Toxicological and Genotoxicological evaluation of Chitosan Nanoparticles Loaded with Pinene in A Murine Model

Abstract

Byrsonima crassifolia (Malpighiaceae) has been widely used in Mexican traditional medicine as a remedy for the treatment of depression and diabetes. Various monoterpenes and diterpenes, have been identified from the active extract and has been demonstrated their antifungal activity. The α-pinene is present mostly and was loaded in chitosan Nanoparticles (P-CSNP) by the nanoprecipitation method. The morphology, particle size distribution, Zeta potential, the identification of the functional groups presents in P-CSNP, the toxicity and genotoxicity were evaluated. The nanoparticles and nanoparticles based coatings (ECP-CSNP) were administering to mice for 28 days (2.5 mg/g). Show spherical shape nanoparticles with a particle size of 5 ± .07nm. The P-CSNP showed a binomial distribution in which 81.5% of its population had a size of 3.9 ± 0.5 nm and a Z potential of 13.4 to 14.9 mV. The P-CSNP showed similarities between the patterns of the FTIR spectra suggest the union of the α-pinene aromatic groups and the nanometric Toxicological … Gonzalo Hernández López et al.