Toxicological evaluation of Pterocaulon polystachyum extract: A medicinal plant with antifungal activity

Regner, Gabriela Gregory; Gianesini, Janaína; Borowski, Rafael Gomes Von; Silveira, Fabiana; Semedo, Juliane Garcia; Ferraz, Alexandre de Barros Falcão; Wiilland, Elenir de Fátima; Poser, Gilsane von; Allgayer, Mariângela da Costa; Picada, Jaqueline Nascimento; Pereira, Patrı́cia

doi:10.1016/j.etap.2010.11.003

Cited by 20 publications

(7 citation statements)

References 21 publications

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“…Contrary to allopathy, the traditional usage of medicinal plants is apparently considered to be safe and hence the toxicity of traditional herbal medicines has not been totally evaluated in most cases, although medicinal plants can be extremely harmful to human health. Studies have revealed that some plants frequently used in folk medicine are potentially genotoxic [ 30 – 32 ]. It is thus truly relevant to screen the genotoxicity during the preclinical evaluation of herbal extracts or substances, in order to verify their mutagenic potential for both safety and economic concerns, since plants are widely used in folk medicine and can be a resource for the development of new drugs [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant Activity and Genotoxic Assessment of Crabwood (Andiroba, Carapa guianensis Aublet) Seed Oils

Araújo-Lima

Fernandes

Gomes

et al. 2018

Oxidative Medicine and Cellular Longevity

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The seed oil of Carapa guianensis (Aublet), a tree from the Meliaceae family commonly known as andiroba, is widely used in Brazilian traditional medicine because of its multiple curative properties against fever and rheumatism and as an anti-inflammatory agent, antibacterial agent, and insect repellant. Since there is no consensus on the best way to obtain the C. guianensis oil and due to its ethnomedicinal properties, the aim of the present research was to evaluate the chemical composition, free-radical scavenging activity, and mutagenic and genotoxicity properties of three C. guianensis oils obtained by different extraction methods. The phenolic contents were evaluated by spectrophotometry. Oil 1 was obtained by pressing the dried seeds at room temperature; oil 2 was obtained by autoclaving, drying, and pressing; oil 3 was obtained by Soxhlet extraction at 30–60°C using petroleum ether. The oil from each process presented differential yields, physicochemical properties, and phenolic contents. Oil 1 showed a higher scavenging activity against the DPPH radical when compared to oils 2 and 3, suggesting a significant antioxidant activity. All oils were shown to be cytotoxic to bacteria and to CHO-K1 and RAW264.7 cells. At noncytotoxic concentrations, oil 2 presented mutagenicity to Salmonella enterica serovar Typhimurium and induced micronuclei in both cell types. Under the same conditions, oil 3 also induced micronucleus formation. However, the present data demonstrated that oil 1, extracted without using high temperatures, was the safest for use as compared to the other two oils, not showing mutagenicity or micronucleus induction.

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Section: Discussionmentioning

confidence: 99%

Antioxidant Activity and Genotoxic Assessment of Crabwood (Andiroba, Carapa guianensis Aublet) Seed Oils

Araújo-Lima

Fernandes

Gomes

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Moreover, the administration of the essential oil of Psidium cattleianum at doses of 100, 200, and 500 mg.•kg −1 did not cause alterations in body weight or death of any animal (Castro et al, 2015). On the other hand, the acute and subacute oral treatment of a hexane extract of Pterocaulon polystachyum was relatively toxic to the liver and kidney (Regner et al, 2011).…”

Section: Toxicologymentioning

confidence: 88%

Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds

Scorzoni

Sangalli-Leite

Singulani

et al. 2016

Journal of Microbiological Methods

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In the last decades, the increased number of immunocompromised patients has led to the emergence of many forms of fungal infections. Furthermore, there are a restricted arsenal of antifungals available and an increase in the development of resistance to antifungal drugs. Because of these disadvantages, the search for new antifungal agents in natural sources has increased. The development of these new antifungal drugs involves various steps and methodologies. The evaluation of the in vitro antifungal activity and cytotoxicity are the first steps in the screening. There is also the possibility of antifungal combinations to improve the therapy and reduce toxicity. Despite that, the application of the new antifungal candidate could be used in association with photodynamic therapy or using nanotechnology as an ally. In vivo tests can be performed to evaluate the efficacy and toxicity using conventional and alternative animal models. In this work, we review the methods available for the evaluation of the antifungal activity and safety of natural products, as well as the recent advances of new technology in the application of natural products for antifungal therapy.

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“…They were treated for 28 days with single doses (0.1 mL/10 g body‐weight) of GFC (2, 10 or 20 mg/kg), saline, Tween 5% or imipramine (20 mg/kg). The animals were weighed and observed daily for physiological and behavioural changes .…”

Section: Methodsmentioning

confidence: 99%

A 28‐day Sub‐acute Genotoxic and Behavioural Assessment of Garcinielliptone FC

Coelho

Prado

Rossato

et al. 2018

Basic Clin Pharma Tox

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Garcinielliptone FC (GFC) is a polyisoprenylated benzophenone isolated from Platonia insignis Mart (Clusiaceae) with promising anticonvulsant properties. However, its safe use and other effects on the central nervous system require assessment. This study assessed the toxicological effects of GFC using the comet assay and the micronucleus test in mice treated for 28 days. A behavioural model was employed to detect possible injuries on the central nervous system. Mice treated with GFC (2, 10 and 20 mg/kg; i.p.) daily for 28 days were submitted to rotarod test, open-field test and tail suspension test (TST). After the behaviour tasks, biological samples were assessed to evaluate genotoxic and mutagenic effects using the comet assay and the micronucleus test. Garcinielliptone FC did not impair the performance of the animals in the rotarod and open-field tests, with no antidepressant-like effect in TST. No genotoxic effects in blood and cerebral cortex were observable in the comet assay; however, there was a significant increase in index and frequency of damage in liver after treatment with GFC 20 mg/kg. Garcinielliptone FC did not increase micronucleus frequency in bone marrow. At the tested doses, GFC was not toxic to the CNS and did not induce genotoxic damage to blood or bone narrow cells. DNA damage to liver tissue was caused only by the highest dose, although no mutagenic potential was observed.

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Toxicological evaluation of Pterocaulon polystachyum extract: A medicinal plant with antifungal activity

Cited by 20 publications

References 21 publications

Antioxidant Activity and Genotoxic Assessment of Crabwood (Andiroba, Carapa guianensis Aublet) Seed Oils

Antioxidant Activity and Genotoxic Assessment of Crabwood (Andiroba, Carapa guianensis Aublet) Seed Oils

Searching new antifungals: The use of in vitro and in vivo methods for evaluation of natural compounds

A 28‐day Sub‐acute Genotoxic and Behavioural Assessment of Garcinielliptone FC

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