TPPP3 inhibits the proliferation, invasion and migration of endometrial carcinoma targeted with miR‐1827

Abstract

Objective and design Database screening indicated that tubulin polymerization‐promoting protein 3 (TPPP3) was involved in pathogenesis of multiple cancer types. miR‐1827 has a potential role in a variety of human cancers. However, the role of TPPP3 and its underlying molecular mechanism in endometrial cancer (EC) has not been investigated. Herein, we aimed to reveal the role of TPPP3/miR‐1827 in EC progression. Methods Tumour tissue and whole blood samples were collected for the detection of TPPP3 expression. TPPP3 shRNAs and pcDNA‐TPPP3 were applied to knockdown or upregulate the TPPP3 expression, and miR‐1827 mimic was used to upregulate miR‐1827 level. CCK‐8 and colony assays were applied to estimate the cell proliferation. Wound healing and Transwell assays were conducted to assess the cell migration and invasion abilities. The dual‐luciferase reporter assay was conducted to validate the putative binding site between TPPP3 and miR‐1827. Expression of TPPP3, miR‐1827 and related proteins in cell lines, tissue and whole blood sample were detected using western blot, RT‐qPCR and immunofluorescence. Results TPPP3 was observed markedly elevated in EC patients and cells. TPPP3 knockdown displayed evident suppression in cell proliferation, migration and invasion in vitro and in vivo. Moreover, we identified TPPP3 as a direct and functional target gene of miR‐1827 in EC cells. The miR‐1827 induced regulatory effects on EC cells were partially reversed by TPPP3. Additionally, in vivo study confirmed the findings discovered in vitro. Conclusion TPPP3 exerted oncogenic roles in EC progression by sponging miR‐1827. This finding might provide potential targets for EC therapy.

The Role of miRNAs in the Regulation of Endometrial Cancer Invasiveness and Metastasis—A Systematic Review

“…Moreover, 35 studies investigated the role of miRNAs in the regulation of tumor growth and EC metastasis in vivo in murine models [ 18 , 19 , 24 , 36 , 41 , 42 , 44 , 45 , 49 , 51 , 52 , 53 , 56 , 62 , 69 , 70 , 72 , 73 , 92 , 93 , 101 , 111 , 117 , 119 , 122 , 126 , 128 , 136 , 137 , 144 , 145 , 151 , 157 , 160 , 166 ]. Eight miRNAs were identified as oncomiRNAs in vivo (miR-106a, miR-107-5p, miR-130b, miR-183, miR-222-3p, miR-494-3p, miR-544a, and miR-652) and 27 as tumor suppressor miRNAs (miR-23a, miR-23b, miR-26a, miR-29a-5p, miR-29b, miR-34a, miR-34b, miR-99a, miR-101, miR-129, miR-142, miR-148b, miR-194, miR-199a/b-5p, miR-200c, miR-204, miR-214-3p, miR-302a-5p, miR-326, miR-361, miR-367-3p, miR-372, miR-449a, miR-490-3p, miR-499a, miR-505, and miR-1827).…”

Endometrial proteomic profile of patients with repeated implantation failure

“…Microtubule is the main component of mitotic spindle, which controls cell division and chromosome segregation (50). Studies have revealed that TPPP3 gene can inhibit cell proliferation, induce apoptosis and cell cycle arrest, and inhibit tumor growth (51)(52)(53). TPPP3 protein is also involved in the development and regeneration of musculoskeletal and nervous system (54,55).…”

Circ_0000808 promotes the development of non-small cell lung cancer by regulating glutamine metabolism via the miR-1827/SLC1A5 axis

“…In this, we confirmed that circ_0000808 could act as miR-1827 sponge in NSCLC. MiR-1827 acted as a tumor suppressor to hinder the development of many cancers, including hepatocellular carcinoma [27,28], colorectal cancer [29], and endometrial cancer [30]. In the past research, miR-1827 had been found to inhibit the lung cancer cell growth, metastasis, and angiogenesis [31][32][33].…”