Toxoplasma gondii

Zygmunt, Deborah J.

doi:10.1086/646152

Cited by 6 publications

(6 citation statements)

References 23 publications

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“…Only one of them, p83/100, occurred as a single spot on the pattern. teria for positive immunoblots: for IgM immunoblots at least two of the following three bands, OspC, 39, 41 [57] and for IgG immunoblots at least five of the ten following bands, 18, OspC, 28,30,39,41,45,58,66, 83/100 [58]. Standardization of immunoblots has not been established so far in Europe.…”

Section: Detection Of Immune-relevant Proteins In Borrelia Gariniimentioning

confidence: 74%

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Proteomics in human disease: Cancer, heart and infectious diseases

et al. 1999

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In recent years, genomics has increased the understanding of many diseases. Proteomics is a rapidly growing research area that encompasses both genetic and environmental factors. The protein composition represents the functional status of a biological compartment. The five approaches presented here resulted in the detection of disease‐associated proteins. Calgranulin B was upregulated in colorectal cancer, and hepatoma‐derived aldose reductase‐like protein was reexpressed in a rat model during hepatocarcinogenesis. In these two investigations, attention was focused on one protein, obviously differing in amount, directly after two‐dimensional electrophoresis (2‐DE). Additional methods, such as enzyme activity measurements and immunohistochemistry, confirmed the disease association of the two candidates resulting from 2‐DE subtractive analysis. The following three investigations take advantage of the holistic potential of the 2‐DE approach. The comparison of 2‐DE patterns from dilated cardiomyopathy patients with those of controls revealed 25 statistically significant intensity differences, from which 12 were identified by amino acid analysis, Edman degradation or matrix‐assisted laser desorption/ionization‐mass spectrometry (MALDI‐MS). A human myocardial 2‐DE database was constructed, containing 3300 protein spots and 150 identified protein species. The number of identified proteins was limited by the capacity of our group, rather than by the principle of feasibility. Another field where proteomics proves to be a valuable tool in identifying proteins of importance for diagnosis is proteome analysis of pathogenic microorganisms such as Borrelia burgdorferi (Lyme disease) and Toxoplasma gondii (toxoplasmosis). Sera from patients with early or late symptoms of Lyme borreliosis contained antibodies of various classes against about 80 antigens each, containing the already described antigens OspA, B and C, flagellin, p83/100, and p39. Similarly, antibody reactivity to seven different marker antigens of T. gondii allowed differentiation between acute and latent toxoplasmosis, an important diagnostic tool in both pregnancy and immunosuppressed patients.

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Section: Detection Of Immune-relevant Proteins In Borrelia Gariniimentioning

confidence: 74%

“…Up to 90% of primary infections in pregnant women are not recognized [66]. At present, detection is exclusively based on serological screening and PCR.…”

Section: Detection Of Antigens In Toxoplasma Gondiimentioning

confidence: 99%

Proteomics in human disease: Cancer, heart and infectious diseases

et al. 1999

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“…In case of IgA the reaction was more frequent in groups 1 and 2 as expected. Antibodies present in the other materials, like the amniotic £uid, the cord blood sample and the cerebrospinal £uid (CSF), reacted with the same spots [3,7,9,10,13,16,21,25,32,35] which were most frequently detected by all four immunoglobulin classes present in the patients sera. Only a few particularities were observed: Spot 1 gave positive responses exclusively with IgG.…”

Section: Resultsmentioning

confidence: 99%

Toxoplasma gondii infection: analysis of serological response by 2-DE immunoblotting

Geißler¹

1999

FEMS Immunology and Medical Microbiology

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Toxoplasma gondii is known to cause a variety of diseases ranging from asymptomatic infections to serious conditions in immunocompromised hosts such as AIDS-patients or transplant recipients. In addition they may cause abortion or fetal abnormalities during pregnancy. Despite the clinical importance, diagnosis, treatment and prevention still remain unsatisfactory. Analysis of the parasitic cell determinants, recognized by specific humoral and cellular immune responses, may have important implications for diagnosis, therapy and vaccination strategies. Two-dimensional electrophoresis (2-DE) was used to resolve and compare protein patterns from Toxoplasma gondii strains RH and BK (mouse virulent strains). Comparison of silver-stained gels showed that 35.2% to 60.3% of the spots had the same position. In a second series of experiments, the reactivity of the spots with human sera was tested. Proteins were transferred to PVDF membranes and were detected with sera from different patient groups. Depending upon the immunoglobulin class (IgG, IgM, IgA or IgE) different epitope patterns were observed. Some of the spots seemed to be recognized in different stages of infection. Sera of two patients with similar serology and comparable stage of infection were compared in order to demonstrate an individual immune response.

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“…This infection is generally mild or asymptomatic in healthy persons, whereas acquisition during the first pregnancy severely affects the organogenesis of the fetus resulting in congenital disease [57]. A high risk of complications from T. gondii infection has also been documented in immunocompromised patients.…”

Section: Detection Of Diagnostic Markers For Infectious Diseasesmentioning

confidence: 99%

Design of proteome-based studies in combination with serology for the identification of biomarkers and novel targets

Seliger

Kellner

2002

Proteomics

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Recently proteome analysis has rapidly developed in the post-genome era and is now widely accepted as a complementary technology to genetic profiling. The improvement in the technology of both two-dimensional electrophoresis (2-DE) analysis as well as protein identification has made proteomics a valuable and powerful tool to study human diseases. A combination of conventional proteome analysis with serology has been developed as a promising experimental approach for the discovery of serological markers in different malignancies. However, the design of proteome-based studies has to be carefully performed since there are a number of critical needs for systematic and reproducible proteome analysis. In particular, the selection of tissue and its preparation represent an important step in proteome analysis. Besides the preparation of protein samples, the 2-DE and protein identification is a further critical issue. So far proteome-based technologies have been successfully used in tumor immunnology for the identification of tumor-specific autoantigens. Similarly, this technology has been employed for the detection of virulence factors, antigens and vaccine candidates in infectious diseases, as well as for the identification of diagnostic and prognostic markers, suggesting that proteome-based analysis is a promising tool for the identification of prognostic, diagnostic markers as well as for novel therapeutic targets which could be used for treatment of diseases. The integration of proteome-based approaches with data from genomic or genetic profiling will lead to a better understanding of different diseases, which will then contribute to the direct translation of the research findings into clinical practice.

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Toxoplasma gondii

Cited by 6 publications

References 23 publications

Proteomics in human disease: Cancer, heart and infectious diseases

Proteomics in human disease: Cancer, heart and infectious diseases

Toxoplasma gondii infection: analysis of serological response by 2-DE immunoblotting

Design of proteome-based studies in combination with serology for the identification of biomarkers and novel targets

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