Toxicity Studies and Biomedical Applications of Graphene Oxide

Kovbasyuk, Larisa; Mokhir, Andriy

doi:10.1002/9781119069447.ch11

Cited by 3 publications

(4 citation statements)

References 71 publications

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“…GO is also found responsible for the resulted membrane pore formation and water molecules flowing, which indicates that the shielding of hydrophobic domains of GO by hydrophilic polymers can reduce its toxicity (Figure B) . Another path of cell membrane damage by FGNs is the extraction of phospholipids from the lipid bilayer due to the strong adsorptions between FGNs and lipid molecules. , The direct contact and endocytosis of FGNs will both induce oxidative stress and ROS . To figure out the complex processes of GO-induced oxidative stress in living cells, Mokhir and co-workers studied different redox-active groups modified GOs, such as the Mn 2+ ions, C-centered radicals, and endoperoxides .…”

Section: Fgns–bioorganism Interactionsmentioning

confidence: 99%

“…136,137 The direct contact and endocytosis of FGNs will both induce oxidative stress and ROS. 138 To figure out the complex processes of GO-induced oxidative stress in living cells, Mokhir and co-workers studied different redox-active groups modified GOs, such as the Mn 2+ ions, C-centered radicals, and endoperoxides. 139 Their study reveals for the first time that endoperoxide can generate a significant reactive oxygen species by using human cervical cancer cell line as a model system, which indicates that the surface-bound endoperoxide groups are responsible for the GO-induced oxidative stress and cytotoxicity.…”

Section: Fgns−mammalian Cell Toxicity and Interactionsmentioning

confidence: 99%

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Functional Graphene Nanomaterials Based Architectures: Biointeractions, Fabrications, and Emerging Biological Applications

et al. 2017

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Functional graphene nanomaterials (FGNs) are fast emerging materials with extremely unique physical and chemical properties and physiological ability to interfere and/or interact with bioorganisms; as a result, FGNs present manifold possibilities for diverse biological applications. Beyond their use in drug/gene delivery, phototherapy, and bioimaging, recent studies have revealed that FGNs can significantly promote interfacial biointeractions, in particular, with proteins, mammalian cells/stem cells, and microbials. FGNs can adsorb and concentrate nutrition factors including proteins from physiological media. This accelerates the formation of extracellular matrix, which eventually promotes cell colonization by providing a more beneficial microenvironment for cell adhesion and growth. Furthermore, FGNs can also interact with cocultured cells by physical or chemical stimulation, which significantly mediate their cellular signaling and biological performance. In this review, we elucidate FGNs-bioorganism interactions and summarize recent advancements on designing FGN-based two-dimensional and three-dimensional architectures as multifunctional biological platforms. We have also discussed the representative biological applications regarding these FGN-based bioactive architectures. Furthermore, the future perspectives and emerging challenges will also be highlighted. Due to the lack of comprehensive reviews in this emerging field, this review may catch great interest and inspire many new opportunities across a broad range of disciplines.

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Section: Fgns–bioorganism Interactionsmentioning

confidence: 99%

Section: Fgns−mammalian Cell Toxicity and Interactionsmentioning

confidence: 99%

Functional Graphene Nanomaterials Based Architectures: Biointeractions, Fabrications, and Emerging Biological Applications

et al. 2017

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“…Membrane permeability can, e.g., be enhanced by conjugation to carbon allotropes, like single-walled carbon nanotubes (SWNT) or graphene oxide (GO). [ 90 – 96 ]…”

Section: Discussionmentioning

confidence: 99%

DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes

et al. 2016

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Extensive molecular-dynamics (MD) simulations have been used to investigate DNA-dye and DNA-photosensitizer conjugates, which act as reactants in templated reactions leading to the generation of fluorescent products in the presence of specific desoxyribonucleic acid sequences (targets). Such reactions are potentially suitable for detecting target nucleic acids in live cells by fluorescence microscopy or flow cytometry. The simulations show how the attached dyes/photosensitizers influence DNA structure and reveal the relative orientations of the chromophores with respect to each other. Our results will help to optimize the reactants for the templated reactions, especially length and structure of the spacers used to link reporter dyes or photosensitizers to the oligonucleotides responsible for target recognition. Furthermore, we demonstrate that the structural ensembles obtained from the simulations can be used to calculate steady-state UV-vis absorption and emission spectra. We also show how important quantities describing the quenching of the reporter dye via fluorescence resonance energy transfer (FRET) can be calculated from the simulation data, and we compare these for different relative chromophore geometries.

