Toxicity testing of herbicide norflurazon on an aquatic bioindicator species – the planarian Polycelis felina (Daly.)

Horvat, Tea; Kalafatić, Mirjana; Kopjar, Nevenka; Kovačević, Goran

doi:10.1016/j.aquatox.2005.03.023

Cited by 58 publications

(27 citation statements)

References 17 publications

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“…The genotoxic potential of water from Diluvio's Basin was evaluated in planarians where increase in pollutants towards the basin led to an increase in the DNA damage in these species (Prá et al 2005). Significant increase of primary DNA damage was observed in planarian cells due to Norflurazon, a bleaching herbicide (Horvat et al 2005), and copper sulfate (Guecheva et al 2001) when compared to the control animals.…”

Section: Comet Assay In Other Invertebratesmentioning

confidence: 99%

Comet assay: a reliable tool for the assessment of DNA damage in different models

2008

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New chemicals are being added each year to the existing burden of toxic substances in the environment. This has led to increased pollution of ecosystems as well as deterioration of the air, water, and soil quality. Excessive agricultural and industrial activities adversely affect biodiversity, threatening the survival of species in a particular habitat as well as posing disease risks to humans. Some of the chemicals, e.g., pesticides and heavy metals, may be genotoxic to the sentinel species and/or to non-target species, causing deleterious effects in somatic or germ cells. Test systems which help in hazard prediction and risk assessment are important to assess the genotoxic potential of chemicals before their release into the environment or commercial use as well as DNA damage in flora and fauna affected by contaminated/polluted habitats. The Comet assay has been widely accepted as a simple, sensitive, and rapid tool for assessing DNA damage and repair in individual eukaryotic as well as some prokaryotic cells, and has increasingly found application in diverse fields ranging from genetic toxicology to human epidemiology. This review is an attempt to comprehensively encase the use of Comet assay in different models from bacteria to man, employing diverse cell types to assess the DNA-damaging potential of chemicals and/or environmental conditions. Sentinel species are the first to be affected by adverse changes in their environment. Determination of DNA damage using the Comet assay in these indicator organisms would thus provide information about the genotoxic potential of their habitat at an early stage. This would allow for intervention strategies to be implemented for prevention or reduction of deleterious health effects in the sentinel species as well as in humans.

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Section: Comet Assay In Other Invertebratesmentioning

confidence: 99%

Comet assay: a reliable tool for the assessment of DNA damage in different models

2008

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“…Comet assay has become one of the most popular tools for detecting DNA stand breaks in aquatic animals (Frenzilli et al, 2009) and has been employed to assess the DNA damage in aquatic vertebrates such as Cyprinus carpio (Arabi, 2004), Carassius auratus (Masuda et al, 2004), Danio rerio (Kosmehl et al, 2008) and Liza aurata (Pereira et al, 2009), and invertebrates such as Limnoperna fortunei (Villela et al, 2007), Dreissena polymorpha (Juhel et al, 2007), Polycelis feline (Horvat et al, 2005), Palaemonetes pugio (Lee et al, 2000(Lee et al, , 2008, Mytilus edulis (Rank and Jensen, 2003;Cheung et al, 2006) and Crassostrea virginica (Bissett et al, 2009). For in vivo studies in aquatic invertebrates such as oysters, mussels, clams and shrimp, cells from haemolymph, embryos, gills and digestive glands were used for Comet assay (Lee and Steinert, 2003).…”

mentioning

confidence: 99%

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Jose

Puthumana

Mohandas

et al. 2011

Marine Environmental Research

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a b s t r a c tLack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos.Using MTT assay 12 h IC 50 values calculated were 31.09 AE 16.27 mM and 5.52 AE 1.16 mM for cadmium chloride and mercuric chloride and 59.94 AE 52.30 mg l À1 and 186.76 AE 77.00 mg l À1 for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.

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“…Horvat et al (2005) found that the toxicity of norflurazon caused mortality in Polycelis feline, and morphological and histological changes in treated animals compared to corresponding controls. The most prominent histological changes were damage of the outer mucous layer, lack of rhabdites, damage to epidermis and extensive damage to parenchyma cells.…”

Section: Lipid Synthesis Inhibitorsmentioning

confidence: 99%

Adverse Effects of Herbicides on Freshwater Zooplankton

Rico‐Martínez¹,

Arias-Almeida²,

Pérez‐Legaspi³

et al. 2012

Herbicides - Properties, Synthesis and Control of Weeds

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Toxicity testing of herbicide norflurazon on an aquatic bioindicator species – the planarian Polycelis felina (Daly.)

Cited by 58 publications

References 17 publications

Comet assay: a reliable tool for the assessment of DNA damage in different models

Comet assay: a reliable tool for the assessment of DNA damage in different models

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Adverse Effects of Herbicides on Freshwater Zooplankton

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