Toxicological effects of nanometer titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii

Chen, Lanzhou; Zhou, Lina; Liu, Yongding; Deng, Songqiang; Wu, Hao; Wang, Gaohong

doi:10.1016/j.ecoenv.2012.07.019

Cited by 163 publications

(74 citation statements)

References 47 publications

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“…With the increasing use of engineered NPs, there is an enormous uncertainty with respect to their potential environmental impacts, including their toxicological effects. Of special concern is the emerging evidence that numerous NPs can produce reactive oxygen species (ROS), release toxic metals, or react directly with the biological membrane (1,2). A major key question is whether toxicological effects are due to general properties shared by diverse NP types or whether they are specific to each NP.…”

mentioning

confidence: 99%

Transcriptome Sequencing (RNA-seq) Analysis of the Effects of Metal Nanoparticle Exposure on the Transcriptome of Chlamydomonas reinhardtii

Simon

Domingos

Hauser

et al. 2013

Appl Environ Microbiol

117

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The widespread use of nanoparticles (NPs) raises concern over their potential toxicological effects in humans and ecosystems. Here we used transcriptome sequencing (RNA-seq) to evaluate the effects of exposure to four different metal-based NPs, nano-Ag (nAg), nano-TiO 2 (nTiO 2 ), nano-ZnO (nZnO), and CdTe/CdS quantum dots (QDs), in the eukaryotic green alga Chlamydomonas reinhardtii. The transcriptome was characterized before and after exposure to each NP type. Specific toxicological effects were inferred from the functions of genes whose transcripts either increased or decreased. Data analysis resulted in important differences and also similarities among the NPs. Elevated levels of transcripts of several marker genes for stress were observed, suggesting that only nZnO caused nonspecific global stress to the cells under environmentally relevant conditions. Genes with photosynthesis-related functions were decreased drastically during exposure to nTiO 2 and slightly during exposures to the other NP types. This pattern suggests either toxicological effects in the chloroplast or effects that mimic a transition from low to high light. nAg exposure dramatically elevated the levels of transcripts encoding known or predicted components of the cell wall and the flagella, suggesting that it damages structures exposed to the external milieu. Exposures to nTiO 2 , nZnO, and QDs elevated the levels of transcripts encoding subunits of the proteasome, suggesting proteasome inhibition, a phenomenon believed to underlie the development and progression of several major diseases, including Alzheimer's disease, and used in chemotherapy against multiple myeloma. N anoparticles (NPs) are materials with at least one dimension in the nanoscale (ca. 1 to 100 nm), with resulting size-related physicochemical properties that differ from those of their bulk counterparts. Specifically, their high surface-to-volume ratio generally results in highly reactive and physicochemically dynamic materials. With the increasing use of engineered NPs, there is an enormous uncertainty with respect to their potential environmental impacts, including their toxicological effects. Of special concern is the emerging evidence that numerous NPs can produce reactive oxygen species (ROS), release toxic metals, or react directly with the biological membrane (1, 2). A major key question is whether toxicological effects are due to general properties shared by diverse NP types or whether they are specific to each NP. For example, if toxicological effects are related to the size, shape, or agglomeration of the NP, then different NP compositions would likely exert similar effects (3), whereas distinct toxicological effects would be expected if particle composition controls the interaction with the biological surface (4, 5). An understanding of the toxicological effects of each NP type is critical for any prediction of their immediate and long-term risks for humans and ecosystems.A powerful approach to determine how an organism responds to a particular abiotic condition...

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mentioning

confidence: 99%

Transcriptome Sequencing (RNA-seq) Analysis of the Effects of Metal Nanoparticle Exposure on the Transcriptome of Chlamydomonas reinhardtii

Simon

Domingos

Hauser

et al. 2013

Appl Environ Microbiol

117

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“…Other similarities with Tetraselmis sp. were observed in C. reinhardtii, i.e., as the concentration of TiO 2 -Np increased, the number of damaged cells also increased [27]. Most microalgae have negatively charged cell surfaces, caused by the ionization of ionogenic functional groups on the cell walls and also by the adsorption of ions from the culture medium.…”

Section: Nanoecotoxicological Effects Of a Sunscreen Formulation Basementioning

confidence: 88%

“…Moreover, TiO 2 -Np may reduce the availability of light to algae entrapped clusters, inhibiting their growth [4]. The algae Chlamydomonas reinhardtii [27] and Dunaliella tertiolecta [18] also showed TiO 2 -Np aggregates on their cell surface. If this effect also occurs in Tetraselmis sp., we suggest that the occurrence of Np aggregates on algal surfaces may be one of the reasons for the cell precipitation observed in this study.…”

