Toxicological evaluation of a standardized hydroethanolic extract from leaves of Plantago australis and its major compound, verbascoside

Henn, Jeferson Gustavo; Steffens, Luiza; Sperotto, Nathalia Denise de Moura; Ponce, Betânia de Souza; Veríssimo, Rodrigo Moisés; Boaretto, Fernanda Brião Menezes; Hassemer, Gustavo; Péres, Valéria Flores; Schirmer, Helena; Picada, Jaqueline Nascimento; Saffi, Jenifer; Moura, Dinara Jaqueline

doi:10.1016/j.jep.2018.10.003

Cited by 24 publications

(19 citation statements)

References 38 publications

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“…Furthermore, Perucatti et al, have reported that in vivo cytogenetic tests that is feeding 5 mg/Kg acteoside to rabbit (Oryctolagus cuniculus) for 80 days revealed no toxicity with any other mutagenic activity, resulting in no cytotoxic for the animals [24]. These studies suggest that acteoside is a bioactive material that can be used in both animal and human diets [6,24]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 93%

“…Acteoside (called as verbascoside; C 29 H 36 O 15 ) is a glycoside that is isolated from the owers or leaves of many herbal plants such as Scrophularia ningpoensis, Cistanche deserticola, Digitalis purpurea, and Osmanthus fragrans [22,23]. Recently, Henn et al, reported that the high concentration (100 µg/mL) of acteoside isolated from the leaves of Plantago australis did not only show a less cytotoxicity in V79 Chinese hamster cells used as a normal cells but also did not have mutagenic or genotoxic activities and phototoxic properties [6]. Furthermore, Perucatti et al, have reported that in vivo cytogenetic tests that is feeding 5 mg/Kg acteoside to rabbit (Oryctolagus cuniculus) for 80 days revealed no toxicity with any other mutagenic activity, resulting in no cytotoxic for the animals [24].…”

Section: Discussionmentioning

confidence: 99%

“…As shown in Fig. 1 [4], Buddleja globose [5], and Plantago australis [6]. Acteoside has various biological activities such as antimicrobial [5], antiin ammatory, [7] anticancer [8], antioxidative [9], cytoprotective [9], and neuroprotective effect [10].…”

Section: Introductionmentioning

confidence: 99%

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Acteoside counteracts interleukin-1β-induced catabolic processes through the modulation of mitogen-activated protein kinases and the NFκB cellular signaling pathway

Lim

Kim

et al. 2020

Preprint

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Objectives To verify the anti-catabolic effects of acteoside [α-L-rhamnosyl-(1->3)-β-D-glucoside] against osteoarthritis and its anti-catabolic signaling pathway. Methods Primary rat chondrocytes were isolated enzymatically from the articular cartilage of rat knee joint. Cytotoxicity of acteoside was assessed by MTT and Cell Live/Dead assay. Proteoglycan content was measured by dimethylmethylene blue assay. The proteoglycan loss was assessed by histological analysis using safranin-O & fast green staining after ex vivo organ culture of articular cartilage. The alteration of catabolic factors such as cartilage degrading enzymes, pro-inflammation cytokines, and inflammatory mediators were assessed by qPCR, qRT-PCR, gelatin zymography, western blot, and cytokine array. Cellular signaling pathways were investigated by western blot and nucleus translocation. Acteoside was orally administrated to osteoarthritic animals generated by the destabilization of medial meniscus at the knee joint of mice for 8 weeks. Thereafter, proteoglycan loss was assessed by safranin-O & fast green staining. Results Acteoside did not decrease the viabilities of mouse fibroblast L929 cells used as a normal cells and primary rat chondrocytes. Acteoside counteracted the IL-1β-induced proteoglycan loss in the chondrocytes and articular cartilage through suppressing the expression and activation of cartilage-degrading enzyme such as matrix metalloproteinase (MMP)-13, MMP-1, and MMP-3. Furthermore, acteoside suppressed the expression of inflammatory mediators such as inducible nitric oxide synthase, cyclooxygenase-2, nitric oxide, and prostaglandin E2 in the primary rat chondrocytes treated with IL-1β. Subsequently, the expression of pro-inflammatory cytokines was decreased by acteoside in the primary rat chondrocytes treated with IL-1β. Moreover, acteoside suppressed not only the phosphorylation of mitogen-activated protein kinases in primary rat chondrocytes treated with IL-1β but also the translocation of NFκB from the cytosol to the nucleus through suppression of its phosphorylation. Oral administration of 5 and 10 mg/kg acteoside attenuated the progressive degeneration of articular cartilage in the osteoarthritic mouse model generated by destabilization of the medial meniscus. Conclusion Our findings indicate that acteoside is a promising potential anti-catabolic agent or supplement to attenuate or prevent progressive degeneration of articular cartilage.

