Trabecular Titanium Induces Osteoblastic Bone Marrow Stem Cells Differentiation

Abstract

Pure titanium and titanium alloys are materials widely used in orthopedics and dental surgery because of their mechanical properties, chemical stability and biocompatibility. Although excellent clinical results have been shown, traditional porous metals have several inherent limitations (low volumetric porosity, relatively high modulus of elasticity and availability as a coating only). With the aim of going over these limits, improving the potentiality of osteointegration, a new highly porous titanium biomaterial (Trabecular Titanium™, TT) has been developed. Because the molecular events due to TT and able to alter osteoblast activity to promote bone formation are poorly understood, expression of osteoblastic related genes in mesenchymal stem cells exposed to TT was investigated.The expression levels of bone related genes like RUNX2, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1) and mesenchymal stem cells marker (CD105) were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction.TT causes induction of bone related genes osteopontin (SPP1), osteocalcin (BGLAP) alkaline phosphatase (ALPL) and indicating the differentiation effect of this biomaterial on mesenchymal stem cells.The obtained results can be relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Potency of Various Types of Stem Cells and their Transplantation

“…The use of adult stem cells isolated from patients could solve immunological problems associated to cell transplant [50]. Adult bone marrow [51] contains at least two types of stem cells: hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) [52].…”

“…Mesenchymal Stem Cells are used for cell therapy for the treatment of various neurological disorders [65]. Mesenchymal stem cells are defined as selfrenewable, multipotent progenitor cells with the ability to differentiate, under adequate stimuli, in several mesenchymal lineages [66]. MSCs are multipotent stem cells that can differentiate into a variety of cell types, including osteoblasts (bone cells), chondrocytes (cartilage cells) and adipocytes (fat cells) [67].…”

Statistics and Bioinformatics Ethnicity

“…Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. The expression levels of bone related genes like RUNX2, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1) and mesenchymal stem cells marker (CD105) were analyzed, using real time Reverse Transcription-Polymerase Chain Reaction [14].…”

Exploring the Recent Advances in Stem Cell Research

“…MSCs found to have anti-inflammatory effects and treat various painful states such as degenerative disc disease, osteoarthritis, cartilage regeneration, multiple sclerosis [13][14][15][16]. Stem cells are unique from other cell types by two important characteristics.…”