/kg/day) daily for 4 successive weeks and Subgroup IIb recovery group left for 4 weeks without injection, Group III that subdivided into two subgroups; Subgroup IIIa: TiO2-Nps group with (150 mg/kg/day) for 4 successive weeks and Subgroup IIIb recovery group left for 4 weeks without injection. Lung specimens were processed for light and electron microscope and immunohistochemical expression of caspase-3 and CD-68. Morphometric and statistical analysis were performed. Results: Group IIa showed collapsed alveoli, others showed ballooned distension and marked thickening of inter-alveolar septa. Extensive cellular infiltration was detected. Group IIIa showed focal areas of collapsed alveoli and thick inter-alveolar septa. Mild cellular infiltrations were observed. Areas of extravasation were detected in the interstitium. Group IIIb showed signs of improvement which is more than group IIb. Conclusion: exposure to Ag-Nps showed marked alterations on histological structure of lung, which is more than the alteration caused by TiO2-Nps; in addition, recovery period was proved to ameliorate these changes better in TiO2-Nps.

show abstract

Section: Discussionsupporting

confidence: 70%

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

Gawish

Shaban

Aal

et al. 2019

Journal of Medical Histology

View full text Add to dashboard Cite

show abstract

“…As a result, various studies have been designed to assess the effect of even low doses of silver nanoparticles (AgNPs) on bacterial cell membranes [ 30 , 31 , 32 ]. Results on in vivo toxicity of AgNPs have been reported for various living organisms, such as zebrafish [ 33 , 34 ], catfish [ 35 ], common carp [ 36 ], Corbicula fluminea clam [ 37 ], Caenorhabditis elegans worm [ 38 ], and mice [ 39 , 40 ]. The interaction of metal nanoparticles with nucleic acids is also a hot topic in the field of bioinorganic research, due to the interest in elucidating possible effects of Ag nanoparticles on the synthesis, replication, and structural integrity of DNA and RNA [ 41 ] and the mechanism of DNA–nanoparticle binding [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-Stabilized Ag Nanoparticles with Superior Biocompatibility and Their Synergistic Antibacterial Effect in Mixtures with Essential Oils

et al. 2018

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) are considered a promising alternative to the use of antibiotics in fighting multidrug-resistant pathogens. However, their use in medical application is hindered by the public concern regarding the toxicity of metallic nanoparticles. In this study, rationally designed AgNP were produced, in order to balance the antibacterial activity and toxicity. A facile, environmentally friendly synthesis was used for the electrochemical fabrication of AgNPs. Chitosan was employed as the capping agent, both for the stabilization and to improve the biocompatibility. Size, morphology, composition, capping layer, and stability of the synthesized nanoparticles were characterized. The in vitro biocompatibility and antimicrobial activities of AgNPs against common Gram-negative and Gram-positive bacteria were evaluated. The results revealed that chitosan-stabilized AgNPs were nontoxic to normal fibroblasts, even at high concentrations, compared to bare nanoparticles, while significant antibacterial activity was recorded. The silver colloidal dispersion was further mixed with essential oils (EO) to increase the biological activity. Synergistic effects at some AgNP–EO ratios were observed, as demonstrated by the fractionary inhibitory concentration values. Our results reveal that the synergistic action of both polymer-stabilized AgNPs and essential oils could provide a significant efficiency against a large variety of microorganisms, with minimal side effects.

show abstract

“…According to previous studies, for improvement of the dispersion stability, a desired amount of AgNPs was dispersed in FBS and the protein corona was formed on their surface using sonication. 15,22 The recent study also showed that FBS could be considered as a putative protein corona in the stabilizing of AgNPs. We observed that the purchased nanoparticles agglomerated before dispersing into FBS, which was visible under light microscopy.…”

Section: Discussionmentioning

confidence: 99%

“…To avoid their in-vitro agglomeration, the protein corona was formed on the surface of AgNPs as described previously. 15 Briefly, the desired amount of AgNPs was dispersed in FBS (1 ml) and sonicated for 10 min on ice to obtain a stock solution of AgNPs in FBS (10,000 μg/ml). The stock solution was stored at −20 C. Immediately before each test, the desired suspensions of AgNPs were prepared by diluting the stock solution in DMEM and then sonicated again.…”

Section: Agnps Preparationmentioning

confidence: 99%

The possible effect of silver nanoparticles on glyceraldehyde‐3‐phosphate dehydrogenase activity and formation of amyloid‐like aggregates in MCF‐7 cell line

et al. 2020

Self Cite

View full text Add to dashboard Cite

Silver nanoparticles (AgNPs) are widely used in medicine, however, the underlying mechanisms of their action on cellular signaling have not been completely determined, and fundamental studies are required to clarify them. We aimed to investigate AgNPs effects on glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) as both the internal control gene and the redox‐sensitive enzyme involved in apoptosis‐related pathways and the formation of amyloid aggregates. To achieve this purpose, MCF‐7 cells were treated with different concentrations (0, 3, 22, and 200 μg/ml) of AgNPs and then cell viability, generation of reactive oxygen species (ROS), induction of apoptosis, expression of GAPDH gene, the formation of amyloid aggregates, and GAPDH activity were assessed. The results indicated that treatment with AgNPs significantly reduced cell viability and increased apoptosis in a dose‐dependent manner. The ROS levels increased at lower concentrations of AgNPs (up to 22 μg/ml) and during short‐term exposure (30 min). The level of GAPDH gene expression was significantly upregulated by 1.22, 1.47, and 1.56 fold, in the concentrations of 3, 22, and 200 μg/ml, respectively. The amount of amyloid aggregates was significantly increased in a dose‐dependent manner. The results of enzyme activity showed that AgNPs were affected on the activity of GAPDH protein, however, it has fluctuated that could not be interpreted by our limited data. In conclusion, our results suggested that AgNPs could affect the GAPDH gene expression and enzyme activity, therefore the selection of GAPDH as a gene and protein internal control in the (AgNPs)‐related studies requires careful consideration. Additionally, AgNPs may cause apoptosis due to the increase in the production of amyloid aggregates.

show abstract

Toxicity of silver nanoparticles on different tissues of Balb/C mice

Cited by 56 publications

References 50 publications

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

CEffect of Silver Nanoparticles Versus Titanium Dioxide Nanoparticles on the Lung of Adult Male Albino Rats: A histological and immunohistochemical study

Chitosan-Stabilized Ag Nanoparticles with Superior Biocompatibility and Their Synergistic Antibacterial Effect in Mixtures with Essential Oils

The possible effect of silver nanoparticles on glyceraldehyde‐3‐phosphate dehydrogenase activity and formation of amyloid‐like aggregates in MCF‐7 cell line

Contact Info

Product

Resources

About