Toxicity potentials of the nutraceutical Moringa oleifera at supra-supplementation levels

Asare, George Awuku; Gyan, Ben; Bugyei, Kwasi Agyei; Adjei, Samuel; Mahama, Razak; Addo, Phyllis; Otu-Nyarko, Lydia; Wiredu, Edwin K.; Nyarko, Alexander Kwadwo

doi:10.1016/j.jep.2011.11.009

Cited by 145 publications

(102 citation statements)

References 27 publications

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“…Nevertheless, i.p. injection presented 20% and 80% mortality in Wistar albino mice at doses of 1000 and 2000 mg/kg, with LD 50 of 1585 mg/kg and acute administration at 3000mg/kg reduces urea and albumin levels, indicating liver and renal dysfunction [119] probably initiated by toxicants such as isothiocinates and glycosides during biotransformation and corroborating those outcomes described by [115] and [118], whose mice presented biochemical alterations suggestive of renal damage. An opposing discovery to all previous researches divulged, for the first time, showed that M. oleifera has genotoxic potential at higher doses (3000 mg/kg), increasing significantly the number of polychromatic micronucleated erythrocytes derived from bone marrow of rodents (20.2 ± 4.0 cells/1000 cells) when compared to control (0.9% saline) [119].…”

Section: Leaves Flowers and Rootssupporting

confidence: 87%

Safety and Efficacy of Moringa oleifera Lamarck (1785) — Therapeutic and Toxicological Properties

Ferreira¹,

Araújo²,

Freitas³

et al. 2014

Pharmacology and Therapeutics

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Section: Leaves Flowers and Rootssupporting

confidence: 87%

Safety and Efficacy of Moringa oleifera Lamarck (1785) — Therapeutic and Toxicological Properties

Ferreira¹,

Araújo²,

Freitas³

et al. 2014

Pharmacology and Therapeutics

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“…The damage can be reverted by the DNA repair system or may persist, inducing genotoxicity and even mutagenesis. To evaluate the mutagenic and genotoxic effects of numerous compounds, comet and micronucleus tests are well established in the indication of the extent and severity of the interaction between the compounds studied and DNA [47,48]. Furthermore, these tests are validated by international agencies that evaluate the safety of chemical agents.…”

Section: Discussionmentioning

confidence: 99%

Andiroba Oil (Carapa guianensis Aublet) Nanoemulsions: Development and Assessment of Cytotoxicity, Genotoxicity, and Hematotoxicity

Milhomem-Paixão

Fascineli

Muehlmann

et al. 2017

Journal of Nanomaterials

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Andiroba oil (AO) is obtained from an Amazonian plant and is used in traditional medicine. We carried out a comparative study to test the cytotoxicity, genotoxicity, and hematotoxicity of the oil and its nanoemulsion (AN) in vitro (fibroblasts, lineage NIH/3T3) and in vivo (Swiss mice). The AN was characterized by DLS/Zeta, and its stability was investigated for 120 days. The biological activity of AN was assessed in vitro by MTT test and cell morphology analyses and in vivo by micronucleus, comet, and hematotoxicity tests. The AN presented a hydrodynamic diameter (Hd) of 142.5 ± 3.0 and PDI of 0.272 ± 0.007 and good stability at room temperature. The MTT test evidenced the cytotoxicity of AO and of AN only at their highest concentrations, but AN showed lower cytotoxicity than AO. A lower cytotoxicity of AN, when compared to AO, is in fact an interesting data suggesting that during therapeutic application there will be a lower impact in the cell viability of healthy cells. Cytotoxicity, genotoxicity, and hematotoxicity were not observed in vivo. These tests on the biological and toxicological effects of andiroba oil and nanostructured oil are still initial ones but will give a direction to future application in cosmetics and/or the development of new phytotherapics.

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“…As recently reviewed by Stohs and Hartman (2015), a considerable number of human and animal as well as in vitro studies indicate that various preparations of M. oleifera leaves and other plant parts have demonstrated a very high degree of safety. On the other hand, some reports on adverse effects of M. oleifera isolated compounds or preparations have been described in animal or in vitro studies, such as effects in female reproductive system (Sethi et al, 1988;Shukla et al, 1988;Prakash et al, 1987), genoand cytotoxic activities (Villasenor et al, 1989;Rolim et al, 2011;Asare et al, 2012;Araújo et al, 2013), hypotensive effects (Faizi et al, 1994(Faizi et al, , 1998, source of antinutritional factors (Igwilo et al, 2013(Igwilo et al, , 2014, hepatic and renal damage (Oyagbemi et al, 2013), and acidosis (Omabe et al, 2014). In parallel to the scientific data, for hundreds of years several parts of M. oleifera have been consumed by humans and animals in many tropical and subtropical countries with no reports of toxic effects (Fahey, 2005;Thurber and Fahey, 2009).…”

Section: History Of Safe Use Of M Oleifera Speciesmentioning

confidence: 99%

Food safety assessment of an antifungal protein from Moringa oleifera seeds in an agricultural biotechnology perspective

Pinto

Farias

Carvalho

et al. 2015

Food and Chemical Toxicology

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Mo-CBP3 is an antifungal protein produced by Moringa oleifera which has been investigated as potential candidate for developing transgenic crops. Before the use of novel proteins, food safety tests must be conducted. This work represents an early food safety assessment of Mo-CBP3, using the two-tiered approach proposed by ILSI. The history of safe use, mode of action and results for amino acid sequence homology using the full-length and short contiguous amino acids sequences indicate low risk associated to this protein. Mo-CBP3 isoforms presented a reasonable number of alignments (>35% identity) with allergens in a window of 80 amino acids. This protein was resistant to pepsin degradation up to 2 h, but it was susceptible to digestion using pancreatin. Many positive attributes were presented for Mo-CBP3. However, this protein showed high sequence homology with allergens and resistance to pepsin digestion that indicates that further hypothesis-based testing on its potential allergenicity must be done. Additionally, animal toxicity evaluations (e.g. acute and repeated dose oral exposure assays) must be performed to meet the mandatory requirements of several regulatory agencies. Finally, the approach adopted here exemplified the importance of performing an early risk assessment of candidate proteins for use in plant transformation programs.

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Toxicity potentials of the nutraceutical Moringa oleifera at supra-supplementation levels

Cited by 145 publications

References 27 publications

Safety and Efficacy of Moringa oleifera Lamarck (1785) — Therapeutic and Toxicological Properties

Safety and Efficacy of Moringa oleifera Lamarck (1785) — Therapeutic and Toxicological Properties

Andiroba Oil (Carapa guianensis Aublet) Nanoemulsions: Development and Assessment of Cytotoxicity, Genotoxicity, and Hematotoxicity

Food safety assessment of an antifungal protein from Moringa oleifera seeds in an agricultural biotechnology perspective

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