Toxicity tolerance of Fusarium oxysporum towards inhibitory compounds formed during pretreatment of lignocellulosic materials

Xiros, Charilaos; Vafiadi, Christina; Paschos, Thomas; Christakopoulos, Paul

doi:10.1002/jctb.2499

Cited by 22 publications

(16 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Palmqvist et al (1996) reported that furfural is more inhibitory to S. cerevisiae fermentations than HMF. No toxicity levels have been reported so far for N. crassa, while Xiros et al (2011) observed that furfural had the most severe inhibitory effects than HMF on growth of the fungus Fusarium oxysporum F3 and ethanol fermentation is inhibited by 20-50 % at concentrations above 3.0 g/l furfural and 3.2 g/l HMF. Generally, acetic acid is inhibitory to yeast when its concentration is between 4 and 10 g/l (Cardona et al 2010).…”

Section: Discussionmentioning

confidence: 70%

Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa

Dogaris

Gkounta

Mamma

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Bioethanol production from sweet sorghum bagasse (SB), the lignocellulosic solid residue obtained after extraction of sugars from sorghum stalks, can further improve the energy yield of the crop. The aim of the present work was to evaluate a cost-efficient bioconversion of SB to ethanol at high solids loadings (16 % at pretreatment and 8 % at fermentation), low cellulase activities (1-7 FPU/g SB) and co-fermentation of hexoses and pentoses. The fungus Neurospora crassa DSM 1129 was used, which exhibits both depolymerase and co-fermentative ability, as well as mixed cultures with Saccharomyces cerevisiae 2541. A dilute-acid pretreatment (sulfuric acid 2 g/100 g SB; 210 °C; 10 min) was implemented, with high hemicellulose decomposition and low inhibitor formation. The bioconversion efficiency of N. crassa was superior to S. cerevisiae, while their mixed cultures had negative effect on ethanol production. Supplementing the in situ produced N. crassa cellulolytic system (1.0 FPU/g SB) with commercial cellulase and β-glucosidase mixture at low activity (6.0 FPU/g SB) increased ethanol production to 27.6 g/l or 84.7 % of theoretical yield (based on SB cellulose and hemicellulose sugar content). The combined dilute-acid pretreatment and bioconversion led to maximum cellulose and hemicellulose hydrolysis 73.3 % and 89.6 %, respectively.

show abstract

Section: Discussionmentioning

confidence: 70%

Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa

Dogaris

Gkounta

Mamma

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…The FPU activity of the fungus F. oxysporum F3 isolated from cumin achieved maximum values of 0.85 U/mL after 70 h of cultivation based on the corn cobs and brewers spent grain as carbon sources (Xiros et al, 2009). It was noteworthy that the toxicity tolerance of F. oxysporum F3 was high enough for aerobic growth in the presence of significant concentrations of inhibitors (Xiros et al, 2011). This might indicate the natural environmental-stress tolerance of the Fusarium genus.…”

Section: Isolation Of Novel Ionic Liquids-tolerant Strain Of Cellulolmentioning

confidence: 96%

A novel ionic liquid-tolerant Fusarium oxysporum BN secreting ionic liquid-stable cellulase: Consolidated bioprocessing of pretreated lignocellulose containing residual ionic liquid

Wang²,

et al. 2015

Bioresource Technology

View full text Add to dashboard Cite

“…With respect to toxic compounds during ethanol production [23], what is sought is to have the optimum conditions in which there is no generation of these compounds, or at least not existing in relevant concentrations, by which it was proposed to work at 100 ∘ C and 1% acid concentrations, where F is not generated and HMF is generated below 2.98%. It has been shown that F concentrations above 3 g/L of and 3.2 g/L HMF inhibit bioethanol fermentation by 20 and 50%, respectively [24]. Therefore, it can be said that 2.98% 5-HMF does not affect the next steps in the fermentation process for bioethanol production.…”

Section: Generation Of Toxic Compounds Derived From Furfuralmentioning

confidence: 99%

Study of Chemical and Enzymatic Hydrolysis of Cellulosic Material to Obtain Fermentable Sugars

Amezcua‐Allieri

Durán

Aburto

2017

Journal of Chemistry

View full text Add to dashboard Cite

The objective of this study was to evaluate the chemical and enzymatic hydrolysis using a factorial experimental design (2 3 ) in order to obtain fermentable sugars from cellulose-based material (CBM) usually used as pet litter. In assessing chemical hydrolysis, we studied the effect of temperature, in addition to H 2 SO 4 concentration and reaction time, on the production of total sugars, reducing sugars, soluble lignin, carbohydrate profile, furfural (F), and hydroxymethyl furfural (HMF). We performed a response surface analysis and found that, at 100 ∘ C, 1% acid concentration, and 60 min reaction time, the yields of 0.0033 g reducing sugar/g biomass and 0.0852 g total sugars/g biomass were obtained. Under the above conditions, F is not generated, while HMF is generated in such a concentration that does not inhibit fermentation. We pretreated the CBM with H 2 SO 4 , NaOH, CaO, or ozonolysis, in order to evaluate the effectiveness of the enzymatic hydrolysis from the pretreated biomass, using an enzymatic cocktail. Results showed that CBM with acid was susceptible to enzymatic attack, obtaining a concentration of 0.1570 g reducing sugars/g biomass and 0.3798 g total sugars/g biomass. We concluded that acid pretreatment was the best to obtain fermentable sugars from CBM.

show abstract

Toxicity tolerance of Fusarium oxysporum towards inhibitory compounds formed during pretreatment of lignocellulosic materials

Cited by 22 publications

References 23 publications

Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa

Bioconversion of dilute-acid pretreated sorghum bagasse to ethanol by Neurospora crassa

A novel ionic liquid-tolerant Fusarium oxysporum BN secreting ionic liquid-stable cellulase: Consolidated bioprocessing of pretreated lignocellulose containing residual ionic liquid

Study of Chemical and Enzymatic Hydrolysis of Cellulosic Material to Obtain Fermentable Sugars

Contact Info

Product

Resources

About