Toxoplasma gondii recombinant antigens H4 and H11: Use in ELISAs for detection of toxoplasmosis in swine

Andrews, C.D.; Dubey, J. P.; Tenter, Astrid M.; Webert, Donald W.

doi:10.1016/s0304-4017(96)01154-5

Cited by 22 publications

(8 citation statements)

References 18 publications

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“…Several T. gondii antigens have already been used as fusion proteins to develop serological tools based only on the detection of acute and chronic IgG and IgM antibodies (1,12,14,15,19,21,28). Among them, recombinant P22 (glutathione S-transferase-P22 fusion) proved to be an acute-infection marker, detecting anti-P22 IgG in sera from acutely infected individuals more strongly than in those from chronically infected individuals (19).…”

Section: Discussionmentioning

confidence: 99%

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Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Martin

Arcavi

Santillán

et al. 1998

Clin Diagn Lab Immunol

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The Toxoplasma gondii rhoptry protein Rop2 was expressed in Escherichia coli as a fusion protein containing 44 kDa of the 55-kDa mature Rop2, supplied with six histidyl residues at the N-terminal end (Rop2196–561). Humoral response during Toxoplasma infection of humans was analyzed by immunoglobulin G (IgG), IgA, and IgM enzyme-linked immunosorbent assay with Rop2196–561 as the antigen substrate. The analyzed sera were divided according to T. gondii-specific serological tests (IgG, IgA, or IgM indirect immunofluorescence and IgA or IgM immunosorbent agglutination assay) as group A (IgG+ IgA− IgM−;n = 35), group B (IgG+ IgA+IgM+; n = 21), group C (IgG+IgA+ IgM−; n = 5), and group D (IgG+ IgA− IgM+;n = 16). Twenty-six T. gondii-seronegative sera from individuals with other infections were also included (group E). Anti-Rop2 IgG antibodies were detected in 82.8% of group A sera and in 97.6% of the sera with acute-phase marker immunoglobulins (groups B, C, and D). The percentage of IgA antibody reactivity against Rop2196–561 was 17.1% in group A, 50% in group D, and 80.8% in groups B and C. The percentage of IgM antibody reactivity was 0% in groups A and C and 62% in groups B and D. Sera from group E failed to show IgA, IgM, or IgG antibody reactivity. Since T. gondii Rop2 elicits a strong humoral response from an early stage of infection, it is suggested that recombinant Rop2196–561 would be suitable for use in diagnostic systems, in combination with other T. gondii antigens, to detect specific IgG, IgA, and IgM antibodies.

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Section: Discussionmentioning

confidence: 99%

“…However, the use of whole-tachyzoite antigens can result in false-positive reactions (13,27). Therefore, recent advances have been made in generating recombinant antigens of T. gondii which are less expensive and easier to standardize in IgG and IgM serological tests (1,8,12,14,15,19,21,28).…”

mentioning

confidence: 99%

Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Martin

Arcavi

Santillán

et al. 1998

Clin Diagn Lab Immunol

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“…This rare modification to the heteroxenous (two host) life cycle is thought to have occurred relatively recently and may be responsible for the expansion of this parasite to nearly every continent [ 1 ]. Parasite transmission via the oocyst stage has resulted in epidemics of human toxoplasmosis [ 2 - 6 ] and widespread infections of livestock that can also lead to human infections through the consumption of tissue cyst-contaminated food [ 7 , 8 ]. Together, oocyst and tissue cyst sources contribute to rates of human exposure such that the risk of infection in the U.S. is one in three by age 50 (25% for >20 yrs of age [ 9 ]; and nearly 100% by the end of childhood in other parts of the world [ 10 ]).…”

Section: Introductionmentioning

confidence: 99%

The transcriptome of Toxoplasma gondii

et al. 2005

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Background: Toxoplasma gondii gives rise to toxoplasmosis, among the most prevalent parasitic diseases of animals and man. Transformation of the tachzyoite stage into the latent bradyzoite-cyst form underlies chronic disease and leads to a lifetime risk of recrudescence in individuals whose immune system becomes compromised. Given the importance of tissue cyst formation, there has been intensive focus on the development of methods to study bradyzoite differentiation, although the molecular basis for the developmental switch is still largely unknown.

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“…A comparação entre as raças também mostrou diferença significativa na ocorrência de anticorpos, sendo 38.3% (23/ 60) (ANDREWS et al, 1997). Usually, T. gondii does not produce clinic signals, but the primary infection during pregnancy in women and a few animal species may result in abortion, fetal abnormalities or perinatal death (COOK et al, 2000).…”

Section: Toxoplasma Gondii é Um Parasito Reconhecido Mundialmente Commentioning

confidence: 99%

Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume

Macedo

Barros

et al. 2012

Cienc. Rural

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Toxoplasma gondii recombinant antigens H4 and H11: Use in ELISAs for detection of toxoplasmosis in swine

Cited by 22 publications

References 18 publications

Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

The transcriptome of Toxoplasma gondii

Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume

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