Toxicological Evaluation of Waste-Water Samples to Appropriately Sensitized Cultured Fathead Minnow Cells Compared with the Microtox Assay

Scheers, Ellen; Wielen, Claire Van der; Dierickx, Paul J.

doi:10.1007/s001280246

Cited by 2 publications

(1 citation statement)

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“…According to equation 3, the luminescent bacteria indicated an EC50 of 10.72% and an acute toxicity index of 9.3 ATU, which classified the effluent as very toxic. In studies conducted by Rosa et al (2001), Scheers et al (2002), Somensi et al (2010) and Bedoui et al (2015), the luminescent bacteria, widely used in ecotoxicological experiments, also indicated high levels of toxicity of textile effluents, with EC50 values of 5.4, 13.0, 3.5 and 3.0% respectably.…”

Section: Ecotoxicological Parametersmentioning

confidence: 98%

Microbial Population Inhibition Method Through Spectrophotometry Absorption of Visible Light Applied to Ecotoxicological Analyses

Mainardi

Bidóia

2020

JAB

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Ecotoxicology is a science that studies the effects of pollutants and forecast their transformations on the environment. Ecotoxicological studies have been used in soil and water quality assessment, development and implementation of new techniques of water and effluent treatment, tools for better industrial management, bioremediation techniques and sustainable agriculture approaches. Microorganisms, which were seen to detain a fundamental importance in nutrient cycling and energy flow, have been increasingly used as bioindicators in ecotoxicological analyses. The populational inhibition of microbiological strains may be measured through the absorbance of visible light, an efficient, fast, low-cost and reliable method that has been widely used in qualitative and quantitative analysis. In this manuscript, a real textile effluent sample was analyzed regarding its electric conductivity, pH, turbidity, solids, alkalinity, biochemical oxygen demand (BOD5), chemical oxygen demand (COD) and a spectrophotometry microbial population inhibition (MPI) method using the Bacillus subtilis bacteria and the Saccharomyces cerevisiae yeast. The EC20, EC50 and acute toxicity indexes were satisfactory in relation to the widely used method of light reduction of the Vibrio fischeri luminescence bacteria. The MPI was shown to be a feasible method to determine the hazardous effects caused by the textile effluent sample towards the microbial populations.

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Section: Ecotoxicological Parametersmentioning

confidence: 98%

Microbial Population Inhibition Method Through Spectrophotometry Absorption of Visible Light Applied to Ecotoxicological Analyses

Mainardi

Bidóia

2020

JAB

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Application of the Recombinant Bioluminescence Bacterium on the Toxicity Assessment of the Sole Chemicals and Soil Samples

Kong¹,

Kim²,

Lee³

et al. 2012

Journal of Korean Society of Environmental Engineers

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Various factors affecting on the bioassay were investigated. Experiments with a low mixture ratio (cell to toxicant solution) of 0.5 : 9.5 (v/v) produced observable bioluminescence intensity for assay. Both sodium lactate and potassium nitrate stimulate bioluminescence activity; 2.6~4.0 times of control. Distilled water and MSM, which gave non significant effects on the bioluminescence activity, were determined as proper diluent or extract solutions. A wide range of toxic responses of metals and organics were observed. In general, organics were much less sensitive than metals. Samples collected from eleven sites showed the bioluminescence activity ranging from 29 to 111% of the control. Significant correlation between toxicity and total metal contents was not observed, but the toxicity of two groups, sorted based on the contaminated arsenic concentration in soils, was 44% and 20%, showing considerable differences.

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Toxicological Evaluation of Waste-Water Samples to Appropriately Sensitized Cultured Fathead Minnow Cells Compared with the Microtox Assay

Cited by 2 publications

References 0 publications

Microbial Population Inhibition Method Through Spectrophotometry Absorption of Visible Light Applied to Ecotoxicological Analyses

Microbial Population Inhibition Method Through Spectrophotometry Absorption of Visible Light Applied to Ecotoxicological Analyses

Application of the Recombinant Bioluminescence Bacterium on the Toxicity Assessment of the Sole Chemicals and Soil Samples

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