Toxicity of Zinc Oxide Nanoparticles in Lung Tissue After Repeated Oral Administration

Shokouhian, Ataollah; Saman, Soheili; Moradhaseli, Saeed; Fazli, Leila; Ardestani, Mehdi Shafiee; Ghorbani, Masoud

doi:10.3844/ajptsp.2013.148.154

Cited by 9 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shearing of DNA of splenocytes and thymocytes was noted. Similar data in rat lung after oral administration of ZnO NPs were reported . In the same line, the study of Tian et al revealed elevations of proinflammatory cytokines in the serum and the brain of mice after intraperitoneal injection of ZnO NPs.…”

Section: Discussionsupporting

confidence: 78%

Effect of orally administered zinc oxide nanoparticles on albino rat thymus and spleen

Abass

Selim

et al. 2017

IUBMB Life

View full text Add to dashboard Cite

This study aimed to evaluate the toxicological effects of oral intake of Zinc oxide nanoparticles (ZnO NPs) on the structure of thymus and spleen. Twenty-four young male Wistar albino rats were assigned into two groups: group I (control) and group II (ZnO NPs treated group).The thymus and spleen were analyzed biochemically, histopathologically and immunohistochemically. After ZnO NPs intake, hematologically, the total leucocytic count was significantly increased while the RBCs and platelets counts and Hb % were significantly decreased. Biochemically, a significant decrease in serum total antioxidant capacity and anti-inflammatory cytokines including interleukin 4 and 10 (IL-4 and IL-10) levels was noted. While a significant increase in splenic and thymic malondialdehyde (MDA) and DNA shearing, as well as the studied proinflammatory cytokines; IL-1β, tumor necrotic factor (TNF-α) and interferon (INF-γ) levels was detected. Notably, we noted upregulation of the immunomodulatory [CD3, CD11b, heme oxygenase (HO-1)] and the inflammatory [toll-like receptor 4 and 6 (TLR4 and TLR6)] genes. Histopathologically, degenerative changes were detected in thymus and spleen of ZnO NPs treated group. While the immunohistochemical analysis of the ZnO NPs treated group revealed a decrease in the number of cells expressed positive reactions of anti-PCNA and an increase in the number of cells expressed positive reaction of anti-p53 in the thymus and spleen. In conclusion, ZnO NPs induced obvious immunotoxicity in the thymus and spleen, where oxidative/inflammatory pathway may be the potential mechanism underlying this immunotoxicity. © 2017 IUBMB Life, 69(7):528-539, 2017.

show abstract

Section: Discussionsupporting

confidence: 78%

Effect of orally administered zinc oxide nanoparticles on albino rat thymus and spleen

Abass

Selim

et al. 2017

IUBMB Life

View full text Add to dashboard Cite

show abstract

“…9 ). GSH levels have been reported to be decreased significantly after oral administration of ZnO NPs in lung tissue [55] . In the present study, the ZnO NPs showed an insignificant difference in GSH activity compared to their ionic forms.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of the cytotoxic and genotoxic potentials of zinc oxide and titanium dioxide nanoparticles

2015

View full text Add to dashboard Cite

Nanoparticles (NPs) of zinc oxide (ZnO) and titanium dioxide (TiO2) are receiving increasing attention due to their widespread applications. The aim of this study was to evaluate the toxic effect of ZnO and TiO2 NPs at different concentrations (50, 100, 250 and 500 ppm) and compare them with their respective salts using a battery of cytotoxicity, and genotoxicity parameters. To evaluate cytotoxicity, we have used human erythrocytes and for genotoxic studies human lymphocytes have been used as in vitro model species. Concentration dependent hemolytic activity to RBC's was obtained for both NPs. ZnO and TiO2 NPs resulted in 65.2% and 52.5% hemolysis at 250 ppm respectively indicating that both are cytotoxic to human RBCs. Antioxidant enzymes assays were also carried out in their respective hemolysates. Both nanoparticles were found to generate reactive oxygen species (ROS) concomitant with depletion of glutathione and GST levels and increased SOD, CAT and lipid peroxidation in dose dependent manner. ZnO and TiO2 NPs exerted roughly equal oxidative stress in terms of aforementioned stress markers. Genotoxic potential of both the NPs was investigated by in vitro alkaline comet assay. DNA damage induced by the NPs was concentration dependent and was significantly greater than their ionic forms at 250 and 500 ppm concentrations. Moreover, the nanoparticles of ZnO were significantly more genotoxic than those of TiO2 at higher concentrations. The toxicity of these NPs is due to the generation of ROS thereby causing oxidative stress.

show abstract

“…Group 2 (querectin-treated group) rats received 100 mg/kg body weight querectin orally once daily for 12 weeks dissolved in distilled water, and this oral protective dose of querectin has been reported previously as a good protective daily dose in rats [15]. Group 3 (low ZnO-treated group) rats received 100 mg/kg body weight ZnO per day for 12 weeks orally dissolved in distilled water, meanwhile group 4 rats received high ZnO-treated group (400 mg/kg body weight) once daily for 12 weeks [16,17]. Group 5 rats were treated with querectin and low ZnO dose as in groups and 3, whereas group 6 rats were treated with querectin and high ZnO dose as in groups 2 and 4.…”

Section: Experimental Designmentioning

confidence: 99%

Querectin Alleviates Zinc Oxide Nanoreprotoxicity in Male Albino Rats

Hussein

Ali

Saadeldin

et al. 2016

J Biochem Mol Toxicol

View full text Add to dashboard Cite

Zinc oxide nanopartciles (ZnONPs) involved in advanced technologies, and their wide-scale use in consumer market makes human beings more prone to the exposure to ZnONPs. The present study was undertaken to evaluate amelioration of ZnONP-induced toxicities with querectin in male albino rats. ZnONPs-treated rats showed a significant decrease in sperm cell count, sperm motility, live and normal sperms, as well as serum testosterone level. Severe histopathological damage with a significant increase in lipid peroxidation and a decrease in antioxidant enzymes activity and the GSH level were observed in the affected testis. Relative quantitative polymerase chain reaction results showed a significant decrease in antioxidant enzymes (superoxide dismutase and catalase) and a significant decrease in 3β-HSD, 17β-HSD, and Nr5A1 transcripts. Rats-administered querectin along with ZnONPs showed less toxic effects on all studied reproductive traits and mRNA transcripts. Our results suggest that querectin is beneficial for preventing or ameliorating ZnONP reproductive toxicities in males.

show abstract

Toxicity of Zinc Oxide Nanoparticles in Lung Tissue After Repeated Oral Administration

Cited by 9 publications

References 22 publications

Effect of orally administered zinc oxide nanoparticles on albino rat thymus and spleen

Effect of orally administered zinc oxide nanoparticles on albino rat thymus and spleen

Comparative study of the cytotoxic and genotoxic potentials of zinc oxide and titanium dioxide nanoparticles

Querectin Alleviates Zinc Oxide Nanoreprotoxicity in Male Albino Rats

Contact Info

Product

Resources

About