Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis

Pardini, Lais Luján; Moré, Gastón; Rudzinski, Marcelo; Gos, María Laura; Campero, Lucía María; Meyer, Alejandro; Bernstein, Mariana; Unzaga, Juan Manuel; Venturini, Marı́a Cecilia

doi:10.1016/j.exppara.2016.03.006

Cited by 14 publications

(15 citation statements)

References 19 publications

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“…Additionally, different regions showed different predominance of genotypes (BERNSTEIN et al, 2018). The samples obtained in our study correlates with other samples obtained from the same region/province (BASSO et al, 2009;BERNSTEIN et al, 2018;MORE et al, 2010;PARDINI et al, 2016).…”

Section: Discussionsupporting

confidence: 87%

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Toxoplasma gondii and Neospora caninum infections in synanthropic rodents from Argentina

Dellarupe

Fitte

Pardini

et al. 2019

Rev. Bras. Parasitol. Vet.

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Toxoplasma gondii and Neospora caninum are closely related coccidian parasites (phylum Apicomplexa). This is the first study from urban synanthropic rodent species that involved serological and molecular diagnosis of T. gondii and N. caninum infection, and genotyping of T. gondii in Argentina. A total of 127 rodent samples were trap captured: Mus musculus (n = 78), Rattus norvegicus (n = 26) and Rattus rattus (n = 23). Antibodies against T. gondii and N. caninum were detected by IFAT in 32.8% (40/122) and 0.8% (1/122) of rodent samples, respectively, demonstrating contact with these protozoans. Additionally, T. gondii DNA was detected in 3.3% (4/123) of rodent central nervous system samples and 2 samples were genotyped by multilocus nPCR-RFLP. Neospora caninum DNA was not detected by PCR. The 2 genotyped samples were type III allele for all markers except for SAG-1 (type I for Rat1Arg and type II/III for Rat2Arg) and were identified as #48 and #2 (likely) according to the allele combinations reported on Toxo DB (Toxo-DB). The results of the present study revealed a wide distribution of T. gondii and less for N. caninum, in synanthropic rats and mice in the studied area.

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Section: Discussionsupporting

confidence: 87%

“…In Argentina, several studies have been conducted to isolate and genotype T. gondii from different animal species from different regions (BASSO et al, 2009;MORE et al, 2012;PARDINI et al, 2016). As stated before, samples from Buenos Aires province showed clonal genotypes as well as non-clonal genotypes grouped with type III lineage.…”

Section: Discussionmentioning

confidence: 95%

Toxoplasma gondii and Neospora caninum infections in synanthropic rodents from Argentina

Dellarupe

Fitte

Pardini

et al. 2019

Rev. Bras. Parasitol. Vet.

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“…ND, no data; B, brain; H, heart; Li, liver; Lu, lung; Sk, skeletal muscle udy from Argentina (Pardini et al, 2016;Bernstein et al, 2018) also had success in isolating good quality DNA from the brains of two naturally infected chickens from Argentina; one sample was ToxoDB genotype #19, the other was #286 based on 10 PCR-RFLP markers (Table 4). Histopathology and immunohistochemistry: The T. gondii burden in tissues of asymptomatic chickens is low (Dubey, 2010a).…”

Section: Parasitologic Investigationmentioning

confidence: 99%

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Dubey¹,

Pena²,

Cerqueira‐Cézar³

et al. 2020

Parasitology

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Toxoplasma gondii infections are common in humans and animals worldwide. Domestic free-range chickens (Gallus domesticus) are excellent sentinels of environmental contamination with T. gondii oocysts because they feed on the ground. Chickens can be easily infected with T. gondii; however, clinical toxoplasmosis is rare in these hosts. Chickens are comparatively inexpensive and thus are good sentinel animals for T. gondii infections on the farms. Here, the authors reviewed prevalence, the persistence of infection, clinical disease, epidemiology and genetic diversity of T. gondii strains isolated from chickens worldwide for the past decade. Data on phenotypic and molecular characteristics of 794 viable T. gondii strains from chickens are discussed, including new data on T. gondii isolates from chickens in Brazil. This paper will be of interest to biologists, epidemiologists, veterinarians and parasitologists.

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“…Clonal type II genotypes predominate in Chile different to the patterns observed in South America (Rajendran et al, 2012). In Argentina, non-clonal allele combinations of type II and type III genotypes (using 9 markers) have been described in isolates obtained from zoo animals like meerkats, kangaroos, wallabies and monkeys (Basso et al, 2009;Basso et al, 2007;Moré et al, 2010;Pardini et al, 2015) and also from domestic animals such as pigs, rabbits and chickens from different regions of the country (Bacigalupe et al, 2011;Moré et al, 2012;Pardini et al, 2011;Pardini et al, 2016). In southern Brazil a high rate of human ocular toxoplasmosis (five times higher than reported in Europe) has been reported and is suggested to be associated with atypical genotypes infection (Silveira et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…In the state of Minas Gerais it was found that genotypes isolated from humans overlap with those found in domestic animals, suggesting a common source of infection between them (Silva et al, 2014). In Argentina, in the central and east region of Misiones province (near Brazil), the rate of patients with ocular toxoplasmosis is around 20% of clinic attendees (Rudzinski and Meyer, 2011) and non-clonal isolates of T. gondii were obtained from chickens belonging to the farms of the (Pardini et al, 2016). In addition, T. gondii isolates with non-clonal genotypes were obtained from congenital toxoplasmosis cases in the country (Pardini et al, in preparation).…”

Section: Introductionmentioning

confidence: 99%

Population structure of Toxoplasma gondii in Argentina

Bernstein

Pardini

Moré

et al. 2018

Infection, Genetics and Evolution

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The protozoan Toxoplasma gondii is worldwide distributed showing a particular population structure that may differ among continents and countries. The aim of this study was to analyze the T. gondii population structure in Argentina and compare it with genotyping information from other South American countries. For the analysis, 39 samples from Argentina (isolates from the provinces of Buenos Aires, Misiones, Entre Ríos and San Luis) were genotyped using 10 multilocus PCR-RFLP markers including SAG1, SAG2 (5'-3'SAG2, alt. SAG2), SAG3, BTUB, GRA6, C22-8, C29-2, L358, PK1, and Apico. The T. gondii DNA samples were obtained from domestics animals (chickens n = 20; cats n = 3; pigs n = 2; goat n = 1; rabbit n = 1), humans (n = 6), zoo animals (n = 5) and a rat (n = 1). Phylogenetic relationship of these Argentinean isolates together with representative reference genotypes was determined by phylogenetic network analysis. Thirty-seven Argentinean samples belonged to 21 genotypes and two samples were genotyped at 8 of the 10 loci and considered incomplete characterized. Among these 37 typed samples, five genotypes were not previously reported. The majority of the samples grouped with the Type III (ToxoDB PCR-RFLP genotype #2) lineage. The clonal Type II (ToxoDB genotypes #1 and #3) was also identified. Our results suggest a unique population structure with combination of unique genotypes and the common Type II and Type III lineages in Argentina. Nevertheless, different regions showed distinctive pattern of genotypes, revealing a higher variability in Northern provinces.

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Toxoplasma gondii isolates from chickens in an area with human toxoplasmic retinochoroiditis

Cited by 14 publications

References 19 publications

Toxoplasma gondii and Neospora caninum infections in synanthropic rodents from Argentina

Toxoplasma gondii and Neospora caninum infections in synanthropic rodents from Argentina

Epidemiologic significance ofToxoplasma gondiiinfections in chickens (Gallus domesticus): the past decade

Population structure of Toxoplasma gondii in Argentina

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