Section: Resultsmentioning

confidence: 99%

Nanoecotoxicological Effects of a Sunscreen Formulation based on Tio2 Nanoparticles on Microalgae from Guanabara Bay (Rio de Janeiro, Brazil)

Abe¹,

Cunha²,

Salomon³

et al. 2017

MOJPS

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The rise of nanotechnology has resulted in an increase in the use of nanoproducts, such as sunscreens based on nanoparticles of TiO 2 (TiO 2 -Np), which reach aquatic ecosystems through their manufacture, consumption and disposal. Studies indicate that TiO 2 -Np have an ecotoxicological potential, inactivating algae from the Anabaena, Microcystis and Melosira genera. Tetraselmis sp., abundant phytoplanktonic organisms of Guanabara Bay (Rio de Janeiro, Brazil), has been exposed to any discarded material released into this environment. In order to evaluate the effects of TiO 2 -Np on Tetraselmis sp., treatments with different doses of TiO 2 -Np were performed and their morphology, morphometry, surface charge, chlorophyll a rate and catalase production were analyzed. Under normal culture conditions, doses over 25 mg.L -1 caused intense precipitation, agglomeration and disruption of the cells, with remarkable variation of the zeta potential. No significant modification in the amount of chlorophyll a was observed, while the catalase production decreased only by 100 mg.L -1 of TiO 2 -Np. The results showed that TiO 2 -Np induced several effects in algae, highlighting their cell agglomeration. In conclusion, TiO 2 -Np provoked harmful effects in Tetraselmis sp., an important phytoplanktonic organism of Guanabara Bay (Rio de Janeiro, Brazil), and may disturb the dynamics of this ecosystem.

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“…In reflectance mode, CLSM has convincingly detected the internalisation of unlabelled Ag ENPs and CeO 2 ENPs by cultured fish and human cells (Gaiser et al 2012). Further, CLSM has also been used to demonstrate the attachment of TiO 2 ENPs to micro-algal cell surfaces (Chen et al 2012a) and the transport of quantum dots across the gut epithelium of exposed Daphnia magna and Ceriodaphnia dubia (Feswick et al 2013). Detailed imaging using CLSM of resin sections of exposed cladocerans has further shown that ENPs are predominantly located inside the digestive tract, but not within the gut epithelia or the brood chamber of the exposed organisms (Li et al 2011).…”

Section: Light Confocal Dark Field and Spectroscopy Detection And Amentioning

confidence: 95%

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms

et al. 2014

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Initiatives to support the sustainable development of the nanotechnology sector have led to rapid growth in research on the environmental fate, hazards and risk of engineered nanoparticles (ENP). As the field has matured over the last 10 years, a detailed picture of the best methods to track potential forms of exposure, their uptake routes and best methods to identify and track internal fate and distributions following assimilation into organisms has begun to emerge. Here we summarise the current state of the field, focussing particularly on metal and metal oxide ENPs. Studies to date have shown that ENPs undergo a range of physical and chemical transformations in the environment to the extent that exposures to pristine well dispersed materials will occur only rarely in nature. Methods to track assimilation and internal distributions must, therefore, be capable of detecting these modified forms. The uptake mechanisms involved in ENP assimilation may include a range of trans-cellular trafficking and distribution pathways, which can be followed by passage to intracellular compartments. To trace toxicokinetics and distributions, analytical and imaging approaches are available to determine rates, states and forms. When used hierarchically, these tools can map ENP distributions to specific target organs, cell types and organelles, such as endosomes, caveolae and lysosomes and assess speciation states. The first decade of ENP ecotoxicology research, thus, points to an emerging paradigm where exposure is to transformed materials transported into tissues and cells via passive and active pathways within which they can be assimilated and therein identified using a tiered analytical and imaging approach.

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Toxicological effects of nanometer titanium dioxide (nano-TiO2) on Chlamydomonas reinhardtii

Cited by 163 publications

References 47 publications

Transcriptome Sequencing (RNA-seq) Analysis of the Effects of Metal Nanoparticle Exposure on the Transcriptome of Chlamydomonas reinhardtii

Transcriptome Sequencing (RNA-seq) Analysis of the Effects of Metal Nanoparticle Exposure on the Transcriptome of Chlamydomonas reinhardtii

Nanoecotoxicological Effects of a Sunscreen Formulation based on Tio2 Nanoparticles on Microalgae from Guanabara Bay (Rio de Janeiro, Brazil)

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms

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