Toxicological Studies on Laccase from Myceliophthora thermophila Expressed in Aspergillus oryzae

An unusual ring—A opening and other reactions in steroid transformation by the thermophilic fungus Myceliophthora thermophila

“…Comparison of the 1 H and 13 C NMR data of 4-hydroxy-3,4-seco-pregn-20-one-3-oic acid (11) 9.35%). Ultimately the molecular structure was unequivocally defined by X-ray crystallography (Fig.…”

“…This organism is known to generate exogenous enzymes such as laccase [7], xylanases [8,9] and cellulases [10] which, have found practical application in the food industry [11], as biocatalysts [12] in spectroscopic science [13] and biosensor technologies [14]. In order to determine the pattern of metabolic handling of steroids by this fungus, a range of structurally diverse steroidal compounds namely progesterone Transformation products 5␣-Androstan-3␣,17␤-diol (6) 0.73 0.79 3.63 (1H, t, J = 8.6 Hz, 17␣-H), 4.04 (1H, t, J = 2.6 Hz, 3␤-H) 11␤-Hydroxy-progesterone (7) 5.82 0.80 1.38 2.29 4.37 (1H, t, J = 2.5 Hz, 11␣-H) 11␣-Hydroxy-progesterone (8) 5.73 0.70 1.32 2.13 4.03 (1H, td, J = 5 Hz, J = 10 Hz, 11␤-H) 14␣-Hydroxy-androst-4-en-3,17-dione (9) 5.75 1.05 1.22 17␤-Hydroxy-5␣-androstan-3-one (10) 0.76 1.02 3.64 (1H, t, J = 8.6 Hz, 17␣-H) 4-Hydroxy-3,4-seco-pregn-20-one-3-oic acid (11) 0 of sterile media and were incubated for 72 h at 40 • C. The cultures were shaken at 180 rpm on an orbital shaker. Aliquots (5 mL) from the seed flask were transferred aseptically to 10 flasks and grown for a further 72 h as above, at the end of which the fungus is in log phase growth.…”

“…-20-one-3-oic acid(11) C21 H 34 O 5 , Mr: 366.48, orthorhombic space group P2 1 2 1 2 1 (no. 19), a = 9.2910(4), b = 9.3913(3), c = 22.5610(9) Å,˛=ˇ= = 90 • , V = 1968.55(13) Å 3 , Z = 4, D calc = 1.24 Mg/m 3 , u = 0.09 mm −1 , F(000) 800.…”

Wood production, wood technology, and biotechnological impacts

“…Application of laccases can have beneficial effects on the flavour of foodstuff and, in baking, it improves dough and bread characteristics by strengthening the gluten structures (reviewed in . Applications for laccases have been proposed in health care products such as mouthwash, toothpaste, mints, and gums in order to prevent oral malodour (Brinch & Pedersen 2002), and in cosmectics for lightening the skin (Golz-Berner et al 2004) or dyeing human keratin fibres (Josse 2000). In the leather industry, laccases may be used in removal of lignicellulose-containing dung cladding from the animal hides (Auer et al 1999) and, more importantly, in replacing environmentally precarious chrome-based tanning agents in the process of leather making (Thanikaivelan et al 2004(Thanikaivelan et al , 2005 and in turning natural polyphenols in the animal skins or added phenolic compounds into lightfast pigments (Covington et al 2005, Surpano et al 2005.…”

Preserving boiled eggs with a sterilization system employing microbial laccase and wood vinegar

“…This enzyme is now expressed by a strain of Aspergillus oryzae . It is produced by submerged fermentation and recovered by purification and concentration of the fermented culture broth at an industrial scale (Brinch & Pedersen 2002).…”