Toxicological effects of cinnabar in rats by NMR-based metabolic profiling of urine and serum

Wei, Lai; Liao, Peiqiu; Wu, Huifeng; Li, Xiaojing; Pei, Fengkui; Li, Weisheng; Wu, Yidi

doi:10.1016/j.taap.2007.11.015

Cited by 111 publications

(80 citation statements)

References 55 publications

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“…In addition, the biological differences can also distort biological interpretation due to the different tolerances and sensitivities of clams to environmental contaminants in marine toxicology studies. As a system biology approach, metabolomics has been widely used in toxicology Wei et al, 2008;Wei et al, 2009;Williams et al, 2009Williams et al, , 2014Zhang et al, 2006;).…”

Section: Biological Differences Between White and Zebra Clamsmentioning

confidence: 99%

Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics

Cao

2015

Environmental Toxicology and Pharmacology

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EcotoxicologyHeavy metals Metabolomics NMR a b s t r a c t Heavy metal pollution has been of great concern in the Bohai marine environment. Manila clam Ruditapes philippinarum has been used as a bioindicator in marine toxicology. In this study, NMR-based metabolomics was used to ascertain whether there were significant biological differences between two dominant pedigrees (White and Zebra) of clam and evaluate the suitability of two pedigrees for marine environmental toxicology, together with antioxidant enzymatic analysis. Our results indicated that there were significant biological differences between White and Zebra clams based on the metabolic profiles and antioxidant enzyme activities. In details, the metabolic profiles showed higher levels of amino acids and succinate in Zebra clam digestive glands and higher levels of ATP in White clam digestive glands, respectively. The superoxide dismutase activities in control White and Zebra clam samples were significantly different. Additionally, White clam was more sensitive to Cd based on the significant accumulation of Cd, antioxidant enzymatic alterations and sensitive metabolic changes. Overall, we concluded that White clam could be a preferable bioindicator for marine environmental toxicology.

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Section: Biological Differences Between White and Zebra Clamsmentioning

confidence: 99%

Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics

Cao

2015

Environmental Toxicology and Pharmacology

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“…(Liao et al 2007;Wei et al 2008;Wang et al 2006;Waters et al 2006). It is well established that nuclear magnetic resonance (NMR) technique, coupled with pattern recognition (PR) methods, can provide valuable information on biochemical perturbations due to both exogenous and endogenous factors by analysis of biofluids (Waters et al 2006;Holmes et al 2008).…”

Section: Introductionmentioning

confidence: 99%

NMR spectroscopy based metabolic profiling of urine and serum for investigation of physiological perturbations during radiation sickness

et al. 2011

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Radiation accidents are rare events that induce radiation syndrome, a complex pathology which is difficult to treat. In medical management of radiation victims, life threatening damage to different physiological systems should be taken into consideration. The present study was proposed to identify metabolic and physiological perturbations in biofluids of mice during different phases of radiation sickness using 1 H nuclear magnetic resonance ( 1 H NMR) spectroscopy and pattern recognition (PR) technique. The 1 H NMR spectra of the biofluids collected from mice irradiated with 5 Gray (Gy) at different time points during radiation sickness were analysed visually and by principal components analysis. Urine and serum spectral profile clearly showed altered metabolic profiles during different phases of radiation sickness. Increased concentration of urine metabolites viz. citrate, a ketoglutarate, succinate, hippurate, and trimethylamine during prodromal and clinical manifestation phase of radiation sickness shows altered gut microflora and energy metabolism. On the other hand, serum nuclear magnetic resonance (NMR) spectra reflected changes associated with lipid, energy and membrane metabolism during radiation sickness. The metabonomic time trajectory based on PR analysis of 1 H NMR spectra of urine illustrates clear separation of irradiated mice group at different time points from pre dose. The difference in NMR spectral profiles depicts the pathophysiological changes and metabolic disturbances observed during different phases of radiation sickness, that in turn, demonstrate involvement of multiple organ dysfunction. This could further be useful in development of multiparametric approach for better evaluation of radiation damage as well as for medical management during radiation sickness.

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“…Most creatine is phosphorylated by creatine kinase to form phosphocreatine in a reversible reaction that acts as an energy buffer to maintain ATP homeostasis [20,21]. Approximately 2 % of creatine and phosphocreatine is nonenzymatically converted to creatinine [22].…”

Section: Long-term Microwave Exposure Induced Energy Metabolism Distumentioning

confidence: 99%

Application of 1H-NMR-based metabolomics for detecting injury induced by long-term microwave exposure in Wistar rats’ urine

Wang

Peng

et al. 2012

Anal Bioanal Chem

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There has been growing public concern regarding exposure to microwave fields as a potential human health hazard. This study aimed to identify sensitive biochemical indexes for the detection of injury induced by microwave exposure. Male Wistar rats were exposed to microwaves for 6 min per day, 5 days per week over a period of 1 month at an average power density of 5 mW/cm(2) (specific absorption rate of 2.1 W/kg). Urine specimens were collected over 24 h in metabolic cages at 7 days, 21 days, 2 months, and 6 months after exposure. (1)H NMR spectroscopy data were analyzed using multivariate statistical techniques. Urine metabolic profiles of rats after long-term microwave exposure were significantly differentiated from those of sham-treated controls using principal component analysis or partial least squares discriminant analysis. Significant differences in low molecular weight metabolites (acetate, succinate, citrate, ketoglutarate, glucose, taurine, phenylalanine, tyrosine, and hippurate) were identified in the 5 mW/cm(2) microwave exposure group compared with the sham-treated controls at 7 days, 21 days, and 2 months. Metabolites returned to normal levels by 6 months after exposure. These data indicated that these metabolites were related to the perturbations of energy metabolism particularly in the tricarboxylic acid cycle, and the metabolism of amino acids, monoamines, and choline in urine represent potential indexes for the detection of injury induced by long-term microwave exposure.

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Toxicological effects of cinnabar in rats by NMR-based metabolic profiling of urine and serum

Cited by 111 publications

References 55 publications

Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics

Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics

NMR spectroscopy based metabolic profiling of urine and serum for investigation of physiological perturbations during radiation sickness

Application of 1H-NMR-based metabolomics for detecting injury induced by long-term microwave exposure in Wistar rats’ urine

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