Toxicological effects of trichlorfon on hematological and biochemical parameters in Cyprinus carpio L. following thermal stress

Woo, Soo Ji; Kim, Na Young; Kim, Shin Hu; Ahn, Sang Jung; Seo, Jung Soo; Jung, Sung Hee; Cho, Mi Young; Chung, Joon Ki

doi:10.1016/j.cbpc.2018.03.001

Cited by 24 publications

(10 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The pollution in the aquatic fauna can be observed in the fishes as they are the best indicators. Hence their haematological parameters can be used for the toxicity evaluation (Sharma and Singh, 2004 andRamesh et al, 2014;Woo et al, 2018;Bojarski and Witeska, 2020). In the present haematological study, there was a significant decrease in the RBC counts, TLC counts, Differential counts, Haemoglobin percentage, PCV percentage, Eosinophils and Monocytes percentage after fenvalerate exposure while after neem extract treatment there was a significant decrease in their levels.…”

Section: Resultssupporting

confidence: 49%

Restorative effect of Azadirachta indica against fenvalerate induced haematological and biochemical toxicity in a freshwater fish Clarias batrachus

Kumari

Jigyasu

Paul

2020

JANS

View full text Add to dashboard Cite

Pesticide use in recent times has increased many folds to enhance the yield of crops. The water bodies like ponds and others are extensively used for the cultivation of fishes. Since these fishes are a good source of protein and nutritious value, hence are consumed by humans. These pesticide-contaminated fishes are consumed by the human, causing health hazards. The objective of the present research work was to know the bio remedial effect of Azadirachta indica on fenvalerate induced toxicity in a freshwater air-breathing catfish "Mangur" known as Clarias batrachus ( Linnaeus). The fishes were treated with 1/3rd of LC50, i.e. 0.92 ppm of a pyrethroid pesticide fenvalerate and observed the fishes for 96 hours. Thereafter, the aqueous leaf extract of Azadirachta indica was administered orally, daily by gastric intubation method at the dose of 100 mg/Kg body weight per day for 15 days. The study revealed that, after the exposure of 0.92 ppm of fenvalerate, there was a significant decrease in RBC count from 2.76 to 2.14 X (106) (mm-3), Total leukocyte count from18.10 to 16.30 X (103) (mm-3), , packed ell volume from 24 % to 16% , haemoglobin from 6.89 g/100 ml to 4.23 g/100ml of blood whereas increase in the level of serum glucose and cholesterol level from 50.33 mg/dl to 103.73 mg/dl and 168.52 g/dl to 248.99 g/dl respectively. At the same time, serum protein level decreased from 3.16g/dl to2.52 g/dl due to pesticide in the experimental fishes. But, after the administration of aqueous leaf extract of Azadirachta indica there was significant normalisation in the test parameters of the fishes. The study indicated that neem leaves possessed restorative effect against fenvalerate induced toxicity in Clarias batrachus.

show abstract

Section: Resultssupporting

confidence: 49%

Restorative effect of Azadirachta indica against fenvalerate induced haematological and biochemical toxicity in a freshwater fish Clarias batrachus

Kumari

Jigyasu

Paul

2020

JANS

View full text Add to dashboard Cite

show abstract

“…Additionally, in another study, chlorpyrifos inhibited CYP 1A, 2B and 3A after intraperitoneal injection to rats at a dose of 50 mg/kg [26] and in case of CYP2B6, the heme loss was the major mechanism leading to the inactivation of CYP2B6 by chlorpyrifos [9]. Furthermore, trichlorfon was demonstrated to cause a significant rise in the mRNA expression of CYP1A in the liver tissues indicating its cytotoxic effect on hepatocytes due to the destruction of the structure and integrity of cell organelles, such as the endoplasmic reticulum, and membrane transport systems [29].…”

Section: Discussionmentioning

confidence: 99%

Effects of several organophosphates on hepatic cytochrome P450 activities in rats

