Trabecular bone deterioration at the greater trochanter of mice with unilateral obstructive nephropathy

Abstract

Our previous study showed the early molecular responses of bone in response to obstructive nephropathy in a unilateral ureteral obstruction (UUO) mouse model. Here, we addressed the changes in trabecular bone properties at greater trochanter, the proximal and the distal metaphysis of femur in UUO mice. The male mice were subjected to UUO (n510) or sham operation (n510). All mice were killed on day 7 after the surgical operation. The micro-computed tomography (micro-CT) analysis for different femoral trabecular bone sites demonstrated pathological alterations of trabecular bone mass and micro-networks at greater trochanter as shown by decreases in bone mineral density/bone volume (P,0.05) and trabecular number (P,0.05) and increases in trabecular separation (P,0.01) and bone surface/bone volume (P,0.05) in UUO mice. The present study demonstrates that UUO-induced unilateral obstructive nephropathy has markedly detrimental effects on the trabecular trochanter of the femur. 1 The TMV system provides relevant information and is widely used to evaluate CKD patient bone health; 2 however, this information should be expanded to include cancellous bone architecture.Unilateral ureteral obstruction (UUO) induces damage in the unilateral obstructed kidney and is a well-established model of tubulointerstitial kidney fibrosis. 3 We previously reported changes in the renal expression of vitamin D metabolic enzymes and changes in calcium transporter abundance following obstructive nephropathy in mice subjected to UUO 4 and demonstrated early molecular responses of bone to unilateral obstructive nephropathy in the UUO model. 5 However, associated with the pathological alterations of vitamin D metabolism and the expression of genes involved in bone metabolism, whether trabecular bone microstructure would deteriorate in UUO mice remained to be clarified.The availability of three-dimensional (3D) measuring techniques opens new avenues to analyse the biological properties of bone. In this study, femoral trabecular bone parameters were determined by microcomputed tomography (micro-CT) to qualitatively illustrate the alterations of bone properties in mice that were subjected to unilateral obstructive nephropathy.

Macrophages Promote Osteoblastic Differentiation In Vivo: Implications in Fracture Repair and Bone Homeostasis

“…Hand‐drawn contours were used to isolate the regions of interest for both cortical and trabecular compartments. Two volumes of interest (VOI) were established: a 1 mm VOI at the mid‐diaphysis of the femur for cortical bone, and a 50‐section VOI in the proximal metaphysis region for trabecular bone, as described elsewhere . Reconstructed 3D images were generated using CTvol (Skyscan Bruker MicroCT).…”

Cardiac transcriptomic changes induced by early CKD in mice reveal novel pathways involved in the pathogenesis of Cardiorenal syndrome type 4

“…In this study, we only evaluated heart tissue, but transcriptomic changes induced by UUO probably occur in other organs. Though UUO is a widely used model of tubulointerstitial kidney fibrosis, multiple studies have reported changes in lung function, bone metabolism and structure, and cognitive and neuropathological damage following obstructive nephropathy [ [57] , [58] , [59] ]. Hence, the global changes occurring after UUO cannot be underestimated but instead studied further to understand and treat better the systemic complications of renal diseases.…”

Chronic kidney disease to osteoporosis: histopathological analysis on animal model with unilateral ureteral obstruction method

“…A previous study analyzing bone histopathology in a Sprague Dawley rat model that was given adenine for 4 weeks to induce CKD and a high-phosphorus diet for 38 weeks showed changes in trabeculation of loose and thin-walled bones, which characterizes osteoporosis (Ni et al, 2018). Another study using the ICR model of CKD mice induced by the UUO method for 7 days showed a decrease in trabecular bone mass in the trochanteric part of the mouse femur (Gu et al, 2013). The current study using male white rats of the Wistar strain (Rattus norvegicus) induced by the UUO method to produce a CKD model showed that the most distant gap between trabecular bone tissue, demonstrating the osteoporosis process, was on the 28th day after CKD modeling.…”

“…A previous study analyzing bone histopathology in a Sprague Dawley rat model that was given adenine for 4 weeks to induce CKD and a high-phosphorus diet for 38 weeks showed changes in trabeculation by loose and thin-walled bones, which characterizes osteoporosis (Ni et al, 2018). Another study using the Institute of Cancer Research (ICR) model of CKD mice induced by the UUO method for 7 days showed a decrease in trabecular bone mass in the trochanteric part of the mice femur, indicating sensitivity to intervention using the UUO method (Gu et al, 2013). Data regarding the onset progression of bone pathological conditions in experimental animal models with CKD, particularly in Wistar rats induced by the UUO method, are still limited.…”