Toxicological Characterization of Six Plants of the Beninese Pharmacopoeia Used in the Treatment of Salmonellosis

Lègba, Boris; Dougnon, Victorien; Déguénon, Esther; Agbankpé, Alidehou Jerrold; Sènou, Maximin; Aniambossou, Alidah; Gbaguidi, Carène; Sintondji, Kévin; Baba-Moussa, Lamine; Dougnon, Jacques

doi:10.1155/2019/3530659

Cited by 12 publications

(12 citation statements)

References 24 publications

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“…The results of the present study have shown that the 80% methanol leaves extracts of B. corieacea and U. leptocladon was found to be safe in the Swiss albino mice tested. The safety profile of B. coriacea and U. leptocladon leaves extracts noted in this study is comparable with other studies that evaluated toxicology of other Uvaria and Boscia species ( 22 – 24 ).…”

Section: Discussionsupporting

confidence: 87%

Toxicity study and antibacterial effects of the leaves extracts of Boscia coriacea and Uvaria leptocladon

Tseha¹,

Mekonnen²,

Desalegn³

et al. 2022

Ethiop J Health Sci

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BACKGROUND: The objective of this study was to evaluate the in vivo toxicity and antibacterial activity of the leaves extracts of Boscia coriacea and Uvaria leptocladon.METHODS: Extraction was performed using 80% methanol by maceration and Soxhlet extraction method. Evaluation of the acute toxicity of the extracts was based on the Organization for Economic Cooperation and Development (OECD) guideline. Evaluation of antibacterial activity of the extracts was done by agar well diffusion assay. Determinations of minimum inhibitory concentrations (MIC) of the extracts were performed by broth macro-dilution method. The checkerboard method was used for the determination of combined effect of antibiotics and the extracts.Paired T-test and one way analysis of variance were used for statistical analysis.RESULTS: B. coriacea and U. leptocladon have no toxic effect in Swiss albino mice up to dose of 5000 mg/kg. B. coriacea and U. leptocladon showed antibacterial activity at concentration of 500 mg/ml. The chloroform-methanol fraction of B. coriacea and U. leptocladon showed the highest antibacterial activity at concentration of 25 mg/ml. The MIC and minimum bactericidal concentration (MBC) of B. coriacea were 125 mg/ml and 250mg/ml, respectively. The MIC of U. leptocladon ranged from 31.25 mg/ml to 62.5 mg/ml, while its MBC ranged from 62.5 to 125 mg/ml. The combination assay of B. coriacea and the antibiotics showed additive effect, while U. leptocladon and the antibiotics showed indifferent effect.CONCLUSION: The findings showed that U. leptocladon and B. coriacea leaves extracts have antibacterial activity and no toxicity in animal model.

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Section: Discussionsupporting

confidence: 87%

Toxicity study and antibacterial effects of the leaves extracts of Boscia coriacea and Uvaria leptocladon

Tseha¹,

Mekonnen²,

Desalegn³

et al. 2022

Ethiop J Health Sci

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“…In the review by Zhao et al [40], R. tomentosa is considered as weak cytotoxicity and its IC 50 value of the ethanol extract was 15-fold higher than the MIC 90 value. In the acute toxicity test, a previous study showed that the aqueous extracts of P. guajava and P. amarus leaves did not induce any mortality or any histological renal and fetal disorders of the treated rats [21]. Using the brine shrimp lethality test, Gyawali et al [41] showed that the crude methanolic extract of the S. wallichii bark was mildly toxic and it was suggested to use for human consumption for drug purposes.…”

Section: Discussionmentioning

confidence: 99%

“…Researchers have also focused on the potential usages of the traditional medicinal plants for the treatment of some important infectious diseases such as tuberculosis, salmonellosis, or other food poisoning diseases [21][22][23]. In the customary practice, indigenous people use either fresh or dry plant materials to make the medicinal preparations.…”

Section: Introductionmentioning

confidence: 99%

Thermal and gastric stability of antimicrobial activity of juices and aqueous extracts prepared from common eligible herbs and traditional medicinal plants against Burkholderia pseudomallei and other enteric bacteria

