Trace amounts of antibiotic altered metabolomic and microbial profiles of weaned pigs infected with a pathogenic E. coli

Abstract

Background Our previous study has shown that supplementation of trace amounts of antibiotic exacerbated the detrimental effects of enterotoxigenic E. coli (ETEC) infection and delayed the recovery of pigs that may be associated with modified metabolites and metabolic pathways. Therefore, the objective of this study was to explore the impacts of trace levels of antibiotic (carbadox) on host metabolic profiles and colon microbiota of weaned pigs experimentally infected with ETEC F18. Results The multivariate analysis highlighted a distinct metabolomic profile of serum and colon digesta between trace amounts of antibiotic (TRA; 0.5 mg/kg carbadox) and label-recommended dose antibiotic (REC; 50 mg/kg carbadox) on d 5 post-inoculation (PI). The relative abundance of metabolomic markers of amino acids, carbohydrates, and purine metabolism were significantly differentiated between the TRA and REC groups (q < 0.2). In addition, pigs in REC group had the highest (P < 0.05) relative abundance of Lactobacillaceae and tended to have increased (P < 0.10) relative abundance of Lachnospiraceae in the colon digesta on d 5 PI. On d 11 PI, pigs in REC had greater (P < 0.05) relative abundance of Clostridiaceae compared with other groups, whereas had reduced (P < 0.05) relative abundance of Prevotellaceae than pigs in control group. Conclusions Trace amounts of antibiotic resulted in differential metabolites and metabolic pathways that may be associated with its slow responses against ETEC F18 infection. The altered gut microbiota profiles by label-recommended dose antibiotic may contribute to the promotion of disease resistance in weaned pigs.

Impact of an oligosaccharide-based polymer on the metabolic profiles and microbial ecology of weanling pigs experimentally infected with a pathogenic E. coli

“…S 2 ). This observation is consistent with our previous research [ 35 ] and research published by other labs [ 36 , 37 ]. These alterations suggest that antibiotic treatment may increase amino acid availability for the host.…”

“…Additionally, S. suis is capable of invading multiple organs to cause exaggerated inflammation, which has to be treated with antibiotics [ 71 ]. The reduced abundance of Streptococcaceae in colon digesta of pigs was also observed in our previous experiment when carbadox was administered [ 35 ].…”

Integration of the Microbiome, Metabolome and Transcriptome Reveals Escherichia coli F17 Susceptibility of Sheep

“…It is worth noting that only 11 metabolites were identified in the present study. Similar results were also obtained by He et al [ 8 ] and Kim et al [ 35 ], who found that only small subsets of metabolites were found to be differentially expressed between E. coli F18 challenged pigs and non-challenged pigs (serum, ileal mucosa and colon digest). These findings indicated that the relatively stable metabolic profile may be a global characteristic for ETEC infection, which presents the demand for a more efficient approach to discover metabolic biomarkers rather than classic statistical methods, such as the machine-learning approach.…”

A novel portable Raman scattering platform for antibiotic screening in pig urine

“…Kim et al . [ 49 ] found that cabadox concentration was the highest on day 5 in the serum and colon microbiota. Another experiment conducted with manure samples confirmed that the maximum excretion of difloxacin and sarafloxacin occurred on days 4−6 after oral administration [ 50 ].…”

“…Sequentially injected enrofloxacin on days 2−3 causes high concentrations of antibiotics in bloodstream as well as in the excretory system. Kim et al [49] found that cabadox concentration was the highest on day 5 in the serum and colon microbiota. Another experiment conducted Available at www.veterinaryworld.org/Vol.16/January-2023/25.pdf with manure samples confirmed that the maximum excretion of difloxacin and sarafloxacin occurred on days 4−6 after oral administration [50].…”