TPX2 siRNA regulates growth and invasion of esophageal cancer cells

Liu, Hongchun; Zhang, Gen-Hao; Liu, Yuhan; Wang, Pan; Su, Lisha; Li, Shenglei; Zhang, Lan; Liu, Junwen

doi:10.1016/j.biopha.2014.08.008

Cited by 21 publications

(17 citation statements)

References 29 publications

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“…[ 15 ] Others have noted the knockdown of TPX2 significantly represses invasion and metastasis, while facilitating the apoptosis of EC cells, indicating it may serve as a novel molecular target for the treatment of EC. [ 28 ]…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA LINC00337 induces autophagy and chemoresistance to cisplatin in esophageal squamous cell carcinoma cells via upregulation of TPX2 by recruiting E2F4

et al. 2020

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Esophageal cancer represents the eighth most frequently occurring cancer, as well as the sixth most widespread cause of cancer-related deaths. In recent years, accumulating evidence has implicated long non-coding RNAs in the progression of esophageal squamous cell carcinoma (ESCC). The aim of the present study was to investigate the potential involvement and underlying mechanisms of LINC00337 in ESCC. Expression patterns of LINC00337 and targeting protein for Xenopus kinesin-like protein 2 (TPX2) in ESCC tissues and cells were detected using RT-qPCR and immunohistochemical staining. Next, the interactions among LINC00337, E2F4, and TPX2 were identified using chromatin immunoprecipitation, dual-luciferase reporter, and RNA-binding protein immunoprecipitation assays, suggesting that LINC00337 could recruit E2F4 to enhance the transcription of TPX2. Thereafter, the regulatory roles of LINC00337 and TPX2 in ESCC were analyzed by altering the expression of LINC00337 or TPX2 in ESCC cells following treatment with cisplatin (DDP). The levels of autophagy-related proteins Beclin1 and LC3II/LC3I, viability, autophagy, apoptosis, and chemoresistance of ESCC cells to DDP were measured following transfection treatment with different plasmids. Additionally, the role of the LINC00337/E2F4/TPX2 axis was assessed in vivo by measuring tumor formation in nude mice. The results demonstrated that LINC00337 upregulated TPX2, consequently leading to elevated levels of Beclin1 and LC3II/LC3I, promoted cell viability and autophagy, while inhibiting apoptosis and chemosensitivity to DDP in ESCC. In sum, the current study evidenced that the overexpression of LINC00337 6056 | YANG et Al.

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Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA LINC00337 induces autophagy and chemoresistance to cisplatin in esophageal squamous cell carcinoma cells via upregulation of TPX2 by recruiting E2F4

et al. 2020

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“…Liu et al. have reported that TPX2 siRNA promotes apoptosis of oesophageal cancer cells . Apoptosis is one of the mechanisms of radiosensitive cells in response to radiation therapy .…”

Section: Discussionmentioning

confidence: 99%

“…TPX2 expression has been reported to be upregulated in many malignancies, including squamous cell lung cancer, oesophageal cancer, hepatocellular carcinoma and breast cancer . Additionally, TPX2 expression is associated with tumour grade, clinical stage and metastasis . However, whether TPX2 is correlated with the radioresistance of human lung squamous carcinoma has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Targeting protein for Xenopus kinesin‐like protein 2 knockdown enhances radiation sensitivity of human lung squamous carcinoma cell

Yang

Gao

et al. 2017

Clin Exp Pharma Physio

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The targeting protein for Xenopus kinesin-like protein 2 (TPX2) has been demonstrated to be associated with the tumourigenesis of many cancers. In the present study, we investigated the role and preliminary mechanism of TPX2 in the resistance of lung squamous carcinoma to radiation therapy. The results showed that SK-MES-1R and NCI-H226R cells were more resistant to X-ray irradiation than the parental cells (SK-MES-1 and NCI-H226). Moreover, TPX2 was upregulated in the radioresistant cells compared with the parental cells. TPX2 knockdown significantly decreased TPX2 expression in SK-MES-1 cells, while TPX2 overexpression increased TPX2 expression in NCI-H226 cells compared with the corresponding control cells. TPX2 knockdown enhanced the radiosensitivity of SK-MES-1 and promoted cell apoptosis following exposure to irradiation, whereas TPX2 overexpression decreased the radiosensitivity of NCI-H226 and inhibited cell apoptosis. In in vivo studies, the combination of TPX2 knockdown and irradiation significantly inhibited tumour growth, decreased tumour weight, downregulated TPX2 expression in tumour tissue and induced cell apoptosis in nude mice, while TPX2 overexpression exerted an opposite effect. Our results indicated that TPX2 was correlated with cell radioresistance and it might be served as a therapeutic target to enhance cell radiosensitivity in the radiation therapy of lung squamous carcinoma.

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“…ece.ntua.gr/micro-CDS/), we focused on TPX2. Three previous studies showed that TPX2 is an oncogene and plays an important role in ESCC progression [17][18][19][20].…”

Section: Tpx2 Is a Potential Target Gene Of Mir-491mentioning

confidence: 99%

“…TPX2 regulates tumor growth in human cervical carcinoma cells [16]. siRNA/TPX2 could inhibit the invasion and metastasis of EC cells, and may become a new approach for treatment of esophageal carcinoma [17].…”

Section: Introductionmentioning

confidence: 99%

Low Expression of miR-491 Promotes Esophageal Cancer Cell Invasion by Targeting TPX2

Niu

Gong

Tian

et al. 2015

Cell Physiol Biochem

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Background/Aims: MicroRNAs (miRNAs) have a key role in carcinogenesis and cancer development, but the role of miRNAs in the progression of esophageal cancer (EC) remains unclear. Methods: The TE-1 and Eca-109 EC cell lines were used. The expression of miR-491 and candidate gene TPX2 in EC samples (n=99) were detected by RT-PCR. The cells invasive ability was determined by transwell assay. The luciferase reporter assay was used to confirm the regulation mechanism. Results: A decreased expression of miR-491 was detected in the EC clinical samples compared with the corresponding adjacent tumor tissues. Aberrant expression of miR-491 regulated cells invasion and EMT markers. Furthermore, we verified that TPX2 was a target gene of miR-491. Conclusions: miR-491 may play a critical role in EC.

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TPX2 siRNA regulates growth and invasion of esophageal cancer cells

Cited by 21 publications

References 29 publications

Long non‐coding RNA LINC00337 induces autophagy and chemoresistance to cisplatin in esophageal squamous cell carcinoma cells via upregulation of TPX2 by recruiting E2F4

Long non‐coding RNA LINC00337 induces autophagy and chemoresistance to cisplatin in esophageal squamous cell carcinoma cells via upregulation of TPX2 by recruiting E2F4

Targeting protein for Xenopus kinesin‐like protein 2 knockdown enhances radiation sensitivity of human lung squamous carcinoma cell

Low Expression of miR-491 Promotes Esophageal Cancer Cell Invasion by Targeting TPX2

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