Trung

Bui

et al. 2022

Futur J Pharm Sci

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Background Burkholderia pseudomallei is a causative agent of melioidosis, a fatal infectious disease highly prevalent in the tropics where traditional medicinal plants are widely used for the treatment of various human ailments. In this study, we aimed to evaluate the in vitro antibacterial activity of common eligible herbs and medicinal plants against B. pseudomallei. Thermal and gastric stability, antibacterial spectrum, bactericidal activity, and cell cytotoxicity were also tested to verify the possible usage of these plants in the treatment of melioidosis. Results Eighteen eligible herbs and twenty-one medicinal plants were collected. Herb juices and aqueous plant samples extracted at different temperatures were prepared for antibacterial testing. A higher proportion of aqueous plant extracts (17/21; 80.9%) against B. pseudomallei was observed, in comparison with that of herb juices (8/18; 44.5%). Two herb juices and twelve aqueous plant extracts were selected for further tests. The juices of A. sativum and A. tuberosum decreased their antimicrobial activity when treated at higher temperatures whereas the aqueous plant extracts increased their antimicrobial activity when prepared at 70 and 100 °C. The herb juices showed a broader spectrum of antimicrobial activity than the aqueous plant extracts. All samples showed less cytotoxicity on the HT29, HepG2, and HEK293 cell lines. At the 2× minimal inhibitory concentration (MIC), aqueous extracts of Blechnum orientale, Breynia fruticose, Psidium guajava, Rhodomyrtus tomentosa, Rosa odorata, and Schima wallichii showed similar bactericidal activity to that of amoxicillin clavulanic acid. The antimicrobial activity of Mangifera indica, Punica granatum, and R. tomentosa remained under the stimulated gastric conditions. Conclusion Our data indicate that traditional medicinal plants prepared by decoction could be effectively used to treat melioidosis via the oral route. Further in vivo investigations are needed to explore other alternative therapies for the prevention and treatment of tested pathogenic bacterial species.

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“…The study of Nkoua Badzi et al [ 38 ] reached the same conclusion with regard to the aqueous extract of Rauvolfia vomitoria. Legba et al [ 66 ] showed the safety of aqueous and ethanolic extracts of the leaves of Vernonia amygdalina used in Southern Benin. Ojo et al [ 40 ] and Satish and Ranjana [ 67 ] reported the safety of ethanolic aqueous extracts of the leaves of Manihot esculenta and Ocimum gratissimum , respectively.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Characterization of Ten Medicinal Plants of the Beninese Flora Used in the Traditional Treatment of Diarrheal Diseases

Dougnon

Hounsa

Agbodjento

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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The use of medicinal plants in traditional medicine is a common practice in developing countries. However, this unregulated or poorly rational use may present a dose-dependent risk of toxicity to humans. This study aimed to explore the phytochemical and toxicological characteristics of ten (10) plant species used in the traditional treatment of infectious diarrhea in Benin. The acute toxicity of aqueous and hydroethanolic extracts of Khaya senegalensis, Daniellia oliveri, Rauvolfia vomitoria, Vernonia amygdalina, Manihot esculenta, Ocimum gratissimum, Senna italica, Diospyros mespiliformis, Pterocarpus erinaceus, and Anacardium occidentale was evaluated following the OECD 423 protocol at a single dose of 2000 mg/kg. This safety test was complemented by a larval cytotoxicity test. Hematological and biochemical examinations, as well as a histological study of the liver and kidneys, were performed. Larval cytotoxicity was assessed by the sensitivity of Artemia salina larvae to different concentrations of the plant extracts studied. Testing for chemical compounds was performed on the basis of differential staining and precipitation reactions. The mean lethal concentration (LC50) was determined by the probit method. The qualitative phytochemical screening of the plants studied revealed the presence of catechic tannins, gallic tannins, flavonoids, anthocyanins and sterol-terpenes, alkaloids, saponosides, and reducing compounds. This composition varied according to the plants studied. Acute toxicity data indicated that there was no mortality and no structural and functional alterations of the liver and kidneys of treated animals. Larval cytotoxicity data suggest that the plants studied are not cytotoxic (LC50 ≥ 0.1 mg/mL). These observations reflect the safety of these plants and justify their use in traditional medicine in the treatment of many diseases including diarrheal diseases.

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Toxicological Characterization of Six Plants of the Beninese Pharmacopoeia Used in the Treatment of Salmonellosis

Cited by 12 publications

References 24 publications

Toxicity study and antibacterial effects of the leaves extracts of Boscia coriacea and Uvaria leptocladon

Toxicity study and antibacterial effects of the leaves extracts of Boscia coriacea and Uvaria leptocladon

Thermal and gastric stability of antimicrobial activity of juices and aqueous extracts prepared from common eligible herbs and traditional medicinal plants against Burkholderia pseudomallei and other enteric bacteria

Toxicological Characterization of Ten Medicinal Plants of the Beninese Flora Used in the Traditional Treatment of Diarrheal Diseases

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