Toxoplasma gondii antibodies sheep in Lages, Santa Catarina, Brazil, and comparison using IFA and ELISA

Sakata, Francine Bragagnolo Liz Stefen; Bellato, Valdomiro; Sartor, Amélia Aparecida; Moura, Anderson Barbosa de; Souza, Antônio Pereira de; Farias, Juliana Antunes

doi:10.1590/s1984-29612012000300004

Cited by 16 publications

(5 citation statements)

References 20 publications

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“…These data are consistent with Mendonça et al, (2013), Pereira et al, (2012); Sakata et al (2012), who found no relationship between age and seroprevalence to increase toxoplasmosis. Sakata et al (2012) suggest changes in the incidence of toxoplasmosis depending on the breed of the animal and explain that this fact may be related to the management of the animals. However, in this work no significant difference was found for the breeds analyzed Table 8 when compared through the result of IFAT and ELISA, table 2.…”

Section: Resultssupporting

confidence: 91%

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Sheep toxoplasmosis diagnose by ELISA and IFAT

Duarte

Machado²,

Costa

et al. 2020

BJAER

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Toxoplasmosis in sheep herds is a major cause of abortion, causes great economic impacts and sheep are a potential transmitters of the disease, either by meat consumption or contaminated dairy products. The diagnosis of toxoplasmosis is done by serological and molecular techniques. The reaction of indirect immunofluorescence assay (IFA) and enzyme immunoassay (ELISA) are most commonly used diagnostic methods. The seroprevalence of toxoplasmosis in sheep herd IZ was 16.86% (ELISA) and 21.51% (IFAT), the results between the two tests showed sensitivity of 77.70%, specificity of 99.25%, positive predictive value of 96.55%, negative predictive value of 94.36% and Kappa 0.83 (CI 1.0 to 0.796). Kappa index showed almost perfect agreement, reinforcing the choice of ELISA as a screening method. There was no significant statistical difference (P>0.05) of 95% in the Fisher test between races and ages of the sheep.

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Section: Resultssupporting

confidence: 91%

“…The seroprevalence was obtained through IFAT for the IZ sheep herd of 21.51%, according to table 4. Results varied for the literature for the different forms of the Brazilian states, through IFAT, varying from 7% in Paraná (MOURA et al, 2007) and 80% in Santa Catarina (SAKATA et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Sheep toxoplasmosis diagnose by ELISA and IFAT

Duarte

Machado²,

Costa

et al. 2020

BJAER

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“…Sakata et al (2012) found a seroprevalence of 56.94% among sheep in the municipality of Lages, State of Santa Catarina. This high prevalence, in comparison to that obtained in the present study, may be explained by the high humidity of the Lajes region, with greater and more regular rainfall, which may contribute towards higher viability among T. gondii oocysts in the environment.…”

Section: Resultsmentioning

confidence: 93%

“…The number of sheep to be sampledin each flock was determined individually per flock using the formula for detecting the presence of infection (THRUSFIELD, 2007). The probability of detecting at least one seropositive animal in the flock was determined with a 95% confidence level (P = 0.95) and the number of seropositive animals per flock was calculated assuming an intra-flock prevalence of 56.94% (SAKATA et al, 2012). In total, 540 animals from 63 flocks in 14 municipalities in the Sertão mesoregion were used.…”

Section: Samplingmentioning

confidence: 99%

Prevalence and risk factors for Toxoplasma gondii in sheep in the State of Paraíba, Northeastern Brazil

Correia

Feitosa

Santos

et al. 2015

Rev. Bras. Parasitol. Vet.

