Toxicity to the pea aphid Acyrthosiphon pisum of anti-chymotrypsin isoforms and fragments of Bowman–Birk protease inhibitors from pea seeds

Multigene Family for Bowman–Birk Type Proteinase Inhibitors of Wild Soja and Soybean: The Presence of Two BBI-A Genes and Pseudogenes

“…[3][4][5] A number of functions have been proposed for BBIs, including the regulation of endogenous proteinases during germination, the storage of sulfur amino acids during dormancy, and the protection of the plant from insects and microorganisms, [6][7][8] while recent interest has focused on its medicinal utility as an antitumor agent, for instance.…”

Induction of Apoptosis by Buckwheat Trypsin Inhibitor in Chronic Myeloid Leukemia K562 Cells

“…PBMCs obtained from volunteers were grown in the above-mentioned culture medium. Cells (1ϫ10 6 ) were plated in 96-well microtiter plates and in 24-well microtiter plates, and incubated in medium containing various concentrations of rBTI for the indicated times. Following this, apoptosis was examined using different methods.…”

“…The K562 cells (1ϫ10 6 ) and normal human PBMCs (2ϫ10 5 ) were treated with rBTI (12.5-100 mg/ml) and were separately transferred to quadruplicate wells of 96-well microtiter plates at the indicated times. After incubating at 37°C for 24 h, 20 ml MTT was added for 4 h; following this, 80 ml DMSO was added.…”

“…4) It is known that proteinase inhibitors have multiple functions, including the regulation of endogenous proteases during germination and protection of plants from insects and microorganisms. [5][6][7] In recent years, it has been found that proteinase inhibitors can induce apoptosis of cancer cells in vitro; therefore, proteinase inhibitors have been receiving attention as potential anti-cancer agents. 8) It has been reported that the Bowman-Birk family of inhibitors, obtained from soybeans and other legumes, are potentially nutritionally relevant anti-carcinogens, particularly with respect to colon cancer.…”

Enhancement of tomato resistance to Tuta absoluta by the expression of two barley proteinase inhibitors

“…shown that the effect of PIs on insects is dose dependent, higher resistance is acquired when PIs are expressed at high levels (De Leo et al, 1998;Rahbé et al, 2003). Generating genetically engineered tomato plants expressing higher levels of BTI-CMe and Hv-CPI2 could inflict stronger harm to Tuta absoluta and provide a better control of its population.…”