Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model

Czekala, Lukasz; Simms, Liam; Stevenson, Matthew; Tschierske, Nicole; Maione, Anna G.; Walele, Tanvir

doi:10.1016/j.yrtph.2019.01.036

Cited by 70 publications

(65 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These findings are consistent with previous assessments of e-cig products by our group and others, where reduced biological activity was reported in comparison to that induced by cigarette smoke [ [6] , [7] , [8] , 12 , 29 , 46 ]. It is likely that the biological responses that are seen upon exposure to cigarette smoke may be driven by multiple toxic constituents of that aerosol, and that the specific constituents that drive the response may differ from one biological endpoint to the other.…”

Section: Discussionsupporting

confidence: 93%

“…In the interim, data from analytical chemistry of aerosol emissions, as well as preclinical and clinical assessment of the effects of exposure to these aerosols, will greatly enhance our understanding of the potential for these products to be less risky to the consumer. In vitro data suggest that e-cig aerosol exposure has reduced toxicological effects on lung [ [5] , [6] , [7] , [8] ] and cardiovascular cells [ 9 , 10 ], as well as inducing little to no activity in traditional genetic toxicology assessments [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The in vitro assessment of a novel vaping technology

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

The in vitro assessment of a novel vaping technology

et al. 2020

View full text Add to dashboard Cite

show abstract

“…This can include other in vitro human-based assays such as 3D lung models and high content screening. 55,56…”

Section: Productmentioning

confidence: 99%

Chemical Composition and In Vitro Toxicity Profile of a Pod-Based E-Cigarette Aerosol Compared to Cigarette Smoke

Rudd

Stevenson

Wieczorek

et al. 2020

Applied In Vitro Toxicology

Self Cite

View full text Add to dashboard Cite

Introduction: Electronic cigarette (e-cigarette) aerosol is understood to provide reduced exposure to harmful toxicants compared with tobacco cigarette smoke, as it delivers nicotine and flavors without the use of tobacco. Published studies have shown that e-cigarette aerosol is chemically simple compared with tobacco smoke and corresponding reductions in toxicity in vitro have been demonstrated. However, comprehensive analytical and in vitro assessments of many widely available and currently marketed products, including pod-based systems, are limited. Materials and Methods: Here we report comparative data for aerosol emissions and in vitro toxicity, using the neutral red uptake, the bacterial reverse mutation, and in vitro micronucleus assays, for a pod system e-cigarette compared with 3R4F reference cigarette smoke. Results and Discussion: Many of the harmful and potentially harmful constituents found in cigarette smoke were not detected in e-cigarette aerosol. Using established in vitro biological tests, e-cigarette aerosol did not display any mutagenic or genotoxic activity under the conditions of test. By contrast, 3R4F cigarette smoke displayed mutagenic and genotoxic activity. E-cigarette aerosol was also found to be *300-fold less cytotoxic than cigarette smoke in the neutral red uptake assay. Conclusion: Data presented here show clear differences between a tobacco cigarette reference product and a commercially available nontobacco containing e-cigarette product in terms of emissions and in vitro toxicity profile. Our results demonstrate that high-quality e-cigarettes and e-liquids may offer the potential for substantially reduced exposure to cigarette toxicants in adult smokers who use such products as alternatives to cigarettes.

show abstract

“…In addition, while exposure to cigarette smoke was found to be closely connected with dysregulated expression of numerous genes in NHBE cells, including those encoding proteins taking part in oxidative stress and cell death processes, exposure to e-cigarette vapor elicited minimal modulation of gene expression [199]. Additionally, such findings indicating the lower harm of e-cigarette aerosol has been confirmed in human respiratory 3D tissue models [200]. Another study on human volunteers suggests that e-cigarette smoking is significantly less harmful to lung physiology and may be recommended for tobacco smoking cessation [201].…”

Section: E-cigarette Vapor Vs Cigarette Smokementioning

confidence: 88%

A Summary of In Vitro and In Vivo Studies Evaluating the Impact of E-Cigarette Exposure on Living Organisms and the Environment

Merecz-Sadowska

Sitarek

Zielińska‐Bliźniewska

et al. 2020

IJMS

View full text Add to dashboard Cite

Worldwide use of electronic cigarettes has been rapidly expanding over recent years, but the long-term effect of e-cigarette vapor exposure on human health and environment is not well established; however, its mechanism of action entails the production of reactive oxygen species and trace metals, and the exacerbation of inflammation, which are associated with potential cytotoxicity and genotoxicity. The present study examines the effects of selected liquid chemicals used in e-cigarettes, such as propylene glycol/vegetable glycerin, nicotine and flavorings, on living organisms; the data collected indicates that exposure to e-cigarette liquid has potentially detrimental effects on cells in vitro, and on animals and humans in vivo. While e-liquid exposure can adversely influence the physiology of living organisms, vaping is recommended as an alternative for tobacco smoking. The study also compares the impact of e-cigarette liquid exposure and traditional cigarette smoke on organisms and the environmental impact. The environmental influence of e-cigarette use is closely connected with the emission of airborne particulate matter, suggesting the possibility of passive smoking. The obtained data provides an insight into the impact of nicotine delivery systems on living organisms and the environment.

show abstract

Toxicological comparison of cigarette smoke and e-cigarette aerosol using a 3D in vitro human respiratory model

Cited by 70 publications

References 64 publications

The in vitro assessment of a novel vaping technology

The in vitro assessment of a novel vaping technology

Chemical Composition and In Vitro Toxicity Profile of a Pod-Based E-Cigarette Aerosol Compared to Cigarette Smoke

A Summary of In Vitro and In Vivo Studies Evaluating the Impact of E-Cigarette Exposure on Living Organisms and the Environment

Contact Info

Product

Resources

About