Toxicological evaluation of the ultrasonic extract from Dichroae radix in mice and wistar rats

Abstract

This study was aimed at evaluating the acute and subchronic toxicity of ultrasonic extract of Dichroae radix (UEDR) in mice and rats. High performance liquid chromatography (HPLC) and thin layer chromatogrephy (TLC) were used to detect β-dichroine and α-dichroine in UEDR for quality control. The levels of β-dichroine and α-dichroine in UEDR were 1.46 and 1.53 mg/g, respectively. An oral LD50 of 2.43 g/kg BW was observed in acute toxicity test. After 28-day repeated oral administration, compared with the control group, treatment-related changes in body weight (BW) and body weight gain (BWG), lymphocyte counts and ratios, as well as in the relative organ weights (ROWs) of liver, kidney, lung, and heart, were detected in the middle- and high-dose groups (P < 0.05, P < 0.01), no differences were noted in the serum biochemical parameters and necropsy examinations in both sexes at all doses. Histopathological examinations exhibited UEDR-associated signs of toxicity or abnormalities. After 14 days withdrawal, no statistically significant or toxicologically relevant differences were observed in any of the UEDR-treated groups, and the hispathological lesions in the high-dose group were alleviated. Findings showed that long-course and high-dose of UEDR administration was toxic, and showed dose-dependence, the toxic damage was reversible.

Phytochemical Composition and Anti-microbial Properties of Petersianthus macrocarpus (P.Beauv.) Liben (Lecythidaceae) Stem Bark Extract and Fractions

“…Chronic effects are more related to the level of a substance accumulated in the organism or even in specific tissue after many months or even years of repeated ingestion. 25 The results obtained from toxicity tests showed that the acute toxicity of CEE is low, but chronic administration may result in deleterious effects. Compared with the control, significant decrease (p<0.05) in body weight was observed in the group treated with 600 mg/kg body weight of CEE.…”

Acute and 28-Day Repeated-Dose Oral Toxicity of the Herbal Formula Guixiong Yimu San in Mice and Sprague–Dawley Rats

“…Furthermore, the BW and ROW of the rats' even at the high dose of GYS were not considerably different from those in the control group. Generally, reductions in BWG and ROW are mostly monitored markers of toxicity after contact with chemicals and drugs [34,50]. These outcomes propose that the longtime use of GYS with a high dose of 30.0 g/kg BW did not cause weight loss and delayed growth in the rats.…”

“…All of the experimental animals were monitored for behavioral activities, deaths, and signs and symptoms of gross toxicity twice a day through the 28-day subacute toxicity trial. At the end of the drug administration period (24 h after the last administration), all surviving rats from each group were euthanized for blood hematology, serum biochemistry, histopathology, and relative organ weight (ROW) [33,34].…”