Toxoplasmosis prevalence in Egyptian diabetic patients

Hemida, Mahmoud Haddad; Shahat, Samir A; Bayoumy, Ahmed M. S.; Mohamed, Khairy A.; Hassan, Shady M

doi:10.4103/azmj.azmj_53_18

Cited by 3 publications

(3 citation statements)

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“…Nayeri et al 15 , reported salivary IgG, IgM, and IgA rates of 18.66%, 15%, and 49.18%, respectively, in Iran. In Egypt, IgG seropositivity rates were 46% and 45% among diabetic patients according to studies by Hemida et al 48 and Khattab et al 49 , respectively. Pregnant women in Nigeria exhibited low rates of IgG and IgM (26.6% and 5.7%, respectively) 50 .…”

Section: Resultsmentioning

confidence: 94%

In silico study to explore the mechanism of Toxoplasma-induced inflammation and target therapy based on sero and salivary Toxoplasma

Hassanein,

Fadel,

Shehata

et al. 2024

Sci Rep

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We aimed to assess salivary and seroprevalence of Toxoplasma immunoglobulins in risky populations and evaluate drug docking targeting TgERP. A cross-sectional study was conducted in Alexandria University hospitals’ outpatient clinics. 192 participants were enrolled from September 2022 to November 2023. Anti-Toxoplasma IgG and IgM were determined in serum and saliva by ELISA. An in-Silico study examined TgERP’s protein–protein interactions (PPIs) with pro-inflammatory cytokine receptors, anti-inflammatory cytokine, cell cycle progression regulatory proteins, a proliferation marker, and nuclear envelope integrity-related protein Lamin B1. Our findings revealed that anti-T. gondii IgG were detected in serum (66.1%) and saliva (54.7%), with 2.1% of both samples were positive for IgM. Salivary IgG had 75.59% sensitivity, 86.15% specificity, 91.40% PPV, 64.40% NPP, 79.17% accuracy and fair agreement with serum IgG. On the other hand, the sensitivity, specificity, PPV, NPV, and accuracy in detecting salivary IgM were 75.0%, 99.47%, 75.0%, 99.47%, and 98.96%. AUC 0.859 indicates good discriminatory power. Examined synthetic drugs and natural products can target specific amino acids residues of TgERP that lie at the same binding interface with LB1 and Ki67, subsequently, hindering their interaction. Hence, salivary samples can be a promising diagnostic approach. The studied drugs can counteract the pro-inflammatory action of TgERP.

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Section: Resultsmentioning

confidence: 94%

In silico study to explore the mechanism of Toxoplasma-induced inflammation and target therapy based on sero and salivary Toxoplasma

Hassanein,

Fadel,

Shehata

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Nayeri et al (2022) 11 , in Iran, reported rates of 18.66%, 15%, and 49.18%, for salivary IgG, IgM, and IgA antibodies respectively. In Egypt, the seropositivity for IgG was 46% and 45% among diabetic patients in the two studies carried by 41,42 . Low rates for IgG and IgM (26.6% and 5.7%, respectively) were also detected among pregnant women in Nigeria 43 .…”

Section: Serological Analysismentioning

confidence: 93%

“…(Table 3). On the other hand, several studies reported that there was no statistical difference in seroprevalence between rural and urban areas (p-value <0.05) 41,42,48,52 . In Brazil, Avelar and his colleagues 53 reported that the seroprevalence among pregnant women residing in rural areas was greater compared to those who reside in urban communities, they contributing these results to low socioeconomic levels, difficulties in obtaining health services, high exposure, and a lack of awareness about the mode of transmission.…”

Section: Risk Factors Associated With Toxoplasmosis Among the Samples...mentioning

confidence: 93%

Sero and Salivary Toxoplasma Immunoglobulins: In Silico Study on Anti-genicity-Based Therapies Docked TgERP and its Dysregulatory Action on Immunoglobulins Running title: Sero and salivary toxoplasmosis and drug docking

Hassanein,

Fadel,

Shehata

et al. 2024

Preprint

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We aimed to assess salivary and seroprevalence of toxoplasma immunoglobulins in risky populations and evaluate drug docking targeting TgERP. A Cross-sectional study was conducted in Alexandria University hospitals' outpatient clinics. 192 participants were enrolled from September 2022 to November 2023. Anti-Toxoplasma IgG and IgM were determined in serum and salivary by ELISA. An in-Silico study examined TgERP's protein-protein interactions with pro-inflammatory cytokine receptors, anti-inflammatory cytokine, cell cycle progression regulatory proteins, a proliferation marker, and nuclear envelope integrity-related protein Lamin B1. Our findings revealed that anti-T. gondii IgG were detected in serum (66.1%) and saliva (54.7%), with 2.1% of both samples were positive for IgM. Salivary IgG had 75.59% sensitivity, 86.15% specificity, 91.40% PPV, 64.40% NPP, 79.17% accuracy and fair agreement with serum IgG. On the other hand, the sensitivity, specificity, PPV, NPV, and accuracy in detecting salivary IgM were 75.0%, 99.47%, 75.0%, 99.47%, and 98.96%. AUC 0.859 indicates good discriminatory power. Examined synthetic drugs and natural products can target specific amino acids residues of TgERP that lie at the same binding interface with Lamin B1 and Ki67, subsequently, hindering their interaction. Hence, salivary samples can be a promising diagnostic approach. In addition, this study may help alleviate toxoplasmosis' inflammatory effects.

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Toxoplasmosis: An overlooked cause of confusion in a patient with myasthenia gravis

Lee

Sethi

Guy

2020

Clinical Infection in Practice

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Toxoplasmosis prevalence in Egyptian diabetic patients

Cited by 3 publications

References 0 publications

In silico study to explore the mechanism of Toxoplasma-induced inflammation and target therapy based on sero and salivary Toxoplasma

In silico study to explore the mechanism of Toxoplasma-induced inflammation and target therapy based on sero and salivary Toxoplasma

Sero and Salivary Toxoplasma Immunoglobulins: In Silico Study on Anti-genicity-Based Therapies Docked TgERP and its Dysregulatory Action on Immunoglobulins Running title: Sero and salivary toxoplasmosis and drug docking

Toxoplasmosis: An overlooked cause of confusion in a patient with myasthenia gravis

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