Toxicity of Uranium to Microbial Communities in Anaerobic Biofilms

Tapia-Rodríguez, Aída; Luna-Velasco, Antonia; Field, James A.; Sierra-Álvarez, Reyes

doi:10.1007/s11270-012-1154-0

Cited by 18 publications

(15 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 97%

“…For example in Tapia‐Rodríguez et al . () methanogenic and denitrifying cultures under acetate amendment were sensitive to uranium dose (Tapia‐Rodríguez et al ., ), however, contrasting results have been demonstrated in some denitrifying microbial communities in the field (Green et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…In laboratory studies investigating how uranium affects microbes, microbial isolates and enrichment cultures have been exposed to controlled amounts of uranium (Payne et al, 2002;Martinez et al, 2006;Vanengelen et al, 2011;Choudhary et al, 2012;Kumar et al, 2012;Nongkhlaw et al, 2012;Tapia-Rodr ıguez et al, 2012). Removing some of the assemblage complexity allowed Vanengelen et al (2011) to demonstrate that one mechanism of uranium toxicity is the preferential binding of the pyrroloquinoline quinone (PQQ) cofactor to uranyl ions (UO 21 2 ) instead of calcium ions (Ca 21 ).…”

Section: Introductionmentioning

confidence: 99%

“…This cofactor is associated with catabolic enzymes in a range of bacterial phyla (Vanengelen et al, 2011). Additionally, inhibition concentrations have been derived for numerous isolates (Payne et al, 2002;Kumar et al, 2012;Nongkhlaw et al, 2012) and enrichment cultures (Tapia-Rodr ıguez et al, 2012), finding uranium-tolerances vary significantly. These suggest that uranium may sometimes impact certain microbes and microbial functions disproportionately.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of uranium concentration on microbial community structure and functional potential

Sutcliffe

Chariton

Harford³

et al. 2017

Environmental Microbiology

View full text Add to dashboard Cite

Located in the Northern Territory of Australia, Ranger uranium mine is directly adjacent to the UNESCO World Heritage listed Kakadu National Park, with rehabilitation targets needed to ensure the site can be incorporated into the park following the mine's closure in 2026. This study aimed to understand the impact of uranium concentration on microbial communities, in order to identify and describe potential breakpoints in microbial ecosystem services. This is the first study to report in situ deployment of uranium-spiked sediments along a concentration gradient (0-4000 mg U kg ), with the study design maximising the advantages of both field surveys and laboratory manipulative studies. Changes to microbial communities were characterised through the use of amplicon and shotgun metagenomic next-generation sequencing. Significant changes to taxonomic and functional community assembly occurred at a concentration of 1500 mg U kg sediment and above. At uranium concentrations of ≥ 1500 mg U kg , genes associated with methanogenic consortia and processes increased in relative abundance, while numerous significant changes were also seen in the relative abundances of genes involved in nitrogen cycling. Such alterations in carbon and nitrogen cycling pathways suggest that taxonomic and functional changes to microbial communities may result in changes in ecosystem processes and resilience.

show abstract

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of uranium concentration on microbial community structure and functional potential

Sutcliffe

Chariton

Harford³

et al. 2017

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…The initial concentrations of the compounds that caused a 20%, 50% and 80% decrease in the methanogenic activity compared to the uninhibited control (IC 20 , IC 50 and IC 80 ) were estimated as described elsewhere (Tapia-Rodriguez et al, 2012). …”

Section: Methodsmentioning

confidence: 99%

Microbial toxicity of ionic species leached from the II-VI semiconductor materials, cadmium telluride (CdTe) and cadmium selenide (CdSe)

et al. 2016

Self Cite

View full text Add to dashboard Cite

This work investigated the microbial toxicity of soluble species that can potentially be leached from the II–VI semiconductor materials, cadmium telluride and cadmium selenide. The soluble ions tested included: cadmium, selenite, selenate, tellurite, and tellurate. Their toxicity towards the acetoclastic and hydrogen-consuming trophic groups in a methanogenic consortium as well as towards a bioluminescent marine bacterium, Aliivibrio fischeri (Microtox® test), was assessed. The acetoclastic methanogenic activity was the most affected as evidenced by the low 50% inhibiting concentrations (IC50) values obtained of 8.6 mg L−1 for both cadmium and tellurite, 10.2 mg L−1 for tellurate, and 24.1 mg L−1 for selenite. Both tellurium oxyanions caused a strong inhibition of acetoclastic methanogenesis at low concentrations, each additional increment in concentration provided progressively less inhibition increase. In the case of the hydrogenotrophic methanogenesis, cadmium followed by selenite caused the greatest inhibition with IC50 values of 2.9 and 18.0 mg L−1, respectively. Tellurite caused a moderate effect as evidenced by a 36.8% inhibition of the methanogenic activity at the highest concentration tested, and a very mild effect of tellurate was observed. Microtox® analyses showed a noteworthy inhibition of cadmium, selenite, and tellurite with 50% loss in bioluminescence after 30 min of exposure of 5.5, 171.1, and 458.6 mg L−1, respectively. These results suggest that the leaching of cadmium, tellurium and selenium ions from semiconductor materials can potentially cause microbial toxicity.

show abstract