Abdou

Elbadawy

Khalil

et al. 2020

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Four commonly used organophosphates (fenitrothion, dichlorvos, chlorpyrifos, and trichlorfon) were orally administered to male Sprague-Dawley rats for five days in order to explore their effects on the activities of liver cytochrome P450 (CYP). In addition, Michaelis-Menten kinetics of the metabolic reactions catalyzed by liver CYPs were analyzed following the addition of these compounds to the assay system to examine their potential inhibitory effects on liver CYPs activities. These reactions included ethoxyresorufin O-deethylation, midazolam 4-hydroxylation, tolbutamide hydroxylation, and bufuralol 1'-hydroxylation for CYP1A, 3A, 2C, and 2D activities, respectively. Total CYP content was also examined after oral administration of each organophosphate. Results revealed that oral giving of fenitrothion inhibited significantly CYP1A and 3A activities while elevated activity of CYP2C. Fenitrothion is a potent inhibitor for CYP1A and 2C with Ki values of 0.42 and 36.1 μM, respectively but had a weak inhibitory effect on CYP2D and 3A with Ki values of 290 and 226 μM, respectively. Chlorpyrifos is a potent inhibitor of CYP1A with Ki 0.24 μM and moderately inhibited CYP2C or 3A with Ki values of 84.8 and 77.7 μM, respectively. On the other hand, dichlorvos and trichlorfon caused extremely low or negligible inhibition of different CYP activities. From these results, it is concluded that both fenitrothion and chlorpyrifos may increase the toxicity of chemicals in environmental living organisms through their potent inhibitory effects on these CYP activities, but dichlorvos and trichlorfon may not.

show abstract

“…Pharmaceuticals may alter the transaminase and other enzymes in the tissues, which are responsible for cellular processes. Hence, enzyme assays are widely used in most of the pharmaceutical toxicity assessment (Woo et al., 2018). Moreover, the health status of the vital organs, such as gills, liver, and kidney could be monitored using the enzyme activities.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, high level of transaminases could improve the synthesis/disturbance in cell integrity, by which the enzyme concentrations in the cells do not allow for distribution, this could increase the internal enzyme concentration (Yang et al., 2013). Increase in GPT and GOT activity in the organs of fish exposed to toxicants indicate that the fish developed alleviating mechanism to compensate the impaired metabolism (Woo et al., 2018). An elevation in GPT activity in various organs (gill, liver and muscle) was also noted in Cyprinus carpio exposed to pharmaceutical drug carbamazepine (Malarvizhi et al., 2012).…”

Section: Discussionmentioning

confidence: 99%

Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities

Umamaheswari

Renuka

Ramesh

et al. 2019

Heliyon

View full text Add to dashboard Cite

Labeo rohita were exposed to amoxicillin at a concentration of 1 mg/L (Treatment -I) and 0.5 mg/L (Treatment-II) for a period of 35 days. Numerous alterations were found in amoxicillin treatment groups when compared to the control group. Hemoglobin (Hb), hematocrit (Hct), and erythrocytes (RBCs) levels were significantly ( P < 0.05) decreased. Leukocytes (WBC), mean cell volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) levels were significantly ( P < 0.05) increased. In the plasma and gill tissues, ionic compounds (sodium, potassium, and chloride) levels were significantly declined throughout the treatment period. The plasma biochemical profiles were significantly altered: glucose level remained low (except at the end of 7 th day in Treatment -I) till 35 days of the treatment period. Biphasic trend occurred in the protein level, significant increase was observed on 7 th and 28 th day (Treatment -I and -II), and 35 th day (Treatment -I), and in remaining days its level was found to be decreased. Glutamate oxaloacetate transaminase (GOT) activity in the plasma was inhibited significantly, whereas in the gill, liver, and kidney tissues the enzyme activity was elevated. Plasma glutamate pyruvate transaminase (GPT) activity was inhibited throughout the study period. GPT activity in the gill was found to be elevated during the treatment period. Liver GPT activity was elevated in all the treatments except 28 th (Treatment-I) and 35 th day (Treatment-I, and II). GPT activity in the kidney was elevated (except 14 th day in Treatment-II). Lactate dehydrogenase (LDH) activity was inhibited in plasma (except 14 th day in Treatment-II), gill, liver (except 7 th day in Treatment-I), and kidney tissues significantly ( P < 0.05). The present study emphasizes that amoxicillin at 1 and 0.5 mg/L concentrations affects the hematological/biochemical/electrolytes/enzymological parameters of fish and these biomarkers serve as an effective test system for environmental risk assessment of pharmaceuticals in the aquatic environment.

show abstract

Toxicological effects of trichlorfon on hematological and biochemical parameters in Cyprinus carpio L. following thermal stress

Cited by 24 publications

References 58 publications

Restorative effect of Azadirachta indica against fenvalerate induced haematological and biochemical toxicity in a freshwater fish Clarias batrachus

Restorative effect of Azadirachta indica against fenvalerate induced haematological and biochemical toxicity in a freshwater fish Clarias batrachus

Effects of several organophosphates on hepatic cytochrome P450 activities in rats

Chronic amoxicillin exposure affects Labeo rohita: assessment of hematological, ionic compounds, biochemical, and enzymological activities

Contact Info

Product

Resources

About