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The aims of this survey were to determine the flock-level and animal-level prevalences of anti-Toxoplasma gondii antibodies in sheep in the State of Paraíba, Northeastern Brazil, and to identify risk factors. Blood samples were collected from 540 sheep in 63 flocks in 14 municipalities in the Sertão mesoregion. To serologically diagnose T. gondii infection, the indirect immunofluorescent antibody test (IFAT) was used. Flocks with at least one seropositive animal were considered positive. Among the 63 flocks, 28 (44.44%) were positive, and 60 (11.11%) of the 540 animals were seropositive. Variable flock size > 25 animals was identified as a risk factor (odds ratio = 3.2; 95% CI = 1.09 -9.34; P = 0.033). The results from this survey demonstrate that T. gondii is spread among sheep in the State of Paraíba.Keywords: Toxoplasmosis, small ruminants, serology, epidemiology, control. ResumoOs objetivos deste trabalho foram determinar a prevalência de rebanhos positivos e de animais soropositivos para Toxoplasma gondii em ovinos do Estado da Paraíba, Nordeste do Brasil, bem como identificar fatores de risco. Foram colhidas amostras de sangue de 540 ovinos de 63 rebanhos em 14 municípios da mesorregião do Sertão. Para o diagnóstico sorológico da infecção por T. gondii, foi utilizado o teste de imunofluorescência indireta (RIFI). Rebanhos com pelo menos um animal soropositivo foram considerados positivos. Dos 63 rebanhos, 28 (44,44%) foram positivos, e 60 (11,11%) dos 540 animais foram soropositivos. A variável tamanho do rebanho > 25 animais foi identificada como fator de risco (odds ratio = 3,2; IC 95% = 1,09 -9,34; P = 0,033). Os resultados deste trabalho sugerem a disseminação de T. gondii em ovinos no Estado da Paraíba.

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“…The frequency of infection is extremely high in other regions of Brazil. In Santa Catarina, Sakata et al (2012) detected an occurrence of 56.9%. Ferreira et al (2016) reported an occurrence of 41.3% in sheep from Rio Grande do Sul.…”

Section: Resultsmentioning

confidence: 94%

Soroprevalência de Toxoplasma gondii, Neospora caninum e Brucella abortus em ovinos de Toledo, Paraná, Brasil

Bertuzzi

Weschenfelder²,

Webber³

et al. 2022

Semina: Ciênc. Agrár.

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A toxoplasmose é uma doença cosmopolita, causada pelo Toxoplasma gondii, com grande importância no contexto de produção animal e saúde pública como evidenciado nos dois maiores surtos da doença que ocorreram nas cidades brasileiras de em Santa Izabel do Ivai-PR e Santa Maria-RS. Enquanto o Neospora caninum é o agente causador da neosporose e a Brucella spp. causadora da brucelose, duas das principais causas de falhas reprodutivas em ruminantes, ocasionando problemas como abortamento, e responsáveis por significativas perdas econômicas na ovinocultura brasileira. Considerando a escassez de dados disponíveis sobre o tema para a região estudada, o objetivo do presente estudo foi avaliar a soroprevalência de anticorpos contra T. gondii, N. caninum e B. abortus em ovinos do município de Toledo, Paraná, Brasil. Durante o período de agosto de 2017 a julho de 2018 foram visitadas 22 propriedades e coletadas 240 amostras de sangue de fêmeas e machos em idade reprodutiva. Para a detecção de anticorpos contra o T. gondii e N. caninum foi utilizada a técnica de Imunofluorescência Indireta empregando ponto de corte ≥64 e ≥50, respectivamente. Para a detecção de anticorpos contra B. abortus foi realizada a triagem com o Antígeno Acidificado Tamponado e confirmação das amostras reagentes com o teste de Soroaglutinação Lenta e do 2-Mercaptoetanol. Das amostras analisadas, 27,08% (65/240) apresentaram anticorpos contra o T. gondii, estes se encontravam distribuídas em 81,82% (18/22) das propriedades, para N. caninum observou-se uma prevalência de 15,41% (37/240), distribuídas em 68,18% (15/22) das propriedades. Quanto ao resultado dos testes da B. abortus todas as amostras foram consideradas negativas. O presente estudo mostrou que a grande maioria das propriedades apresentaram animais positivos para os protozoários, os quais, principalmente T. gondii, poderiam estar causando problemas de abortamentos. Estes dados epidemiológicos fornecem embasamento para programas estratégicos a fim de realizar medidas de monitoramento e controle, evitando perdas econômicas na ovinocultura.

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Toxoplasma gondii antibodies sheep in Lages, Santa Catarina, Brazil, and comparison using IFA and ELISA

Cited by 16 publications

References 20 publications

Sheep toxoplasmosis diagnose by ELISA and IFAT

Sheep toxoplasmosis diagnose by ELISA and IFAT

Prevalence and risk factors for Toxoplasma gondii in sheep in the State of Paraíba, Northeastern Brazil

Soroprevalência de Toxoplasma gondii, Neospora caninum e Brucella abortus em ovinos de Toledo, Paraná, Brasil

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