Toxicological Assessment of Ammonia Exposure on Carassius auratus red var. Living in Landscape Waters

Hao, Ming; Zuo, Qiting; Zhang, Wei; Feng, Yakun; Wang, Li; Yu, Luji; Zhang, Xu; Li, Jing; Huang, Zehan

doi:10.1007/s00128-019-02728-5

Cited by 7 publications

(2 citation statements)

References 35 publications

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“…Our current study found that ammonia decreased the GSH content in fish macrophages. Similar results were also detected in the hepatopancreas of Carassius auratus red var (Hao et al, 2019) and juvenile turbot (Scophthalmus maximus) (Jia et al, 2017). Thus, we speculated that ammonia stress might induce GSH depletion to interfere the normal function of GPX4, resulting in the elevation of ROS level in fish macrophages.…”

Section: Ta B L E 1 Real-time Pcr Primer Sequencessupporting

confidence: 77%

Ammonia triggers ferroptosis in macrophages of yellow catfish Pelteobagrus fulvidraco

Gan

Yao

Zhang

et al. 2021

Aquaculture Research

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An in vitro experiment was carried out to investigate the effect of ammonia on the macrophages of yellow catfish Pelteobagrus fulvidraco from a ferroptotic point of view.The fish macrophages were cultured with 0.00, 0.12, 0.23 and 0.46 mg/L ammonia for 24 h. The result showed that ammonia decreased the cell viability, reduced glutathione (GSH) contents and increased the reactive oxygen species (ROS) levels of fish macrophages. Meanwhile, the mRNA levels of systemX − c and ferritin light chain1 (FTL1) were down-regulated when ammonia concentration was elevated up to 0.12 mg/L.Compared with control, the mRNA level of glutathione peroxidase 4 (GPX4) was upregulated when ammonia was elevated up to 0.12 mg/L, but was down-regulated when ammonia reached 0.46 mg/L. Compared with control, the mRNA level of lipoxygenase (LOX)-12 was up-regulated when ammonia was elevated up to 0.12 mg/L, but was down-regulated when ammonia reached 0.23 mg/L, and the mRNA levels of LOX-5, LOX-15b and transferrin Receptor 1 (TFR1) were up-regulated when ammonia was elevated up to 0.12 mg/L, but was down-regulated when ammonia reached 0.23 mg/L. In conclusion, our study found that ammonia could trigger ferroptosis in the macrophage of fish.

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Section: Ta B L E 1 Real-time Pcr Primer Sequencessupporting

confidence: 77%

Ammonia triggers ferroptosis in macrophages of yellow catfish Pelteobagrus fulvidraco

Gan

Yao

Zhang

et al. 2021

Aquaculture Research

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“…The current study's findings matched those of Zhao et al [28], who found that antioxidant response induced by ammonia exposure led to elevated superoxide dismutase and catalase activities in the liver of Micropterus salmoides Kim et al [29] reported a substantial rise in superoxide dismutase activity in the livers of juvenile hybrid groupers exposed to varied sublethal amounts of ammonia (1, 2, 4, and 8 mg/L). In addition, glutathione, an important cellular antioxidant, also scavenges ROS to protect cells from oxidative stress [30]. The glutathione levels in large yellow croaker livers increased considerably following ammonia exposure, indicating that the fish avoided the impacts of oxidative stress on the organism by increasing glutathione levels.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Acute Exposure to Ammonia on Oxidative Stress, Hematological Parameters, Flesh Quality, and Gill Morphological Changes of the Large Yellow Croaker (Larimichthys crocea)

Guo

Zhang

et al. 2023

Animals

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Ammonia is considered to be the major chemical pollutant causing fish poisoning in aquaculture. This research aimed to evaluate the impact of acute ammonia exposure on the large yellow croaker’s meat quality, gill morphology, liver oxidative stress, and hematological parameters. The fish were exposed to total ammonia nitrogen concentrations of 0, 2.96, 5.92, and 8.87 mg/L for 48 h, respectively. The findings demonstrated that all ammonia-exposed fish had higher liver lactate dehydrogenase and glutamic oxalate transaminase activities. The glucose, blood urea nitrogen, and creatinine levels in 8.87 mg/L total ammonia nitrogen (TAN) were higher than other samples. The total protein, albumin, and triglyceride levels in serum decreased significantly in ammonia-exposed samples. After 48 h of ammonia exposure, superoxide dismutase activities showed a 76.1%, 118.0%, and 156.8% increase when fish were exposed to 2.96, 5.92, and 8.87 mg/L TAN, respectively. Catalase activities and glutathione contents were considerably higher (p < 0.05) in all ammonia-treated samples compared to 0 mg/L TAN. The ammonia-treated gill lamellae become thicker, shorter, and curved. Additionally, the ammonia exposure resulted in the accumulation of free amino acids and the loss of nucleotides. The inosine monophosphate and adenosine monophosphate contents in the flesh were decreased after 12 h of exposure to 2.96, 5.92, and 8.87 mg/L ammonia compared to the control group. Overall, large yellow croakers exposed to ammonia for 6 h presented not only changes in serum composition but also oxidative stress, liver and gill tissue damage and flesh quality deterioration.

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Toxicological Assessment of Ammonia Exposure on Carassius auratus red var. Living in Landscape Waters

Cited by 7 publications

References 35 publications

Ammonia triggers ferroptosis in macrophages of yellow catfish Pelteobagrus fulvidraco

Ammonia triggers ferroptosis in macrophages of yellow catfish Pelteobagrus fulvidraco

The Effects of Acute Exposure to Ammonia on Oxidative Stress, Hematological Parameters, Flesh Quality, and Gill Morphological Changes of the Large Yellow Croaker (Larimichthys crocea)

Ameliorative effect of gamma-aminobutyric acid on the antioxidant status and ammonia stress resistance of Micropterus salmoides

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