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Acteoside counteracts interleukin-1β-induced catabolic processes through the modulation of mitogen-activated protein kinases and the NFκB cellular signaling pathway

Lim

Kim

et al. 2020

Preprint

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“…Furthermore, conflicting results about the mutagenic potential of verbascoside, a phenylethanoid compound, identified in F. angustifolia Vahl. when tested on TA 98 and TA 100, did not induce frameshift and base-pair substitution mutations in the presence or absence of metabolic system [46], but induced DNA damages on human lymphocytes, with an involvement of PARP-1 and p53 expression through its caffeic acid moiety [47].…”

Section: Plos Onementioning

confidence: 95%

Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition

et al. 2020

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In recent years, chronic degenerative diseases such as certain types of cancers, are becoming an evident issue. DNA damage has been for long recognized as a causal factor for cancer development because mutations or chromosomal aberrations affect oncogenes and tumor suppressor genes leading cells to malignant transformation and to the subsequent cancerous growth. Medicinal plants are often used for the prevention or treatment of various diseases with great scientific interest. Among the medicinal plants distributed in the Mediterranean region, Fraxinus angustifolia Vahl. has been used in traditional medicine for its remarkable curative properties. However, in spite of this popularity, little works have been performed on the activity so that further studies should be performed to investigate in depth the antimutagenic, antigenotoxic and antiproliferative activities of the plant. Thus, the present study was aimed to the evaluation of the potential antimutagenic, antigenotoxic and antiproliferative properties of leaves and stem bark extracts of this well-known tree. Antimutagenic activity was evaluated by Salmonella mutagenicity assay in Salmonella typhimurium TA98 and TA100 strains. The antigenotoxic potential was assessed by umu test in the strain of S. typhimurium TA1535/pSK1002. Antiproliferative activity was studied on human hepatoblastoma (HepG-2) and on breast adenocarcinoma (MCF-7) cell lines by MTT assay. Furthermore, the antiproliferative activity observed on cancer cells was compared with that on the human normal-like fibroblasts (TelCOFS02MA) and the selectivity index was calculated to understand if extracts were able to exert selective toxicity towards cancer cells. Moreover, phenolic compounds are plant substances with a large spectrum of biochemical activities with antioxidant, antimutagenic and anticarcinogenic effects. Based on the strong evidence of biological activities of phenolic compounds, the study was focused on the determination of total phenolics and flavonoids contents, and the phytochemical composition of the extracts assessed by LC/MS. The ethanol extracts of both leaves and stem barks showed

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“…Подорожник является одним из 200 наиболее хорошо охарактеризованных и описанных видов семейства Plantaginaceae и официнальным фармацевтическим средством в Европейской фармакопее [16]. Основные активные фармацевтические ингредиенты листьев подорожника (каталпол, аукубин и актеосайд) относятся к группе фенилэтаноидных и иридоидных гликозидов, обеспечивают противовоспалительный [12], антиоксидантный [13,36], иммуномодулирующий [18], а также ряд других эффектов [20,29].…”

Section: характеристика растительных препаратов эвкабал сироп и эвкабunclassified

Новые Терапевтические Возможности Фитопрепаратов При Лечении Острых Респираторных Инфекций У Детей

Агафонова¹,

Никулина²,

Петренко³

et al. 2021

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1 ГУ «Днепропетровская медицинская академия МЗ Украины», г. Днепр, Украина 2 КУ «Днепровская городская детская клиническая больница № 5», г. Днепр, Украина Новые терапевтические возможности фитопрепаратов при лечении острых респираторных инфекций у детей Резюме. Поскольку респираторные вирусные инфекции являются самоэлиминирующимися заболеваниями, во всех случаях неосложненного течения острых респираторных инфекций мы считаем наиболее обоснованной симптоматическую терапию. Важной частью этой терапии у детей являются фитопрепараты, при условии применения только тех растительных лекарственных средств, которые обладают высоким профилем эффективности и безопасности. В статье представлены новые данные о поливалентных (противовирусных, противомикробных, иммуностимулирующих и противокашлевых) свойствах растительных препаратов ЭВКАБАЛ ® (Esparma GmbH, Германия) -Эвкабал СИРОП и Эвкабал БАЛЬЗАМ, предназначенных для лечения острых респираторных инфекций у детей. Ключевые слова: фитопрепараты; острые респираторные инфекции; кашель; дети; симптоматическая терапия

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Toxicological evaluation of a standardized hydroethanolic extract from leaves of Plantago australis and its major compound, verbascoside

Cited by 24 publications

References 38 publications

Acteoside counteracts interleukin-1β-induced catabolic processes through the modulation of mitogen-activated protein kinases and the NFκB cellular signaling pathway

Acteoside counteracts interleukin-1β-induced catabolic processes through the modulation of mitogen-activated protein kinases and the NFκB cellular signaling pathway

Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition

Новые Терапевтические Возможности Фитопрепаратов При Лечении Острых Респираторных Инфекций У Детей

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