Toxicity of Vipera palaestinae venom and antagonistic effects of methanolic leaf extract of Eryngium creticum lam

Nusair, Shreen Deeb; Ahmad, Mohammad I.

doi:10.1016/j.toxicon.2019.05.006

Cited by 13 publications

(7 citation statements)

References 37 publications

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“…Protein was measured by a previously modified Bradford (1976) method to fit microassay protein quantification; bovine serum albumin was the standard [12] , [13] . The protein standard was diluted 1:1, 1:10, 1:100, and 1:1000 using 0.1 M PBS (pH 7.4) to construct the standard curve.…”

Section: Methodsmentioning

confidence: 99%

Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose

et al. 2022

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“…The root is used traditionally for the treatment of kidney stones, liver diseases, skin diseases, infections, edema, sinusitis, inflammations, poisoning, anemia, and infertility. 1 Regarding the reported biological activities, Eryngium criticum is reported to possess antisnake and antiscorpion venom properties 2 and antimicrobial, 3 antimalarial, antileishmanial, 4 cytotoxic, 5 and hypoglycemic activities. 6 There are some studies addressing the chemical constituents of essential oils.…”

Section: Introductionmentioning

confidence: 99%

“…Regarding the reported biological activities, Eryngium criticum is reported to possess antisnake and antiscorpion venom properties and antimicrobial, antimalarial, antileishmanial, cytotoxic, and hypoglycemic activities …”

Section: Introductionmentioning

confidence: 99%

Eryngium creticum L.: Chemical Characterization, SARS-CoV-2 Inhibitory Activity, and In Silico Study

2022

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Phytochemical investigation of Eryngium creticum L. has resulted in isolation of five compounds, including four compounds that are reported from the plant for the first time. Compound 1 was identified as ( E )-rosmarinic acid, meanwhile, compound 2 was isolated as an ( E / Z )-rosmarinic acid mixture. Interestingly, the E / Z -isomeric mixture was about 4 times as active as the single E -isomer toward the severe acute respiratory syndrome coronavirus 2 3-chymotrypsin-like protease (3CL pro ), IC 50 = 6.062 and 25.75 μM, respectively. Utilizing combined molecular docking and molecular dynamics (MD) techniques, the binding affinities and features of the isolated compounds were evaluated against 3CL pro . Compound 2 Z demonstrated a higher binding affinity for 3CL pro than 2 E , with docking scores of −8.9 and −8.5 kcal/mol and MM-GBSA/150 ns MD binding energies of −26.5 and −22.1 kcal/mol, respectively. This justifies the superior activity of the E / Z -isomeric mixture versus the single E -isomer. Structural and energetic analyses revealed the stability of 2 Z and 2 E compared to the reference HIV-1 protease inhibitor, lopinavir. Besides, DFT calculations demonstrated the more energetic stability of 2 E compared to 2 Z , which justifies the difficulty in isolating the Z -isomer in a pure form, where it readily isomerizes to the E -isomer.

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“…Antivenom is currently the most effective and commonly used treatment for Bungarus multicinctus bites. According to the WHO, because it is expensive and difficult to store, antivenom is challenging to obtain in economically disadvantaged and remote rural areas, and there is still great risk associated with antivenom treatment [ 8 ]. In China, horse-derived antivenom is currently used in clinical, but the allergic reaction rate can be high, ranging from 3% to 88% [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacological Study on the Mechanism of Cynanchum paniculatum (Xuchangqing) in the Treatment of Bungarus multicinctus Bites

Zeng

Hou

et al. 2022

BioMed Research International

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Background. Bungarus multicinctus is one of the top ten venomous snakes in China. Its venom is mainly neurotoxin-based. Novel antivenom drugs need to be further researched and developed. Objective. This study aimed to explore the molecular mechanism of Cynanchum paniculatum in treating Bungarus multicinctus bites based on network pharmacology. Material and methods. The potential active ingredients of Cynanchum paniculatum were screened and their SDF structures were obtained using the PubChem database and imported into the SwissTargetPrediction database, and targets were obtained for the antitoxin effects of Cynanchum paniculatum in the treatment of Bungarus multicinctus bites. The Cynanchum paniculatum-active compound-potential target network and protein-protein interaction network were constructed by using Cytoscape software, and then biological function analysis and KEGG pathway enrichment analysis were performed using the DAVID. Results. Seven potential active components (cynapanoside C, cynatratoside B, tomentolide A, sitosterol, sarcostin, tomentogenin, and paeonol) and 286 drug targets were obtained, including 30 key targets for the treatment of bungarotoxin toxicity. The active components mainly acted on PIK3CA, MAPK1, MAP2K1, JAK2, FYN, ACHE, CHRNA7, CHRNA4, and CHRNB2, and they antagonized the inhibitory effect of bungarotoxin on the nervous system through cholinergic synapses and the neurotrophin signaling pathway. Conclusions. Cynanchum paniculatum exerts a therapeutic effect on Bungarus multicinctus bites through multiple active components, multiple targets, and multiple pathways. The findings provide a theoretical basis for the extraction of active components of Cynanchum paniculatum and for related antivenom experiments.

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Toxicity of Vipera palaestinae venom and antagonistic effects of methanolic leaf extract of Eryngium creticum lam

Cited by 13 publications

References 37 publications

Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose

Postmortem sampling time effect on toxicity biomarkers in rats exposed to an acute lethal methomyl dose

Eryngium creticum L.: Chemical Characterization, SARS-CoV-2 Inhibitory Activity, and In Silico Study

Network Pharmacological Study on the Mechanism of Cynanchum paniculatum (Xuchangqing) in the Treatment of Bungarus multicinctus Bites

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