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“…In recent years, as the thinnest nanomaterial, graphene oxide (GO) has attracted more attention because of exceptional physical and chemical properties, including excellent biocompatibility and good thermal stability ( Joshi et al, 2015 ). Recent studies have reported the biomedical applications of GO, including drug delivery, biomedicine, and cancer diagnosis ( Kovbasyuk and Mokhir, 2016 ). Previous studies have demonstrated the antitumor effects of GO by delivering targeted chemotherapy drugs ( Hwang et al, 2017 ; Rosli et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Codelivery of Anticancer Drug and Photosensitizer by PEGylated Graphene Oxide and Cell Penetrating Peptide Enhanced Tumor-Suppressing Effect on Osteosarcoma

Zhang

Lan

et al. 2021

Front. Mol. Biosci.

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Objective: Graphene oxide (GO) has been widely used for various biological and biomedical applications due to its unique physiochemical properties. This study aimed to investigate the effects of cell penetrating peptide (CPP) modified and polyethylene-glycol- (PEG-) grafted GO (pGO) loaded with photosensitive agent 2-(1-hexyloxyethyl)-2-devinyl pyropheophorbide-alpha (HPPH) and Epirubicin (EPI) (HPPH/EPI/CPP-pGO) on tumor growth in osteosarcoma.Methods: The HPPH/EPI/CPP-pGO were prepared, and then in vitro drug release assay was conducted. The detection of singlet oxygen (1O2) and cellular uptake of HPPH was performed as well. Next, the effects of control (saline solution), CPP-pGO, EPI, HPPH, HPPH/CPP-pGO, EPI/CPP-pGO, HPPH/EPI/pGO, and HPPH/EPI/CPP-pGO were evaluated by MTT assay, colony-forming assay, and cell apoptosis assay in MG-63 cells. Furthermore, the antitumor effects of HPPH/EPI/CPP-pGO on osteosarcoma xenograft mice were unraveled.Results: The 1O2 generation and cellular uptake of HPPH were significantly increased after CPP and pGO modification compared with free HPPH. In addition, compared with control cells, CPP-pGO treatment had low cytotoxicity in MG-63 cells. Compared with free HPPH or EPI, HPPH/CPP-pGO or EPI/CPP-pGO treatment significantly inhibited cell viability and colony forming number, as well as inducing cell apoptosis. HPPH/EPI-pGO treatment showed stronger inhibition effects on MG-63 cells than HPPH/CPP-pGO or EPI/CPP-pGO, and HPPH/EPI/CPP-pGO was the most effective one. Similarly, in vivo experiments revealed that, compared with control group, the tumor size and weight of osteosarcoma xenograft mice were obviously decreased after free HPPH or EPI treatment, which were further reduced in other groups, especially in HPPH/EPI/CPP-pGO group.Conclusion: HPPH/EPI/CPP-pGO had superior tumor-inhibiting effects in vitro and in vivo on osteosarcoma.

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Toxicity Studies and Biomedical Applications of Graphene Oxide

Cited by 3 publications

References 71 publications

Functional Graphene Nanomaterials Based Architectures: Biointeractions, Fabrications, and Emerging Biological Applications

Functional Graphene Nanomaterials Based Architectures: Biointeractions, Fabrications, and Emerging Biological Applications

DNA-Dye-Conjugates: Conformations and Spectra of Fluorescence Probes

Codelivery of Anticancer Drug and Photosensitizer by PEGylated Graphene Oxide and Cell Penetrating Peptide Enhanced Tumor-Suppressing Effect on Osteosarcoma

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