Toxicological effects of As (V) in juvenile rockfish Sebastes schlegelii by a combined metabolomic and proteomic approach

Abstract

Arsenic (As) is a metalloid element that is ubiquitous in the marine environment and its contamination has received worldwide attention due to its potential toxicity. Arsenic can induce multiple adverse effects, such as lipid metabolism disorder, immune system dysfunction, oxidative stress and carcinogenesis, in animals. Inorganic arsenic includes two chemical forms, arsenite (As (III)) and arsenate (As (V)), in natural environment. As (V) is the dominant form in natural waters. In the present study, metabolomic and proteomic alterations were investigated in juvenile rockfish Sebastes schlegelii exposed to environmentally relevant concentrations of As (V) for 14 d. The analysis of iTRAQ-based proteomics combined with untargeted NMR-based metabolomics indicated apparent toxicological effects induced by As (V) in juvenile rockfish. In details, the metabolites, including lactate, alanine, ATP, inosine and phosphocholine were significantly altered in As-treated groups. Proteomic responses suggested that As (V) could not only affected energy and primary metabolisms and signal transduction, but also influenced cytoskeleton structure in juvenile rockfish. This work suggested that the combined proteomic and metabolomic approach could shed light on the toxicological effects of pollutants in rockfish S. schlegelii.

Toxicological effects of cadmium on deep-sea mussel Gigantidas platifrons revealed by a combined proteomic and metabolomic approach

“…Cytoskeletal injuries in bivalves induced by metal pollution have been repeatedly confirmed (Gomes et al, 2013;Xu et al, 2016). And these cytoskeletal-related DEPs are generally contain actins, tubulins, and myosins in marine bivalves (Xu et al, 2019), a finding consist with our study's results. Consequently, the significantly altered cytoskeletal proteins demonstrate that oxidative stress and subsequent disturbed cytoskeleton and cell structures can be induced by Cd in deepsea mussels.…”

“…In the high-dose group, however, we found that NDUFA5 was unaltered, but all the proteins associated with oxidative phosphorylation (mitochondrial ATP synthase subunit d precursor and V-type proton ATPase 16 kDa proteolipid subunit) were up-regulated and related to ATP synthase, implying an increased demand for ATP production in deep-sea mussels. Increased oxidative phosphorylation was reported in the rock fish Sebastes schlegelii under arsenic exposure and the mussel Mytilus galloprovincialis under nano-Ag exposure, both revealing a process of energetic re-adjustment to counteract the effects of arsenic toxicity (Gomes et al, 2013;Xu et al, 2019). In particular, to compensate for the diminished energy supply caused by the inhibition of glycolysis and the TCA, deep-sea mussels bolstered amino acid levels in their body to protect themselves from more damage caused by high-dose Cd exposure (Table 2).…”

“…Recently, with dramatic advances in high-throughput molecular tools, 'omic' technologies including proteomics and metabolomics have been extensively used in toxicological studies of marine environments (Sanchez et al, 2011;Xu et al, 2019). Proteomics theoretically characterizes the entire protein pool at the organelle, cellular or tissue level, and is capable of detecting significantly different expressed proteins (DEPs) caused by exogenous factors in organisms (Tomanek, 2011;Campos et al, 2012).…”

1H-NMR metabolomics profiling of zebra mussel (Dreissena polymorpha): A field-scale monitoring tool in ecotoxicological studies

“…Metabolomic studies can be performed using different analytical techniques, such as mass spectrometry (MS) (Courant et al, 2014) and nuclear magnetic resonance spectroscopy (NMR) (Watanabe et al, 2015). Proton nuclear magnetic resonance ( 1 H-NMR) spectroscopybased metabolomics is a commonly used tool for studying the effects of pollutants on the metabolic profiles of aquatic organisms, in laboratory or field-scale conditions (Cappello et al, 2017(Cappello et al, , 2016(Cappello et al, , 2013Jones et al, 2008;Kwon et al, 2012;Maisano et al, 2017;Tuffnail et al, 2009;Xu et al, 2019). Because the spectral peak areas are quantitatively related to metabolite concentrations, this technique simultaneously provides unbiased and reproducible data about a wide range of metabolites.…”

Molecular biological methods in environmental engineering

“…The effects of metal pollutants on marine organisms can be studied by combining metabolomics and proteomics. As (V) not only affects metabolism and signal transduction of juvenile rockfish Sebastes schlegelii ., but also affects its cytoskeleton structure (Xu et al, 2019).…”